Abstract
The fijivirus southern rice black-streaked dwarf virus (SRBSDV) causes one of the most serious viral diseases of rice in China and Vietnam. To better understand the molecular basis of SRBSDV infection, a yeast two-hybrid screen of a rice cDNA library was carried out using P8, a minor core protein of SRBSDV, as the bait. A rice Cys2His2-type zinc finger protein (OsZFP) was found to interact with SRBSDV P8. A strong interaction between SRBSDV P8 and OsZFP was then confirmed by pull-down assays, and bimolecular fluorescence complementation assays showed that the in vivo interaction was specifically localized in the nucleus of plant cells. Using a series of deletion mutants, it was shown that both the NTP-binding region of P8 and the first two zinc fingers of OsZFP were crucial for their interaction in plant cells. The localization in the nucleus and activation of transcription in yeast supports the notion that OsZFP is a transcription factor. SRBSDV P8 may play an important role in fijiviral infection and symptom development by interfering with the host transcription activity of OsZFP.
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Acknowledgements
This work was funded by National Science and Technology Support Program (2012BAD19B03), the National Natural Science Foundation of China (31601603), the China 973 Program (2010CB126203) and the Zhejiang Provincial Foundation for Natural Science (LQ14C140003 and Y3090657). We thank Professor M.J. Adams, Stevenage, UK for help in correcting the English of the manuscript.
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Li, J., Cai, NJ., Xue, J. et al. Interaction between southern rice black-streaked dwarf virus minor core protein P8 and a rice zinc finger transcription factor. Arch Virol 162, 1261–1273 (2017). https://doi.org/10.1007/s00705-017-3233-4
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DOI: https://doi.org/10.1007/s00705-017-3233-4