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Sensitive electrochemical sensor for hydrogen peroxide using Fe3O4 magnetic nanoparticles as a mimic for peroxidase

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Abstract

A sensor for hydrogen peroxide is described that is based on an indium tin oxide electrode modified with Fe3O4 magnetic nanoparticles which act as a mimic for the enzyme peroxidase and greatly improve the analytical performance of the sensor. The amperometric current is linearly related to the concentration of H2O2 in the range from 0.2 mM to 2 mM, the regression equation is y = -0.5–1.82x, the correlation coefficient is 0.998 (n = 3), and the detection limit is 0.01 mM (S/N = 3). The sensor exhibits favorable selectivity and excellent stability.

Using the peroxidase mimic property of Fe3O4 magnetic nanoparticles (MNPs), a sensitive electrochemical method with favorable analytical performance for the determination of hydrogen peroxide (H2O2) was developed.

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References

  1. Xu K, Huang JR, Ye ZZ, Ying YB, Li YB (2009) Recent development of nano-materials used in DNA biosensors. Sensors 9:5534–5557

    Article  CAS  Google Scholar 

  2. Bergemann C, Muller-Schulte D, Oster J, àBrassard L, Lubbe AS (1999) Magnetic ion-exchange nano- and microparticles for medical, biochemical and molecular biological applications. J Magn Magn Mater 194:45–52

    Article  CAS  Google Scholar 

  3. Brahler M, Georgieva R, Buske N, Muller A, Muller S, Pinkernelle J, Teichgraber U, Voigt A, Baumler H (2006) Magnetite-loaded carrier erythrocytes as contrast agents for magnetic resonance imaging. Nano Lett 6:2505–2509

    Article  CAS  Google Scholar 

  4. Yang HH, Zhang SQ, Chen XL, Zhuang ZX, Xu JG, Wang XR (2004) Magnetite-containing spherical silica nanoparticles for biocatalysis and bioseparations. Anal Chem 76:1316–1321

    Article  CAS  Google Scholar 

  5. Stevens PD, Fan J, Gardimalla HMR, Yen M, Gao Y (2005) Superparamagnetic nanoparticle-supported catalysis of suzuki cross-coupling reactions. Org Lett 7:2085–2088

    Article  CAS  Google Scholar 

  6. Hu A, Yee GT, Lin W (2005) Magnetically recoverable chiral catalysts immobilized on magnetite nanoparticles for asymmetric hydrogenation of aromatic ketones. J Am Chem Soc 127:12486–12487

    Article  CAS  Google Scholar 

  7. Tsang SC, Caps V, Paraskevas I, Chadwick D, Thompsett D (2004) Magnetically separable, carbon-supported nanocatalysts for the manufacture of fine chemicals. Angew Chem Int Ed 43:5645–5649

    Article  CAS  Google Scholar 

  8. Yoon TJ, Lee W, Oh YS, Lee JK (2003) Magnetic nanoparticles as a catalyst vehicle for simple and easy recyclingy. New J Chem 27:227–229

    Article  CAS  Google Scholar 

  9. Gao LZ, Zhuang J, Nie L, Zhang JB, Zhang Y, Gu N, Wang TH, Feng J, Yang DL, Perrett S, Yan XY (2007) Intrinsic peroxidase-like activity of ferromagnetic nanoparticles. Nat Nanotechnol 2:577–583

    Article  CAS  Google Scholar 

  10. Wei H, Wang EK (2008) Fe3O4 Magnetic nanoparticles as peroxidase mimetics and their applications in H2O2 and glucose detection. Anal Chem 80:2250–2254

    Article  CAS  Google Scholar 

  11. Zhang ZX, Wang ZJ, Wang XL, Yang XR (2010) Magnetic nanoparticle-linked colorimetric aptasensor for the detection of thrombin. Sens Actuators B Chem 147:428–433

    Article  Google Scholar 

  12. Schachl K, Alemu H, Kalcher K, Jezkova J, Svancara I, Vytras K (1997) Amperometric determination of hydrogen peroxide with a manganese dioxide-modified carbon paste electrode using flow injection analysis. Analyst 122:985–989

    Article  CAS  Google Scholar 

  13. Wang L, Wang EK (2004) A novel hydrogen peroxide sensor based on horseradish peroxidase immobilized on colloidal Au modified ITO electrode. Electrochem Commun 6:225–229

    Article  CAS  Google Scholar 

  14. Gao LZ, Wu JM, Lyle S, Gao D (2008) Magnetite nanoparticle-linked immunosorbent assay. J Phys Chem C 112:17357–17361

    Article  CAS  Google Scholar 

  15. Lawrie G, Keen I, Drew B, Grøndahl L (2007) Interactions between alginate and chitosan biopolymers characterized using FTIR and XPS. Biomacromolecules 8:2533–2541

