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A black-streaked dwarf disease on rice in China is caused by a novel fijivirus

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Abstract

An isolate of a plant reovirus causing severe stunting and dark leaf symptoms on rice from Guangdong, China, was similar in virion morphology and serologically related to rice black-streaked dwarf virus (RBSDV). The electrophoretic profiles of genome segments of the two viruses in agarose or polyacrylamide gel were indistinguishable. The four genome segments of the new isolate corresponding to RBSDV S7–S10 were amplified by ligation RT-PCR and sequenced. The size and organization of each genome segment was very similar to its counterparts in RBSDV, maize rough dwarf virus (MRDV), and mal de Rio Cuarto virus (MRCV). Sequence identity was greatest to RBSDV and MRDV (ranging from about 60–85% depending on the protein), but identities were always much lower than those between RBSDV and MRDV. These comparisons and phylogenetic analyses suggested that the virus represents a new species in genus Fijivirus group 2, tentatively named Rice black-streaked dwarf virus-2.

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Acknowledgments

This work was funded by the China 863 program (2007AA10Z414) and 973 Program (2006CB708209), and the Zhejiang Provincial Foundation for Natural Science (Z305165). We thank Mr Xu Zhao-Wei, Station of Plant Protection of Yangxi County, Guangdong Province, for providing useful help during collection of virus-infected rice plants. Rothamsted Research receives grant-aided support from the Biotechnology and Biological Sciences Research Council of the United Kingdom.

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Authors and Affiliations

  1. Virology Laboratory, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China

    Heng-Mu Zhang, Jian Yang & Jian-Ping Chen

  2. Department of Plant Pathology and Microbiology, Rothamsted Research, Harpenden, Herts, AL5 2JQ, UK

    M. J. Adams

  3. Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People’s Republic of China

    Heng-Mu Zhang & Jian-Ping Chen

Authors
  1. Heng-Mu Zhang
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  2. Jian Yang
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  3. Jian-Ping Chen
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  4. M. J. Adams
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Correspondence to Heng-Mu Zhang or Jian-Ping Chen.

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Zhang, HM., Yang, J., Chen, JP. et al. A black-streaked dwarf disease on rice in China is caused by a novel fijivirus. Arch Virol 153, 1893–1898 (2008). https://doi.org/10.1007/s00705-008-0209-4

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  • DOI: https://doi.org/10.1007/s00705-008-0209-4

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