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Evaluation of dentinal tubule occlusion and pulp tissue response after using 980-nm diode laser for dentin hypersensitivity treatment

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Abstract

Objectives

To evaluate the effectiveness of the 980-nm diode laser for dentinal tubule occlusion, measure the intrapulpal temperature, and investigate the dental pulp response.

Materials and methods

The dentinal samples were randomly divided into G1–G7 groups: control; 980-nm laser irradiation (0.5 W, 10 s; 0.5 W, 10 s × 2; 0.8 W, 10 s; 0.8 W, 10 s × 2; 1.0 W, 10 s; 1.0 W, 10 s × 2). The dentin discs were applied for laser irradiation and analyzed by scanning electron microscopy (SEM). The intrapulpal temperature was measured on the 1.0-mm and 2.0-mm thickness samples, and then divided into G2–G7 groups according to laser irradiation. Moreover, forty Sprague Dawley rats were randomly divided into the laser-irradiated group (euthanized at 1, 7, and 14 days after irradiation) and the control group (non-irradiated). qRT-PCR, histomorphology, and immunohistochemistry analysis were employed to evaluate the response of dental pulp.

Results

SEM indicated the occluding ratio of dentinal tubules in the G5 (0.8 W, 10 s × 2) and G7 (1.0 W, 10 s × 2) were significantly higher than the other groups (p < 0.05). The maximum intrapulpal temperature rises in the G5 were lower than the standard line (5.5 ℃). qRT-PCR showed that the mRNA expression level of TNF-α and HSP-70 upregulated significantly at 1 day (p < 0.05). Histomorphology and immunohistochemistry analysis showed that, compared with the control group, the inflammatory reaction was slightly higher at the 1 and 7 days (p < 0.05) and decreased to the normal levels at 14 days (p > 0.05).

Conclusions

A 980-nm laser at a power of 0.8 W with 10 s × 2 defines the best treatment for dentin hypersensitivity in terms of compromise between the efficacy of the treatment and the safety of the pulp.

Clinical relevance

The 980-nm laser is an effective option for treating dentin sensitivity. However, we need to ensure the safety of the pulp during laser irradiation.

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Data availability

Data are available from the corresponding author.

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Funding

The work was supported by the National Natural Science Foundation of China (No. 81870798 and 82170927) and the National Science Basic Research Plan in Shaanxi Province of China (2020JM-414).

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Author notes

  1. Yulin Dong and Dandan Pei contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong University, Xi’an, 710004, China

    Yuchen Meng, Fan Huang, Silin Wang, Xin Huang, Yi Lu, Yuncong Li, Yulin Dong & Dandan Pei

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  1. Yuchen Meng
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  2. Fan Huang
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  3. Silin Wang
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  4. Xin Huang
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Contributions

Yuchen Meng: conceptualization, methodology, formal analysis, data curation, writing—original draft preparation, writing—review and editing, visualization. Fan Huang: methodology, validation, writing—review and editing. Silin Wang: investigation, writing—review and editing. Xin Huang: investigation, writing—review and editing. Yi Lu: investigation, writing—review and editing. Yuncong Li: methodology, investigation. Yulin Dong: conceptualization, investigation, writing—review and editing, supervision, project administration. Dandan Pei: conceptualization, resources, writing—review and editing, supervision, project administration.

Corresponding authors

Correspondence to Yulin Dong or Dandan Pei.

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Ethical approval

This study was approved by Ethics Committee of School and Hospital of Stomatology, Xi’an Jiaotong University (approved number xjkqll [2018]030), and Biomedical Ethics Committee of Xi’an Jiaotong University Health Science Center (approved number 20221440). The procedures were conducted in compliance with all ethical standards of the Declaration of Helsinki.

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Human teeth were used in this study after obtaining informed consent.

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Meng, Y., Huang, F., Wang, S. et al. Evaluation of dentinal tubule occlusion and pulp tissue response after using 980-nm diode laser for dentin hypersensitivity treatment. Clin Oral Invest 27, 4843–4854 (2023). https://doi.org/10.1007/s00784-023-05114-y

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