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The complete genomic sequence of a novel mycovirus from Rhizoctonia solani AG-1 IA strain B275

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Abstract

The complete genome of a novel mycovirus, Rhizoctonia solani dsRNA virus 1 (RsRV1) was sequenced and analyzed. It is composed of two dsRNA genome segments, 2379 bp and 1811 bp in length, which were referred to as RsRV1-1 and RsRV1-2, respectively. RsRV1-1 contains a single open reading frame (ORF1), which has a conserved RNA-dependent RNA polymerase (RdRp) domain, whereas RsRV1-2 contains a single ORF2, which might encode a multifunctional protein. The genome organization of RsRV1 is similar to that of members of the family Partitiviridae. However, phylogenetic analysis indicated that RsRV1 formed a distinct clade together with three other unclassified viruses, suggesting that RsRV1 may belong to a new family of dsRNA mycoviruses. This is the first report of the full-length nucleotide sequence of a novel dsRNA mycovirus, RsRV1, infecting R. solani AG-1 IA strain B275, the causal agent of rice sheath blight.

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Acknowledgments

The authors would like to thank Prof. Daohong Jiang and his Ph.D. students at Huazhong Agricultural University, Wuhan, Hubei, China, for their excellent technical assistance and helpful comments on the manuscript. This research was partly supported by a ‘Special Fund for Agro-scientific Research in the Public Interest’ (nyhyzx3-16) from the Ministry of Agriculture of China and by the NSFC grant (No. 31271994) from the National Natural Science Foundation of China awarded to Erxun Zhou.

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Authors and Affiliations

  1. Department of Plant Pathology, South China Agricultural University, Guangzhou, Guangdong, 510642, China

    Li Zheng, Meiling Zhang, Xiong Cao & Erxun Zhou

  2. Department of Plant Pathology, Northwest A & F University, Yangling, Shanxi, 712100, China

    Huiquan Liu

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  1. Li Zheng
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  2. Huiquan Liu
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  3. Meiling Zhang
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  4. Xiong Cao
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  5. Erxun Zhou
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Correspondence to Erxun Zhou.

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Zheng, L., Liu, H., Zhang, M. et al. The complete genomic sequence of a novel mycovirus from Rhizoctonia solani AG-1 IA strain B275. Arch Virol 158, 1609–1612 (2013). https://doi.org/10.1007/s00705-013-1637-3

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  • DOI: https://doi.org/10.1007/s00705-013-1637-3

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