Skip to main content

Advertisement

SpringerLink
Log in

Treatment of periodontal disease with an Er,Cr:YSGG laser in rats exposed to cigarette smoke inhalation

  • Original Article
  • Published:
Lasers in Medical Science Aims and scope Submit manuscript

Abstract

The purpose of this study was to evaluate the erbium, chromium:yttrium-scandiumgallium-garnet (Er,Cr:YSGG) laser irradiation in the treatment of periodontitis in rats exposed to cigarette smoke inhalation (CSI). Ligatures were placed in the maxillary second molars. After a 15-day period, the ligatures were removed and 180 animals were randomly divided into six groups: (1) CSRP group—CSI and manual scaling and root planing (SRP) treatment; (2) CL group—CSI and Er,Cr:YSGG laser irradiation; (3) CSRP + L group—CSI, SRP, and Er,Cr:YSGG irradiation; (4) SRP group—manual SRP; (5) L group—Er,Cr:YSGG irradiation; (6) SRP + L group—SRP and Er,Cr:YSGG irradiation. At 7, 15, and 30 days after treatments, animals were euthanized and histologic, histometric, immunohistochemistry, and real-time PCR analyses were performed. Histometrically, no differences were observed in the SRP, L, and SRP + L groups exposed to CSI. The CSRP group showed more bone formation at 30 days than at 15 days (p < 0.01) but less bone at 30 days than the CL group at 30 days (p < 0.05). Immunohistochemical staining was positive for osteoblasts, fibroblasts, and osteoclasts. Real-time PCR showed more (vascular endothelial growth factor) VEGF expression in the L (p < 0.05) and SRP + L (p < 0.01) groups at 30 days than at 15 days and less VEGF expression in the CSRP group at 30 days than at 15 days (p < 0.05). There was no difference in fibroblast growth factor (FGF) expression. The Er,Cr:YSGG laser irradiation promotes favorable conditions for tissue repair even in animals exposed to CSI, with similar results as those achieved from manual scaling and root planing.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Williams RC (1990) Periodontal disease. N Engl J Med 322:373–382

    Article  CAS  PubMed  Google Scholar 

  2. van Winkelhoff AJ, Loos BG, van der Reijden WA, van der Velden U (2002) Porphyromonas gingivalis, Bacteroides forsythus and other putative periodontal pathogens in subjects with and without periodontal destruction. J Clin Periodontol 29:1023–1028

    Article  PubMed  Google Scholar 

  3. Socransky SS, Haffajee AD (1992) The bacterial etiology of destructive periodontal disease: current concepts. J Periodontol 63:322–331

    Article  CAS  PubMed  Google Scholar 

  4. Bergstrom J (1989) Cigarette smoking as risk factor in chronic periodontal disease. Community Dent Oral Epidemiol 17:245–247

    Article  CAS  PubMed  Google Scholar 

  5. Solomon HA, Priore RL, Bross ID (1968) Cigarette smoking and periodontal disease. J Am Dent Assoc 77:1081–1084

    Article  CAS  PubMed  Google Scholar 

  6. Baljoon M, Natto S, Bergstrom J (2005) Long-term effect of smoking on vertical periodontal bone loss. J Clin Periodontol 32:789–797

    Article  PubMed  Google Scholar 

  7. Haffajee AD, Socransky SS (2001) Relationship of cigarette smoking to attachment level profiles. J Clin Periodontol 28:283–295

    Article  CAS  PubMed  Google Scholar 

  8. Haffajee AD, Socransky SS (2001) Relationship of cigarette smoking to the subgingival microbiota. J Clin Periodontol 28:377–388

    Article  CAS  PubMed  Google Scholar 

  9. Torrungruang K, Nisapakultorn K, Sutdhibhisal S, Tamsailom S, Rojanasomsith K, Vanichjakvong O, Prapakamol S, Premsirinirund T, Pusiri T, Jaratkulangkoon O, Kusump S, Rajatanavin R (2005) The effect of cigarette smoking on the severity of periodontal disease among older Thai adults. J Periodontol 76:566–572

