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Complete genome sequence of a novel mycovirus from Phoma matteucciicola

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Abstract

The complete genome sequence of a novel mycovirus, Phoma matteucciicola RNA virus 1 (PmRV1), derived from Phoma matteucciicola strain LG-01, was sequenced and analyzed. The complete cDNA sequence of PmRV1 is 3432 bp in length with a GC content of 57.17%. The genome of PmRV1 contains two putative open reading frames (ORFs): ORF1 and ORF2. ORF1 encodes a hypothetical protein with significant similarity to a protein encoded by Periconia macrospinosa ambiguivirus 1 (PmAV1). ORF2 encodes a protein of 491 amino acids with a conserved RNA-dependent RNA polymerase (RdRp) domain. Additionally, the triad within domain III has an asparagine (GDN) instead of the nearly universally conserved aspartic acid (GDD). RdRp phylogeny showed that PmRV1 grouped together with PmAV1 as a sister branch of a new member of the recently proposed family of mycotombus-like viruses. This is first report of the complete sequence of a novel mycovirus, PmRV1, infecting Phoma matteucciicola strain LG-01, the causal agent of leaf blight of Curcuma wenyujin.

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References

  1. Ghabrial SA, Castón JR, Jiang D, Nibert ML, Suzuki N (2015) 50-plus years of fungal viruses. Virology 356:356–368

    Article  Google Scholar 

  2. Rosseto P, Costa AT, Polonio JC, da Silva AA, Pamphile JA, Azevedo JL (2016) Investigation of mycoviruses in endophytic and phytopathogenic strains of Colletotrichum from different hosts. Genet Mol Res 15:15017651

    Article  CAS  Google Scholar 

  3. Xie J, Jiang D (2014) New insights into mycoviruses and exploration for the biological control of crop fungal diseases. Annu Rev Phytopathol 52:45–68

    Article  CAS  Google Scholar 

  4. Donaire L, Pagán I, Ayllón MA (2016) Characterization of Botrytis cinerea negative-stranded RNA virus 1, a new mycovirus related to plant viruses, and a reconstruction of host pattern evolution in negative-sense ssRNA viruses. Virology 499:212–218

    Article  CAS  Google Scholar 

  5. Yu X, Li B, Fu Y, Jiang D, Ghabrial SA, Li G, Peng Y, Xie J, Cheng J, Huang J, Yi X (2010) A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus. Proc Natl Acad Sci USA 107:8387–8392

    Article  CAS  Google Scholar 

  6. Yu X, Li B, Fu Y, Xie J, Cheng J, Ghabrial SA, Li G, Yi X, Jiang D (2013) Extracellular transmission of a DNA mycovirus and its use as a natural fungicide. Proc Natl Acad Sci USA 110:1452–1457

    Article  CAS  Google Scholar 

  7. Li P, Wang S, Zhang L, Qiu D, Zhou X, Guo L (2020) A tripartite ssDNA mycovirus from a plant pathogenic fungus is infectious as cloned DNA and purified virions. Sci Adv 6:eaay9634

    Article  CAS  Google Scholar 

  8. Li Q, Huang W, Hai D, Wang Y, Xie J, Wang M (2020) The complete genome sequence of a novel hypovirus infecting Bipolaris oryzae. Arch Virol 165:1027–1031

    Article  CAS  Google Scholar 

  9. Muhammad H, Xie J, Wu S, Shahzeen M, Dan Z, Abdoulaye AH, Wang Q, Cheng J, Fu Y, Jiang D (2018) A novel deltaflexivirus that infects the plant fungal pathogen, Sclerotinia sclerotiorum, can be transmitted among host vegetative incompatiblestrains. Viruses 10:295

    Article  Google Scholar 

  10. Li K, Zheng D, Cheng J, Chen T, Xie J (2015) Characterization of a novel Sclerotinia sclerotiorum RNA virus as the prototype of a new proposed family within the order Tymovirales. Virus Res 219:92–99

    Article  Google Scholar 

  11. Gilbert KB, Holcomb EE, Allscheid RL, Carrington JC (2019) Hiding in plain sight: New virus genomes discovered via a systematic analysis of fungal public transcriptomes. PLoS ONE 14:e0219207

    Article  CAS  Google Scholar 

  12. Ai Y, Zhong J, Chen C, Zhu H, Gao B (2016) A novel single-stranded RNA virus isolated from the rice-pathogenic fungus Magnaporthe oryzae with similarity to members of the family Tombusviridae. Arch Virol 161:725–729

    Article  CAS  Google Scholar 

  13. Zheng F, Ma R, Xu G, Zheng F, Ding X, Xie C (2018) Leaf blight on Curcuma wenyujin caused by Phoma matteucciicola in China. Plant Dis 102:2042

    Article  Google Scholar 

  14. Zheng F, Xu G, Zhou J, Xie C, Cui H, Miao W, Kang Z, Zheng L (2019) Complete genomic sequence and organization of a novel mycovirus from Phoma matteuccicola strain LG915. Arch Virol 164:2209–2213

    Article  CAS  Google Scholar 

  15. Zhou J, Wang Y, Liang X, Xie C, Liu W, Miao W, Kang Z, Zheng L (2020) Molecular characterization of a novel Ourmia-Like virus infecting Phoma matteucciicola. Viruses 12:231

    Article  CAS  Google Scholar 

  16. Morris TJ, Dodds JA (1979) Isolation and analysis of double-Stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69:854–858

    Article  CAS  Google Scholar 

  17. Vázquez AL, Alonso JM, Parra F (2000) Mutation analysis of the GDD sequence motif of a calicivirus RNAdependent RNA polymerase. J Virol 74:3888–3891

    Article  Google Scholar 

Download references

Funding

This study was financially supported by Hainan Province Key R&D Project (ZDYF2019143), the Scientific Research Foundation for Advanced Talents, Hainan University (no. KYQD(ZR)1873), Hainan Major Research Found of Science and Technology (no. ZDKJ201817), and the National Natural Science Foundation of China (no. 31701732).

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Author notes

  1. Jia Zhou and Xiaochuan Hu contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Green Prevention and Control of Tropical Plant Disease and Pests, Ministry of Education and College of Plant Protection, Hainan University, Haikou, 570228, Hainan, China

    Jia Zhou, Xiaochuan Hu, Yuhua Wang, Changping Xie & Li Zheng

  2. State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Xiaofei Liang

Authors
  1. Jia Zhou
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  2. Xiaochuan Hu
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  3. Xiaofei Liang
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  4. Yuhua Wang
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  5. Changping Xie
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  6. Li Zheng
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Correspondence to Li Zheng.

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Zhou, J., Hu, X., Liang, X. et al. Complete genome sequence of a novel mycovirus from Phoma matteucciicola. Arch Virol 166, 317–320 (2021). https://doi.org/10.1007/s00705-020-04865-3

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  • DOI: https://doi.org/10.1007/s00705-020-04865-3

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