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Electrochemical determination of Salmonella typhimurium by using aptamer-loaded gold nanoparticles and a composite prepared from a metal-organic framework (type UiO-67) and graphene

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Abstract

An aptamer based assay is described for the determination of Salmonella typhimurium (S.typhimurium). A metal-organic framework-graphene composite of type UiO-67/GR is used as the substrate, and an aptamer-gold nanoparticles-horseradish peroxidase (Apt-AuNP-HRP) conjugate the signal amplification probe. A phosphate-terminal and partially complementary DNA (cDNA) of the aptamer is covalently bound to UiO-67/GR via the chemical complexation between phosphate and Zr-OH groups of UiO-67, and then S. typhimurium and cDNA will compete for the binding sites. The binding of Apt-AuNP-HRP to S.typhimurium leads to the formation of strong conjugates. The unbound signal probes then attach to the surface of a glassy carbon electrode via hybridization with cDNA. This generates a large current response (best measured at a potential as low as −0.02 V vs. saturated calomel electrode) under the catalytic action of HRP on the H2O2-hydroquinone system. Under the optimal conditions, the differential pulse voltammetric signal decreases linearly in the 2 × 101 – 2 × 108 cfu·mL−1 S.typhimurium concentration range, with a lower detection limit of 5 cfu·mL−1 (based on S/N = 3). The method was successfully applied to the detection of S. typhimurium in spiked milk samples.

Schematic presentation of electrochemical determination of Salmonella typhimurium (S.typhimurium). A metal-organic framework (type UiO-67) and graphene (GR) composite were used as substrate, and gold nanoparticles carrying horseradish peroxidase (HRP) for signal amplification. HQ: hydroquinone; cDNA: complementary DNA of aptamer.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 21575042).

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Authors and Affiliations

  1. School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, People’s Republic of China

    Ge Dai, Zhi Li, Feifei Luo, Bo Chen & Qingjiang Wang

  2. College of Chemistry and Material Science, Shandong Agricultural University, Taian, 271018, Shandong, People’s Republic of China

    Shiyun Ai

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  1. Ge Dai
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Correspondence to Bo Chen or Qingjiang Wang.

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Dai, G., Li, Z., Luo, F. et al. Electrochemical determination of Salmonella typhimurium by using aptamer-loaded gold nanoparticles and a composite prepared from a metal-organic framework (type UiO-67) and graphene. Microchim Acta 186, 620 (2019). https://doi.org/10.1007/s00604-019-3724-y

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