Skip to main content

Advertisement

SpringerLink
Log in

Complete genomic sequence and organization of a novel mycovirus from Phoma matteuccicola strain LG915

  • Annotated Sequence Record
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

The complete genome of a double-stranded RNA (dsRNA) mycovirus, Phoma matteuccicola partitivirus 1 (PmPV1) was sequenced. It consists of two dsRNA segments, 1664 bp (dsRNA-1) and 1383 bp (dsRNA-2) in length, each containing a single open reading frame (ORF) potentially encoding a 46.78-kDa protein and a 40.92-kDa protein, respectively. dsRNA-1 encodes a putative polypeptide with a conserved RNA-dependent RNA polymerase (RdRp) domain that shows sequence similarity to the corresponding proteins of partitiviruses. The protein encoded by dsRNA-2 has no significant similarity to the typical coat proteins (CPs) of partitiviruses, but structure analysis nevertheless suggested that it might function as a coat protein. Purified viral particles of PmPV1 were isometric and approximately 29 nm in diameter. Phylogenetic analysis showed that PmPV1 is closely related to members of the genus Gammapartitivirus within the family Partitiviridae but forms a separate branch with Colletotrichum acutatum RNA virus 1 and Ustilaginoidea virens partitivirus 2. This is the first report of the full-length nucleotide sequence of a novel virus of the genus Gammapartitivirus infecting P. matteuccicola strain LG915, the causal agent of leaf blight of Curcuma wenyujin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Ghabrial SA, Jiang DH, Nibert ML, Suzuki N (2015) 50-plus years of fungal viruses. Virology 479:356–368

    Article  CAS  PubMed  Google Scholar 

  2. Zhong J, Zhu JZ, Lei XH, Chen D, Zhu HJ, Gao BD (2014) Complete genome sequence and organization of a novel virus from the rice false smut fungus Ustilaginoidea virens. Arch Virol 48:329–333

    CAS  Google Scholar 

  3. Zheng F, Ma R, Xu G, Zheng FQ, Ding XF, Xie CP (2018) Leaf blight on Curcuma wenyujin caused by Phoma matteucciicola in China. Plant Dis 102:2042

    Article  Google Scholar 

  4. Morris TJ, Dodds JA (1979) Isolation and analysis of double stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69:854–858

    Article  CAS  Google Scholar 

  5. Zheng L, Liu HQ, Zhang ML, Cao X, Zhou EX (2013) The complete genomic sequence of a novel mycovirus from Rhizoctonia solani AG-1 IA strain B275. Arch Virol 158:1609–1612

    Article  CAS  PubMed  Google Scholar 

  6. Darissa O, Willingmann P, Adam G (2010) Optimized approaches for the sequence determination of double-stranded RNA templates. J Virol Methods 169:397–403

    Article  CAS  PubMed  Google Scholar 

  7. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Zhong J, Zhu JZ, Lei XH, Chen D, Zhu HJ, Gao BD (2014) Complete genome sequence and organization of a novel virus from the rice false smut fungus Ustilaginoidea virens. Virus Genes 48:329–333

    Article  CAS  PubMed  Google Scholar 

  10. Zhong J, Chen D, Lei XH, Zhu HJ, Zhu JZ, Gao BD (2014) Detection and characterization of a novel Gammapartitivirus in the phytopathogenic fungus Colletotrichum acutatum strain HNZJ001. Virus Res 190:104–109

    Article  CAS  PubMed  Google Scholar 

Download references

Funding

This study was financially supported by Hainan Province Key R&D Project (ZDYF2019143), the Scientific Research Foundation for Advanced Talents, Hainan University (no. KYQD(ZR)1873), Hainan Major Research Found of Science and Technology (no. ZDKJ201817), and the National Natural Science Foundation of China (no. 31701732).

Author information

Authors and Affiliations

  1. College of Plant Protection, Hainan University/Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, Haikou, 570228, People’s Republic of China

    Fan Zheng, Gang Xu, Jia Zhou, Changping Xie, Hongguang Cui, Weiguo Miao & Li Zheng

  2. State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, People’s Republic of China

    Zhensheng Kang

Authors
  1. Fan Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gang Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jia Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Changping Xie
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hongguang Cui
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Weiguo Miao
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Zhensheng Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Li Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Li Zheng.

Ethics declarations

Conflict of interest

All authors declare no conflict of interest.

Ethical approval

This article does not contain any studies with animals or human participants performed by any of the authors.

Additional information

Handling Editor: Robert H. A. Coutts.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zheng, F., Xu, G., Zhou, J. et al. Complete genomic sequence and organization of a novel mycovirus from Phoma matteuccicola strain LG915. Arch Virol 164, 2209–2213 (2019). https://doi.org/10.1007/s00705-019-04314-w

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-019-04314-w

Search

Navigation