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Impedimetric Salmonella aptasensor using a glassy carbon electrode modified with an electrodeposited composite consisting of reduced graphene oxide and carbon nanotubes

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Abstract

We describe a Salmonella biosensor that was obtained by electrochemical immobilization of a nanocomposite consisting of reduced graphene oxide (rGO) and carboxy-modified multi-walled carbon nanotubes (MWCNTs) directly on the surface of a glassy carbon electrode (GCE). An amino-modified aptamer specific for Salmonella was covalently bound to the rGO-MWCNT composite via amide bonds. The morphology of the rGO-MWCNT nanocomposite was characterized by transmission electron microscopy and scanning electron microscopy. Cyclic voltammetry and electrochemical impedance spectroscopy were used to monitor all steps during assembly. When exposed to samples containing Salmonella, the anti-Salmonella aptamer on the electrode captures its target. Hence, electron transfer is blocked, and this results in a large increase in impedance. Salmonella can be quantified by this aptasensor, typically operated at a working voltage of 0.2 V (vs. Ag/AgCl), in the range from 75 to 7.5 × 105 cfu⋅mL−1 and detection limit of 25 cfu⋅mL−1 (at an S/N of 3). The method is perceived to have a wide scope in that other bacteria may be detected by analogy to this approach and with very low limits of detection by applying respective analyte-specific aptamers.

The schematic view of the GCE surface modification and the detection of Salmonella

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Acknowledgments

This work was partly supported by the National S&T Support Program of China (2012BAK08B01, 2012BAD28B02), NSFC (21375049), the China Agriculture Research System Poultry-related Science and Technology Innovation Team of Peking (CARS-PSTP).

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Authors and Affiliations

  1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China

    Fei Jia, Ruitong Dai & Xingmin Li

  2. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China

    Fei Jia, Nuo Duan, Shijia Wu & Zhouping Wang

  3. Beijing Higher Institution Engineering Research Center of Animal Product, Beijing, China

    Xingmin Li

Authors
  1. Fei Jia
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  2. Nuo Duan
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  3. Shijia Wu
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  4. Ruitong Dai
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  5. Zhouping Wang
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  6. Xingmin Li
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Correspondence to Zhouping Wang or Xingmin Li.

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Jia, F., Duan, N., Wu, S. et al. Impedimetric Salmonella aptasensor using a glassy carbon electrode modified with an electrodeposited composite consisting of reduced graphene oxide and carbon nanotubes. Microchim Acta 183, 337–344 (2016). https://doi.org/10.1007/s00604-015-1649-7

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  • DOI: https://doi.org/10.1007/s00604-015-1649-7

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