Skip to main content
SpringerLink
Log in

Effect of low-level laser therapy on types I and III collagen and inflammatory cells in rats with induced third-degree burns

  • Original Article
  • Published:
Lasers in Medical Science Aims and scope Submit manuscript

Abstract

Low-level laser therapy (LLLT) has been increasingly used to accelerate wound healing in third-degree burns. This study investigated the effects of lasers on the tissue repair process of third-degree burns. Burns were produced on the backs of male Wistar rats. The animals were divided into four groups (n = 12): control, injury, LLLT 3 J/cm2, and LLLT 4 J/cm2. Each group was further divided into two subgroups; the rats in one subgroup were killed on day 8 and those in the other, on day 16 after injury. The animals in LLLT 3 J/cm2 and LLLT 4 J/cm2 were irradiated 1 h after injury, and irradiation was repeated every 48 h. Laser (660 nm, 35 mW) treatment at fluences of 3 and 4 J/cm2 were used. After killing the rats, tissue fragments from the burnt area were removed for histological analysis. The LLLT-treated groups showed a significant decrease (p <0.05) in the number of inflammatory cells and increased collagen deposition compared to the injury group. Laser irradiation (both 3 and 4 J/cm2) resulted in reduction in the inflammatory process and improved collagen deposition, thereby ameliorating the healing of third-degree burns.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Potokar TS, Prowse S, Whitaker IS (2008) A global overview of burns research highlights the need for forming networks with the developing world. Burns 34:3–5

    Article  PubMed  Google Scholar 

  2. Maurer M, Theoharides T, Granstein RD, Bischoff SC, Bienenstock J, Henz B et al (2003) What is the physiological function of mast cells? Exp Dermatol 12:886–910

    Article  CAS  PubMed  Google Scholar 

  3. Clark RAF (1993) Biology of dermal wound repair. Dermatol Clin 11:647–666

    CAS  PubMed  Google Scholar 

  4. Hawkins D, Houreld N, Abrahamse H (2005) Low level laser therapy (LLLT) as an effective therapeutic modality for delayed wound healing. Ann N Y Acad Sci 1056:486–493

    Article  CAS  PubMed  Google Scholar 

  5. Lyons RF, Abergel RP, White RA, Dwyer RM, Castel JC, Uitto J (1987) Biostimulation of wound healing in vivo by a helium-neon laser. Ann Plast Surg 18:47–50

    Article  CAS  PubMed  Google Scholar 

  6. Kana JS, Hutschenreiter G, Haina D, Waidelich W (1981) Effect of low-power density laser radiation on healing of open skin wounds in rats. Arch Surg 116(3):293–296

    Article  CAS  PubMed  Google Scholar 

  7. Mester E, Jaszsagi-Nagi E (1971) The effect of laser radiation on wound healing and collagen synthesis. Studia Biophys 35:227–230

    Google Scholar 

  8. Tredget EE, Ming YY (1992) The metabolic effects of thermal injury. World J Surg 16:68–79

    Article  CAS  PubMed  Google Scholar 

  9. Medrado ARAP, Pugliese LS, Reis SRA, Andrade ZA (2003) Influence of low level laser therapy on wound healing and its biological action upon myofibroblasts. Laser in Surg and Med 32(3):239–244

    Article  Google Scholar 

  10. Rochkind S, Rousso M, Nissan M et al (1989) Systemic effects of low-power irradiation on the peripheral and central nervous system, cutaneous wounds, and burns. Lasers Surg Med 9:174–182

    Article  CAS  PubMed  Google Scholar 

  11. Rabelo SB, Villaverde AB, Nicolau RA, Salgado MAC, Melo MS, Pacheco MT (2006) Comparison between wound healing in induced diabetic and nondiabetic rats after low-level laser therapy. Photomedicine and Laser Surgery 24(4):474–479. doi:10.1089/pho.2006.24.474