    Article  PubMed  Google Scholar 

  10. Gomes SC, Piccinin FB, Oppermann RV, Susin C, Nonnenmacher CI, Mutters R, Marcantonio RA (2006) Periodontal status in smokers and never-smokers: clinical findings and real-time polymerase chain reaction quantification of putative periodontal pathogens. J Periodontol 77:1483–1490

    Article  CAS  PubMed  Google Scholar 

  11. Wan CP, Leung WK, Wong MC, Wong RM, Wan P, Lo EC, Corbet EF (2009) Effects of smoking on healing response to non-surgical periodontal therapy: a multilevel modelling analysis. J Clin Periodontol 36:229–239

    Article  PubMed  Google Scholar 

  12. Kaldahl WB, Johnson GK, Patil KD, Kalkwarf KL (1996) Levels of cigarette consumption and response to periodontal therapy. J Periodontol 67:675–681

    Article  CAS  PubMed  Google Scholar 

  13. Kaldahl WB, Kalkwarf KL, Patil KD, Molvar MP, Dyer JK (1996) Long-term evaluation of periodontal therapy: I. Response to 4 therapeutic modalities. J Periodontol 67:93–102

    Article  CAS  PubMed  Google Scholar 

  14. Schwarz F, Aoki A, Becker J, Sculean A (2008) Laser application in non-surgical periodontal therapy: a systematic review. J Clin Periodontol 35:29–44

    Article  PubMed  Google Scholar 

  15. Ting CC, Fukuda M, Watanabe T, Aoki T, Sanaoka A, Noguchi T (2007) Effects of Er, Cr:YSGG laser irradiation on the root surface: morphologic analysis and efficiency of calculus removal. J Periodontol 78:2156–2164

    Article  PubMed  Google Scholar 

  16. Perussi LR, Pavone C, de Oliveira GJ, Cerri PS, Marcantonio RA (2012) Effects of the Er, Cr:YSGG laser on bone and soft tissue in a rat model. Lasers Med Sci 27:95–102

    Article  PubMed  Google Scholar 

  17. Sohn DS, Lee JS, An KM, Romanos GE (2009) Erbium, chromium:yttrium-scandium-gallium-garnet laser-assisted sinus graft procedure. Lasers Med Sci 24:673–677

    Article  PubMed  Google Scholar 

  18. Amirov MV, Grinin VM (2012) Impact of Er, Cr:YSGG-laser on the tooth root cement in the treatment of chronic periodontal disease (the results of electron microscopic studies). Stomatologiia (Mosk) 91:20–22

    CAS  Google Scholar 

  19. Kelbauskiene S, Baseviciene N, Goharkhay K, Moritz A, Machiulskiene V (2011) One-year clinical results of Er, Cr:YSGG laser application in addition to scaling and root planing in patients with early to moderate periodontitis. Lasers Med Sci 26:445–452

    Article  PubMed  Google Scholar 

  20. de Oliveira GJ, Pavone C, Sampaio JE, Marcantonio RA (2010) Influence of the angle of irradiation of the Er, Cr:YSGG laser on the morphology, attachment of blood components, roughness, and root wear: in vitro study. Lasers Surg Med 42:683–691

    PubMed  Google Scholar 

  21. de Oliveira GJ, Sampaio JE, Marcantonio RA (2010) Effects of Er, Cr:YSGG laser irradiation on root surfaces for adhesion of blood components and morphology. Photomed Laser Surg 28:751–756

    Article  PubMed  Google Scholar 

  22. Rovin S, Costich ER, Gordon HA (1966) The influence of bacteria and irritation in the initiation of periodontal disease in germfree and conventional rats. J Periodontal Res 1:193–204

