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An Expressed Sequence Tag Analysis of the Intertidal Brown Seaweeds Fucus serratus (L.) and F. vesiculosus (L.) (Heterokontophyta, Phaeophyceae) in Response to Abiotic Stressors

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Abstract

In order to aid gene discovery and uncover genes responding to abiotic stressors in stress-tolerant brown algae of the genus Fucus, expressed sequence tags (ESTs) were studied in two species, Fucus serratus and Fucus vesiculosus. Clustering of over 12,000 ESTs from three libraries for heat shock/recovery and desiccation/rehydration resulted in identification of 2,503, 1,290, and 2,409 unigenes from heat-shocked F. serratus, desiccated F. serratus, and desiccated F. vesiculosus, respectively. Low overall annotation rates (18–31%) were strongly associated with the presence of long 3′ untranslated regions in Fucus transcripts, as shown by analyses of predicted protein-coding sequence in annotated and nonannotated tentative consensus sequences. Posttranslational modification genes were overrepresented in the heat shock/recovery library, including many chaperones, the most abundant of which were a family of small heat shock protein transcripts, Hsp90 and Hsp70 members. Transcripts of LI818-like light-harvesting genes implicated in photoprotection were also expressed during heat shock in high light. The expression of several heat-shock-responsive genes was confirmed by quantitative reverse transcription polymerase chain reaction. However, candidate genes were notably absent from both desiccation/rehydration libraries, while the responses of the two species to desiccation were divergent, perhaps reflecting the species-specific physiological differences in stress tolerance previously established. Desiccation-tolerant F. vesiculosus overexpressed at least 17 ribosomal protein genes and two ubiquitin-ribosomal protein fusion genes, suggesting that ribosome function and/or biogenesis are important during cycles of rapid desiccation and rehydration in the intertidal zone and possibly indicate parallels with other poikilohydric organisms such as desiccation-tolerant bryophytes.

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Acknowledgements

This work was supported by research and technical platform access grants from the Marine-Genomics-Europe Network of Excellence (European Commission Contract No. GOCE-CT-2004-505403; JLO, GAP), as well as support from FCT-FEDER Portugal (GAP).

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Authors and Affiliations

  1. CCMAR, CIMAR-Laboratório Associado, FCMA, Universidade do Algarve, Gambelas, Faro, 8005-139, Portugal

    Gareth A. Pearson, Asuncion Lago-Leston & Ester T. A. Serrão

  2. Department of Marine Benthic Ecology and Evolution, Center for Ecological and Evolutionary Studies, University of Groningen, 9750 AA, Haren, The Netherlands

    Galice Hoarau, James A. Coyer & Jeanine L. Olsen

  3. Max Planck Institute for Molecular Genetics, Ihnestrasse 73, 14195, Berlin, Germany

    Michael Kube & Richard Reinhardt

  4. CeBiTec—Center of Biotechnology, Bielefeld University, 33594, Bielefeld, Germany

    Kolja Henckel

  5. SIG-FR2424, Centre National de la Recherche Scientifique and Université Pierre et Marie Curie, Station Biologique, BP 74, 29682, Roscoff, France

    Erwan Corre

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  1. Gareth A. Pearson
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Table S1

ESTs (TCs and singletons) from Fucus EST libraries representing genes putatively involved in cellular stress responses, based on homology with genes in the UniProt database (see text for details). The identity of the contig or EST read, number of sequence reads comprising the contig, and its total length in base pairs are shown. The identity of the gene product, an indication of its function, as well as the E-value and UniProt accession of the best hit (Blastx) are also indicated. (DOC 307 kb)

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Pearson, G.A., Hoarau, G., Lago-Leston, A. et al. An Expressed Sequence Tag Analysis of the Intertidal Brown Seaweeds Fucus serratus (L.) and F. vesiculosus (L.) (Heterokontophyta, Phaeophyceae) in Response to Abiotic Stressors. Mar Biotechnol 12, 195–213 (2010). https://doi.org/10.1007/s10126-009-9208-z

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