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Evaluation of 660 nm LED light irradiation on the strategies for treating experimental periodontal intrabony defects

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Abstract

This study aims to investigate the therapeutic value of 660 nm light-emitting diode (LED) light irradiation on the strategies for treating experimental periodontal intrabony defects in vivo. Large-sized periodontal intrabony defects were created bilaterally on the mesial aspect of the maxillary second molars of 48 Sprague–Dawley rats, and the rats were equally divided into four treatment groups with primary wound intention (n = 6/treatment/time point), including open flap debridement alone (OD), barrier membrane alone (MB), xenograft alone (BG), and xenograft plus barrier membrane (MG). Each group received daily 0 or 10 J/cm2 LED light irradiation. The animals were sacrificed after 1 or 4 weeks. The treatment outcome was evaluated by gross observation of wound dehiscence and healing, micro-CT imaging for osteogenesis, and histological assessments for inflammatory cell infiltration and periodontal reattachment. With LED light irradiation, the extent of wound dehiscence was reduced, wound closure was accelerated, epithelial downgrowth was prevented, inflammation was reduced, and periodontal reattachment was promoted in all treatment strategies. Significant reduction of inflammation with LED light irradiation was noted at 1 week in the groups BG and MG (p < 0.05). Osteogenesis was significantly promoted only in the group OD at both time points (p < 0.05). Our study showed that 660 nm LED light accelerates mucoperiosteal flap healing and periodontal reattachment. However, the enhancement of osteogenesis appeared to be limited while simultaneously treating with a barrier membrane or xenograft.

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Acknowledgments

The authors acknowledge the technical support from the Taiwan Mouse Clinics-National Phenotyping and Drug Testing Center, Dr. Min-Chun Yu, and Dr. Shan-Huey Yu for assisting in the data analysis and JETTS Technology Co. Ltd. for designing the LED device.

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Authors and Affiliations

  1. Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan

    Chih-Yun Tao, Hao-Chieh Chang & Po-Chun Chang

  2. Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, 1 Chang-Te St, Taipei, 100, Taiwan

    Ning Lee, Hao-Chieh Chang, Connie Yang, Xin-Hong Yu & Po-Chun Chang

Authors
  1. Chih-Yun Tao
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  2. Ning Lee
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  3. Hao-Chieh Chang
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  4. Connie Yang
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  5. Xin-Hong Yu
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  6. Po-Chun Chang
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Corresponding author

Correspondence to Po-Chun Chang.

Ethics declarations

All authors declare no conflict of interest relative to the study. All procedures on the animals were performed under the approved protocol no. 20130054 from the Institutional Animal Care and Use Committee of the National Taiwan University.

Source of funding

The study was supported by the research grant MOST-103-2314-B-002-095-MY2 from the Ministry of Science and Technology (Taiwan).

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The authors declare that they have no conflict of interest.

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Fig. S1

The thickness of gingiva of the edentulous ridge is generally less than 1.0 mm. Scale bar: 200 μm. Magnification: 100× (JPG 1615 kb)

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Tao, CY., Lee, N., Chang, HC. et al. Evaluation of 660 nm LED light irradiation on the strategies for treating experimental periodontal intrabony defects. Lasers Med Sci 31, 1113–1121 (2016). https://doi.org/10.1007/s10103-016-1958-z

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  • DOI: https://doi.org/10.1007/s10103-016-1958-z

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