    Article  CAS  PubMed  Google Scholar 

  23. de Almeida JM, Theodoro LH, Bosco AF, Nagata MJ, Oshiiwa M, Garcia VG (2008) In vivo effect of photodynamic therapy on periodontal bone loss in dental furcations. J Periodontol 79:1081–1088

    Article  PubMed  Google Scholar 

  24. Pavone C, Perussi LR, de Oliveira GJ, Scardueli CR, Cirelli JA, Cerri PS, Junior EM, Spolidorio LC, Marcantonio RA (2014) Effect of Er,Cr:YSGG laser application in the treatment of experimental periodontitis. Lasers Med Sci

  25. Cesar-Neto JB, Duarte PM, Sallum EA, Barbieri D, Moreno H Jr, Nociti FH Jr (2003) A comparative study on the effect of nicotine administration and cigarette smoke inhalation on bone healing around titanium implants. J Periodontol 74:1454–1459

    Article  CAS  PubMed  Google Scholar 

  26. Nociti Junior FH, Cesar Neto JB, Carvalho MD, Sallum EA, Sallum AW (2002) Intermittent cigarette smoke inhalation may affect bone volume around titanium implants in rats. J Periodontol 73:982–987

    Article  PubMed  Google Scholar 

  27. de Souza JA, Nogueira AV, de Souza PP, Cirelli JA, Garlet GP, Rossa C Jr (2011) Expression of suppressor of cytokine signaling 1 and 3 in ligature-induced periodontitis in rats. Arch Oral Biol 56:1120–1128

    Article  PubMed  Google Scholar 

  28. Derdilopoulou FV, Nonhoff J, Neumann K, Kielbassa AM (2007) Microbiological findings after periodontal therapy using curettes, Er:YAG laser, sonic, and ultrasonic scalers. J Clin Periodontol 34:588–598

    Article  PubMed  Google Scholar 

  29. Eberhard J, Ehlers H, Falk W, Açil Y, Albers HK, Jepsen S (2003) Efficacy of subgingival calculus removal with Er:YAG laser compared to mechanical debridement: an in situ study. J Clin Periodontol 30:511–518

    Article  PubMed  Google Scholar 

  30. Johnson GK, Guthmiller JM (2007) The impact of cigarette smoking on periodontal disease and treatment. Periodontol 2000 44:178–194

    Article  PubMed  Google Scholar 

  31. Hakki SS, Berk G, Dundar N, Saglam M, Berk N (2010) Effects of root planing procedures with hand instrument or erbium, chromium:yttrium-scandium-gallium-garnet laser irradiation on the root surfaces: a comparative scanning electron microscopy study. Lasers Med Sci 25:345–353

    Article  PubMed  Google Scholar 

  32. Tsurumaki Jdo N, Souto BH, Oliveira GJ, Sampaio JE, Marcantonio Junior E, Marcantonio RA (2011) Effect of instrumentation using curettes, piezoelectric ultrasonic scaler and Er, Cr:YSGG laser on the morphology and adhesion of blood components on root surfaces: a SEM study. Braz Dent J 22:185–192

    PubMed  Google Scholar 

  33. Akiyama F, Aoki A, Miura-Uchiyama M, Sasaki KM, Ichinose S, Umeda M, Ishikawa I, Izumi Y (2011) In vitro studies of the ablation mechanism of periodontopathic bacteria and decontamination effect on periodontally diseased root surfaces by erbium:yttrium-aluminum-garnet laser. Lasers Med Sci 26:193–204

    Article  PubMed  Google Scholar 

  34. Franzen R, Esteves-Oliveira M, Meister J, Wallerang A, Vanweersch L, Lampert F, Gutknecht N (2009) Decontamination of deep dentin by means of erbium, chromium:yttrium-scandium-gallium-garnet laser irradiation. Lasers Med Sci 24:75–80