    Article  PubMed  Google Scholar 

  12. Meireles GCS, Santos JN, Chagas PO, Moura AP, Pinheiro ALB (2008) Effectiveness of laser photobiomodulation at 660 or 780 nm on the repair of third-degree burns in diabetic rats. Photomed Laser Surg 26(1):47–53

    Article  PubMed  Google Scholar 

  13. Rocha Júnior AM, Oliveira RG, Farias RE, Andrade LCR, Aarestrup FM (2006) Modulação da proliferação fibroblástica e da resposta inflamatória pela terapia a laser de baixa intensidade no processo de reparo tecidual (Modulation of fibroblast proliferation and inflammatory response by the laser therapy of low intensity in the process of tissue repair). An Bras Dermatol 81(2):150–156

    Article  Google Scholar 

  14. Dantas MDM, Cavalcante DRR, Araújo FEN, Barretto SR, Aciole GTS, Pinheiro ALB, Ribeiro MAG, Lima-Verde IB, Melo CM, Cardoso JC, Albuquerque Júnior RLC (2011) Improvement of dermal burn healing by combining sodium alginate/chitosan-based films and low level laser therapy. J Photochem and Photobiol B: Biol 105:51–59

    Article  CAS  Google Scholar 

  15. Garcia VG, Lima MA, Okamoto T, Milanezi LA, Gualberto EC, Fernandes LA, Almeida LM, Theodoro LH (2010) Effect of photodynamic therapy on the healing of cutaneous third-degree-burn: histological study in rats. Lasers Med Sci 25:221–228

    Article  PubMed  Google Scholar 

  16. Medrado AP, Soares AP, Santos ET, Reis SR, Andrade ZA (2009) Influence of laser photobiomodulation upon connective tissue remodeling during wound healing. J Photochem Photobiol B 92:144–152

    Article  Google Scholar 

  17. Ribeiro MAG, Albuquerque RLC, Barreto ALS, Oliveira VGM, Santos TB, Dantas CDF (2009) Morphological analysis of second-intention wound healing in rats submitted to 16 J/cm2 lambda 660-nm laser irradiation. Indian J Dent Res 20(3):390. doi:10.4103/0970-9290.57360

    Google Scholar 

  18. Pereira AN, Eduardo CDEP, Matson E, Marques MM (2002) Effect of low-power laser irradiation on cell growth and procollagen synthesis of cultured fibroblasts. Lasers Surg Med 31:263–267

    Article  PubMed  Google Scholar 

  19. Pereira MCMC, Pinho CB, Medrado ARP, Andrade ZA, Reis SRA (2010) Influence of 670 nm low-level laser therapy on mast cells and vascular response of cutaneous injuries. J Photochem Photobiol B 98:188–192

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Health Sciences Center, Chapecó University (UNOCHAPECÓ), Av. Sen. Atílio Fontana, Chapecó, SC, Brazil

    Franciane B. Fiório

  2. Postgraduate Program in Rehabilitation Sciences, Nove de Julho University (UNINOVE), Rua Vergueiro, 235, São Paulo, São Paulo, 01504-001, Brazil

    Franciane B. Fiório, Regiane Albertini, Ernesto Cesar Pinto Leal-Junior & Paulo de Tarso Camillo de Carvalho

  3. Postgraduate Program in Biophotonics Applied to Health Sciences, Nove de Julho University (UNINOVE), São Paulo, São Paulo, Brazil

    Regiane Albertini, Ernesto Cesar Pinto Leal-Junior & Paulo de Tarso Camillo de Carvalho

Authors
  1. Franciane B. Fiório
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Regiane Albertini
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ernesto Cesar Pinto Leal-Junior
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Paulo de Tarso Camillo de Carvalho
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Paulo de Tarso Camillo de Carvalho.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fiório, F.B., Albertini, R., Leal-Junior, E.C.P. et al. Effect of low-level laser therapy on types I and III collagen and inflammatory cells in rats with induced third-degree burns. Lasers Med Sci 29, 313–319 (2014). https://doi.org/10.1007/s10103-013-1341-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10103-013-1341-2

Keywords

Search

Navigation