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Effect of photodynamic therapy on the healing of cutaneous third-degree-burn: histological study in rats

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Abstract

The aim of this study was to conduct a histological assessment of the effect of photodynamic therapy (PDT) on the repairing of third-degree-burn wounds made on the backs of rats with a heated scalpel. Ninety-six rats were divided into groups: G1, control (n = 24), cold scalpel; G2, burned, heated scalpel (n = 24); G3, low-level laser therapy (LLLT) (n = 24), on burns; and G4, photodynamic therapy (PDT) (n = 24), toluidine-O blue (100 µg/ml) and LLLT treatment on burns. The laser (685 nm) was applied in continuous mode, 50 mW, 4.5 J/cm2, contact mode at nine points (9 s/point). Eight animals in each group were killed at 3 days, 7 days or 14 days after surgery, and tissue specimens containing the whole wounded area were removed and processed for histological analysis; the results were statistically analyzed with Kruskal–Wallis and Dunn’s tests (P < 0.05). The results demonstrated significant differences between G2 and G3, and between G2 and G4, at both 3 days and 7 days, with regard to acute inflammation scores; G1 and G2 showed significant differences when compared with G4 at 3 days, with regard to neo-angiogenesis scores; G1 and G2 were statistically different from G3 and G4 at both 3 days and 7 days, with regard to re-epithelization scores; G2 showed statistically significant differences when compared with G3 and G4 with regard to collagen fiber scores at 7 days. LLLT and PDT acted as a biostimulating coadjuvant agent, balancing the undesirable effect of the burn on the wound healing process, acting mainly in the early healing stages, hastening inflammation and increasing collagen deposition.

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Authors and Affiliations

  1. Study and Research Group in Lasers in Dentistry, Araçatuba School of Dentistry, São Paulo State University (UNESP), Rua José Bonifácio, 1193, 16015-500, Araçatuba, São Paulo, Brazil

    Valdir Gouveia Garcia, Tetuo Okamoto, Luís Alberto Milanezi, Erivan Clementino Gualberto Júnior, Leandro Araújo Fernandes & Juliano Milanezi de Almeida

  2. Marilia University (UNIMAR), Marilia, São Paulo, Brazil

    Marcos Alcântara de Lima, Tetuo Okamoto & Luís Alberto Milanezi

  3. Barretos Educational Foundation (FEB), Barretos School of Dentistry, Barretos, São Paulo, Brazil

    Letícia Helena Theodoro

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  1. Valdir Gouveia Garcia
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  2. Marcos Alcântara de Lima
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  8. Letícia Helena Theodoro
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Correspondence to Valdir Gouveia Garcia.

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Garcia, V.G., de Lima, M.A., Okamoto, T. et al. Effect of photodynamic therapy on the healing of cutaneous third-degree-burn: histological study in rats. Lasers Med Sci 25, 221–228 (2010). https://doi.org/10.1007/s10103-009-0694-z

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  • DOI: https://doi.org/10.1007/s10103-009-0694-z

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