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Low-power laser in increasing doses improve wound healing process in rats

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Abstract

Low-power laser has been studied and applied as an auxiliary tool in wound healing. However, as it is a therapy with several variables to be controlled, there is great difficulty in establishing protocols and comparing its efficacy. Therefore, the objective of this study was to evaluate the effects of the use of low-power laser in fixed and crescent doses in the healing of skin wounds in rats. Seventy-five male Wistar rats were divided into three groups: G1 with animals that did not receive laser radiation; G2 with animals treated with fixed dose of 3 J/cm2 laser; G3 with animals treated with laser in increasing doses of 1 J/cm2, 3 J/cm2, 5 J/cm2. Macroscopic and histological analysis were performed. The lowest intensity of PMN was observed in the irradiated groups and G3 had lower intensity of this infiltrate compared to G1 and G2 (p <0.05). On the seventh day of injury, PMN infiltrate decreased in all groups, especially in G3 (p<0.05). It was observed that G2 had more blood vessels than G1 and G3 after 7 days of wound creation (p ˂ 0.05). Collagen quantification showed that laser-treated groups have increased collagen deposition. Different responses in the wound healing process were observed comparing G2 and G3 groups. The fluence of 1J/cm2 presented better results in the anti-inflammatory action than 3 J/cm2, although G3 presented the greatest amount of total collagen after ten days of treatment.

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Acknowledgements

We would to thank Centro Multiusuário de Produção e Experimentaçao Animal (CMPEA) of the Instituto de Patologia Tropical e Saúde Pública (IPTSP) of the Universidade Federal de Goias (UFG) and Histotechnics and Innovation Laboratory (LHIn)/IPTSP/UFG by their for providing equipment and input materials.

Funding

The author Leiny Paula de Oliveira received personal financial support from her master’s degree scholarship sponsored by Higher Education Personnel Improvement Coordination (CAPES), a foundation linked to the Ministry of Education of Brazil.

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Authors and Affiliations

  1. Programa de Pós Graduação em Ciência Animal da Escola de Veterinaria e Zootecnia, Universidade Federal de Goiás (UFG), Goiânia, GO, Brazil

    Leiny Paula de Oliveira, Liliana Borges de Menezes, Marina Pacheco Miguel & Valcinir Aloisio Scalla Vulcani

  2. Instituto de Patologia Tropical e Saúde Pública (IPTSP), Universidade Federal de Goiás (UFG), Goiânia, GO, Brazil

    Angelica de Lima Chagas, Tainara Ribeiro de Souza, Isabela Rodrigues Araújo, Liliana Borges de Menezes & Marina Pacheco Miguel

  3. Medicina veterinária, Unidade Acadêmica de Ciências Agrárias, Universidade Federal de Jataí (UFJ), Jataí, GO, Brazil

    Valcinir Aloisio Scalla Vulcani

Authors
  1. Leiny Paula de Oliveira
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  2. Angelica de Lima Chagas
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  3. Tainara Ribeiro de Souza
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  4. Isabela Rodrigues Araújo
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  5. Liliana Borges de Menezes
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  7. Valcinir Aloisio Scalla Vulcani
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Contributions

Leiny Paula de Oliveira: bibliographic review, animals’ management, performing the procedure of animals, treatment of animals, production of photographic material (macroscopic and microscopic), assistance in histological procedures (cleavage and microtomy), writing of the work, textual formatting. Angelica de Lima Chagas: animals’ management, treatment of animals, assistance in the production of photographic material (macroscopic) and assistance in writing. Tainara Ribeiro de Souza: animals’ management; assistance in writing, text formatting, translation and final review. Isabela Rodrigues Araújo: animals’ management, treatment of animals, assistance in the production of photographic material (macroscopic) and assistance in writing. Liliana Borges de Menezes: orientation, writing of the work, scientific supervision, spelling, textual and grammatical correction, translation and final review. Marina Pacheco Miguel: orientation, writing of the work, scientific supervision, spelling, textual and grammatical correction, translation and final review. Valcinir Aloisio Scalla Vulcani: orientation, writing of the work, scientific supervision, spelling, textual and grammatical correction, translation and final review.

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Correspondence to Leiny Paula de Oliveira.

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de Oliveira, L.P., de Lima Chagas, A., de Souza, T.R. et al. Low-power laser in increasing doses improve wound healing process in rats. Lasers Med Sci 38, 60 (2023). https://doi.org/10.1007/s10103-023-03716-2

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