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Dentin sealing and antibacterial effects of silver-doped bioactive glass/mesoporous silica nanocomposite: an in vitro study

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Abstract

Objectives

To synthesize a silver-doped bioactive glass/mesoporous silica nanoparticle (Ag-BGN@MSN), as well as to investigate its effects on dentinal tubule occlusion, microtensile bond strength (MTBS), and antibacterial activity.

Materials and methods

Ag-BGN@MSN was synthesized using a modified “quick alkali-mediated sol-gel” method. Demineralized tooth disc models were made and divided into four groups; the following treatments were then applied: group 1—no treatment, group 2—bioglass, group 3—MSN, group 4—Ag-BGN@MSN. Next, four discs were selected from each group and soaked into 6 wt% citric acid to test acid-resistant stability. Dentinal tubule occlusion, as well as the occlusion ratio, was observed using field-emission scanning electron microscopy. The MTBS was also measured to evaluate the desensitizing effect of the treatments. Cytotoxicity was examined using the MTT assay. Antibacterial activity was detected against Lactobacillus casei, and ion dissolution was evaluated using inductively coupled plasma optical emission spectrometry.

Results

Ag-BGN@MSN effectively occluded the dentinal tubule and formed a membrane-like layer. After the acid challenge, Ag-BGN@MSN had the highest rate of dentinal tubule occlusion. There were no significant differences in MTBS among the four groups (P > 0.05). All concentrations of Ag-BGN@MSN used had a relative cell viability above 72%.

Conclusions

Ag-BGN@MSN was successfully fabricated using a modified sol-gel method. The Ag-BGN@MSN biocomposite effectively occluded dentinal with acid-resistant stability, did not decrease bond strength in self-etch adhesive system, had low cytotoxicity, and antibacterial effect.

Clininal relevance

Dentinal tubule sealing induced by Ag-BGN@MSN biocomposite with antibacterial effect is likely to increase long-term stability in DH.

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Funding

This study was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (NRF-2015R1C1A1A01051832).

Author information

Author notes

  1. Yong Hoon Kwon and Yong-Il Kim contributed equally to this article.

Authors and Affiliations

  1. Department of Orthodontics, Dental Research Institute, Pusan National University Dental Hospital, Geumoro 20, Mulgeum, Yangsan, 50612, Republic of Korea

    Jae-Hyun Jung & Yong-Il Kim

  2. R&D Center, Upex. Med Co., Ltd., Hagui-ro 282, Dongan-gu, Anyang, Gyeonggi-do, 14056, Republic of Korea

    Dong-Hyun Kim

  3. School of Materials Science and Engineering, Pusan National University, Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan, 46241, Republic of Korea

    Kyung-Hyeon Yoo & Seog-Young Yoon

  4. Department of Oral Physiology, School of Dentistry, Pusan National University, Busandaehak-ro 49, Mulgeum, Yangsan, 50612, Republic of Korea

    Yeon Kim & Moon-Kyoung Bae

  5. Department of Oral Microbiology, School of Dentistry, Pusan National University, Busandaehak-ro 49, Mulgeum, Yangsan, 50612, Republic of Korea

    Jin Chung

  6. Department of Orthodontics, School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27516, USA

    Ching-Chang Ko

  7. Department of Dental Materials, Pusan National University, Busandaehak-ro 49, Mulgeum, Yangsan, 50612, Republic of Korea

    Yong Hoon Kwon

  8. Institute of Translational Dental Sciences, School of Dentistry, Pusan National University, Busandaehak-ro 49, Mulgeum, Yangsan, 50612, Republic of Korea

    Yong-Il Kim

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  1. Jae-Hyun Jung
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  7. Jin Chung
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Corresponding authors

Correspondence to Yong Hoon Kwon or Yong-Il Kim.

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Conflict of interest

YI Kim has received research grants from the National Research Foundation of Korea (NRF) for this study. All authors declare that they have no competing interest.

Ethical approval

The study was approved by the Ethics Committee of Pusan National University Dental Hospital (PNUDH-2016-033).

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Informed consent was obtained from all individual participants included in the study.

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Jung, JH., Kim, DH., Yoo, KH. et al. Dentin sealing and antibacterial effects of silver-doped bioactive glass/mesoporous silica nanocomposite: an in vitro study. Clin Oral Invest 23, 253–266 (2019). https://doi.org/10.1007/s00784-018-2432-z

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  • DOI: https://doi.org/10.1007/s00784-018-2432-z

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