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Effect of Er,Cr:YSGG laser application in the treatment of experimental periodontitis

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Abstract

The purpose of this study was to evaluate the influence of an erbium, chromium:yttrium–scandium–gallium–garnet (Er,Cr:YSGG) laser in the absence or presence of manual scaling and root planning (SRP) for the treatment of induced periodontitis in rats. Ligatures were placed in the subgingival region of the maxillary first molar. After a 7-day period, the ligatures were removed, and 40 rats were randomly divided into four groups (G), as follows: (GI) no treatment, (GII) scaling and root planning (SRP) with curettes, (GIII) Er,Cr:YSGG laser irradiation and (GIV) SRP with curettes followed by Er,Cr:YSGG laser irradiation. Seven and 30 days after the treatment, the animals were sacrificed and histologic, histometric and immunohistochemistry analyses were performed. All groups showed similar histopathological characteristics during the evaluation period. The histometric analysis was confirmed using Bonferroni and paired t tests. At 7 and 30 days, groups II, III and IV exhibited greater bone formation in the furcation area when compared to group I (p < 0.0001; p < 0.05). During the 7-day period, the groups irradiated with the laser (III and IV) showed a statistically larger new bone area than the group treated with SRP (II) (p < 0.01). Immunohistochemistry analysis revealed that the control group exhibited a higher expression of tartrate-resistant acid phosphatase (TRAP) and the receptor activator of nuclear factor κΒ ligand (RANKL) when compared to groups II, III and IV (p < 0.05). All treatments were able to reduce the inflammatory processes, consequently enabling the repair of periodontal tissues. The results achieved with the application of the Er,Cr:YSGG laser suggest that this laser can stimulate greater bone formation, especially over a shorter period of time.

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Acknowledgement

This study was financially supported by the São Paulo Research Foundation (FAPESP—process number 2009/00479-9, São Paulo, Brazil), the Coordination for the Improvement of Higher Educated Personnel (CAPES, Brasilia, DF, Brazil) and the National Council for Scientific and Technological Development (CNPq, Brasilia, DF, Brazil).

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Authors and Affiliations

  1. Araraquara Dental School—São Paulo State University “Júlio de Mesquita Filho”, Araraquara, São Paulo, Brazil

    Chaine Pavone, Livia Rodrigues Perussi, Guilherme José Pimentel Lopes de Oliveira, Cássio Rocha Scardueli, Joni Augusto Cirelli, Paulo Sérgio Cerri, Elcio Marcantonio Junior, Luis Carlos Spolidorio & Rosemary Adriana Chiérici Marcantonio

  2. Diagnostic and Surgery Department—Periodontology, São Paulo State University “Júlio de Mesquita Filho”—Araraquara Dental School, Humaitá Street, 1680, 14801-903, Araraquara, São Paulo, Brazil

    Rosemary Adriana Chiérici Marcantonio

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  1. Chaine Pavone
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  2. Livia Rodrigues Perussi
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  3. Guilherme José Pimentel Lopes de Oliveira
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  9. Rosemary Adriana Chiérici Marcantonio
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Correspondence to Rosemary Adriana Chiérici Marcantonio.

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Pavone, C., Perussi, L.R., de Oliveira, G.J.P.L. et al. Effect of Er,Cr:YSGG laser application in the treatment of experimental periodontitis. Lasers Med Sci 30, 993–999 (2015). https://doi.org/10.1007/s10103-014-1526-3

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  • DOI: https://doi.org/10.1007/s10103-014-1526-3

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