    Article  PubMed  Google Scholar 

  35. Arnabat J, Escribano C, Fenosa A, Vinuesa T, Gay-Escoda C, Berini L, Vinas M (2010) Bactericidal activity of erbium, chromium:yttrium-scandium-gallium-garnet laser in root canals. Lasers Med Sci 25:805–810

    Article  PubMed  Google Scholar 

  36. Benatti BB, César – Neto JB, Gonçalves PF, Sallum EA, Nociti FH Jr (2005) Smoking affects the self-healing capacity of periodontal tissues. A histological study in the rat. Eur J Oral Sci 113:400–403

    Article  PubMed  Google Scholar 

  37. Nogueira-Filho GR, Rosa BT, César-Neto JB, Tunes Uda R (2007) Low-and high-yield cigarette smoke inhalation potentiates bone loss during ligature-induced periodontitis. J Periodontol 78:730–735

    Article  Google Scholar 

  38. Schwarz F, Aoki A, Sculean A, Becker J (2009) The impact of laser application on periodontal and peri-implant wound healing. Periodontol 2000 51:79–108

    Article  PubMed  Google Scholar 

  39. Lee JH, Heo SJ, Koak JY, Kim SK, Lee SJ, Lee SH (2008) Cellular responses on anodized titanium discs after laser irradiation. Lasers Surg Med 40:738–742

    Article  PubMed  Google Scholar 

  40. Romanos G, Crespi R, Barone A, Covani U (2006) Osteoblast attachment on titanium disks after laser irradiation. Int J Oral Maxillofac Implants 21:232–236

    PubMed  Google Scholar 

  41. Matarese G, Isola G, Anastasi GP, Favaloro A, Milardi D, Vermiglio G, Vita G, Cordasco G, Cutroneo G (2012) Immunohistochemical analysis of TGF-beta1 and VEGF in gingival and periodontal tissues: a role of these biomarkers in the pathogenesis of scleroderma and periodontal disease. Int J Mol Med 30:502–508

    PubMed  Google Scholar 

  42. Cesar-Neto JB, Benatti BB, Sallum EA, Casati MZ, Nociti FH Jr (2006) The influence of cigarette smoke inhalation and its cessation on the tooth-supporting alveolar bone: a histometric study in rats. J Periodontal Res 41:118–123

    Article  PubMed  Google Scholar 

  43. Correa MG, Gomes Campos ML, Cesar-Neto JB, Casati MZ, Nociti FH, Sallum EA (2009) Histometric evaluation of bone around titanium implants with different surface treatments in rats exposed to cigarette smoke inhalation. Clin Oral Implants Res 20:588–593

    PubMed  Google Scholar 

Download references

Acknowledgments

This study was financially supported by the São Paulo Research Foundation (FAPESP—process number 2009/17639-9 e 2010/16661-8, São Paulo, Brazil) and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes).

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Araraquara Dental School—São Paulo University “Júlio de Mesquita Filho”, Araraquara, São Paulo, Brazil

    Chaine Pavone, Andressa Vilas Boas Nogueira, Guilherme José Pimentel Lopes de Oliveira, Cássio Rocha Scardueli, Paulo Sérgio Cerri, Elcio Marcantonio Jr. & Rosemary Adriana Chiérici Marcantonio

Authors
  1. Chaine Pavone
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Andressa Vilas Boas Nogueira
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Guilherme José Pimentel Lopes de Oliveira
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Cássio Rocha Scardueli
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Paulo Sérgio Cerri
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Elcio Marcantonio Jr.
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Rosemary Adriana Chiérici Marcantonio
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Rosemary Adriana Chiérici Marcantonio.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Pavone, C., Nogueira, A.V.B., de Oliveira, G.J.P.L. et al. Treatment of periodontal disease with an Er,Cr:YSGG laser in rats exposed to cigarette smoke inhalation. Lasers Med Sci 30, 2095–2103 (2015). https://doi.org/10.1007/s10103-015-1731-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10103-015-1731-8

Keywords

Search

Navigation