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A fluorescent glucose bioassay based on the hydrogen peroxide-induced decomposition of a quencher system composed of MnO2 nanosheets and copper nanoclusters

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Abstract

The authors describe a fluorometric glucose assay that is based on the use of MnO2 nanosheets and copper nanoclusters (CuNCs) acting as nanoprobes. The CuNCs were synthesized by using bovine serum albumin as a template by chemical reduction of copper(II) sulfate. On addition of MnO2 nanosheets to a colloidal solution of CuNCs, the fluorescence of CuNCs (measured at excitation/emission wavelengths of 335/410 nm) is quenched. However, in the presence of enzymatically generated H2O2, the MnO2 nanosheets are reduced to form Mn(II) ions. As a result, fluorescence intensity recovers. The glucose assay is based on the enzymatic conversion of glucose by glucose oxidase to generate H2O2 and glucuronic acid. The calibration plot is linear in the 1 μM to 200 μM glucose concentration range, and the detection limit is 100 nM. The method was successfully applied to the determination of glucose in spiked human serum samples.

A sensitive fluorescent bioassay is reported for the detection of glucose based on the hydrogen peroxide-induced decomposition of a quencher system composed of MnO2 nanosheets and copper nanoclusters (CuNCs).

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Acknowledgements

This work was financially supported by National Natural Science Foundation of China (No. 21305119, 21375114), Nanhu Scholars Program for Young Scholars of XYNU, Plan For Scientific Innovation Talent of Henan Province.

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, 464000, People’s Republic of China

    Hai-Bo Wang, Ying Chen, Na Li & Yan-Ming Liu

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  1. Hai-Bo Wang
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  2. Ying Chen
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  3. Na Li
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  4. Yan-Ming Liu
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Correspondence to Hai-Bo Wang.

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Wang, HB., Chen, Y., Li, N. et al. A fluorescent glucose bioassay based on the hydrogen peroxide-induced decomposition of a quencher system composed of MnO2 nanosheets and copper nanoclusters. Microchim Acta 184, 515–523 (2017). https://doi.org/10.1007/s00604-016-2045-7

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  • DOI: https://doi.org/10.1007/s00604-016-2045-7

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