    Article  CAS  Google Scholar 

  16. Kolhe P, Kannan RM (2003) Improvement in ductility of chitosan through blending and copolymerization with PEG: FTIR investigation of molecular interactions. Biomacromolecules 4:173–180

    Article  CAS  Google Scholar 

  17. Ge JP, Hu YX, Biasini M, Beyermann WP, Yin YD (2007) Superparamagnetic magnetite colloidal nanocrystal clusters. Angew Chem Int Ed 46:4342–4345

    Article  CAS  Google Scholar 

  18. Guo SJ, Dong SJ, Wang EK (2009) A general route to construct diverse multifunctional Fe3O4/metal hybrid nanoparticles. Chem Eur J 15:2416–2424

    Article  CAS  Google Scholar 

  19. Teng XW, Black D, Watkins NJ, Gao YL, Yang H (2003) Platinum-maghemite core-shell nanoparticles using a sequential synthesis. Nano Lett 3:261–264

    Article  CAS  Google Scholar 

  20. Wang LY, Luo J, Fan Q, Suzuki M, Suzuki IS, Engelhard MH, Lin YH, Kim N, Wang JQ, Zhong CJ (2005) Monodispersed core-shell Fe3O4@Au nanoparticles. J Phys Chem B 109:21593–21601

    Article  CAS  Google Scholar 

  21. Luo HX, Shi ZJ, Li NQ, Gu ZN, Zhuang QK (2001) Investigation of the electrochemical and electrocatalytic behavior of single-wall carbon nanotube film on a glassy carbon electrode. Anal Chem 73:915–920

    Article  CAS  Google Scholar 

  22. Miao YQ, Tian SN (2000) Amperometric hydrogen peroxide biosensor based on immobilization of peroxidase in chitosan matrix crosslinked with glutaraldehyde. Analyst 125:1591–1594

    Article  CAS  Google Scholar 

  23. Wang G, Xu JJ, Chen HY, Lu ZH (2003) Amperometric hydrogen peroxide biosensor with sol-gel/chitosan network-like film as immobilization matrix. Biosens Bioelectron 18:335–343

    Article  CAS  Google Scholar 

  24. Huang JS, Liu Y, Hou HQ, You TY (2008) Simultaneous electrochemical determination of dopamine, uric acid and ascorbic acid using palladium nanoparticle-loaded carbon nanofibers modified electrode. Biosens Bioelectron 24:632–637

    Article  CAS  Google Scholar 

  25. Liu Y, Huang JS, Hou HQ, You TY (2008) Simultaneous determination of dopamine, ascorbic acid and uric acid with electrospun carbon nanofibers modified electrode. Electrochem Commun 10:1431–1434

    Article  CAS  Google Scholar 

  26. Wang F, Hu SS (2009) Electrochemical sensors based on metal and semiconductor nanoparticles. Microchim Acta 165:1–22

    Article  CAS  Google Scholar 

  27. Li YH, Liu XY, Zeng XD, Liu Y, Liu XS, Wei WZ, Luo SL (2009) Nonenzymatic hydrogen peroxide sensor based on a Prussian Blue-modified carbon ionic liquid electrode. Microchim Acta 165:393–398

    Article  CAS  Google Scholar 

  28. Zeng JX, Wei WZ, Liu XY, Wang Y, Luo GM (2008) A simple method to fabricate a Prussian Blue nanoparticles/carbon nanotubes/poly(1,2-diaminobenzene) based glucose biosensor. Microchim Acta 160:261–267

    Article  CAS  Google Scholar 

  29. Zhang L, Zhai Y, Gao N, Wen D, Dong S (2008) Sensing H2O2 with layer-by-layer assembled Fe3O4-PDDA nanocomposite film. Electrochem Commun 10:1524–1526

    Article  CAS  Google Scholar 

  30. Peng FF, Zhang Y, Gu N (2008) Size-dependent peroxidase-like catalytic activity of Fe3O4 nanoparticles. Chin Chem Lett 19:730–733

    Article  CAS  Google Scholar 

  31. Liu Y, Chu ZY, Jin WQ (2009) A sensitivity-controlled hydrogen peroxide sensor based on self-assembled Prussian Blue modified electrode. Electrochem Commun 11:484–487

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 90713022), the National Key Basic Research Development Project of China (No. 2007CB714500) and the Project of Chinese Academy of Sciences (No.KJCX2-YW-H11).

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Authors and Affiliations

  1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Renmin Street 5625, Changchun, Jilin, 130022, China

    Zhanxia Zhang, Hui Zhu, Xiaolei Wang, Xiurong Yang & Xiurong Yang

  2. Graduate School of the Chinese Academy of Sciences, Beijing, 100039, China

    Zhanxia Zhang

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  1. Zhanxia Zhang
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  2. Hui Zhu
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  3. Xiaolei Wang
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  4. Xiurong Yang
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Correspondence to Xiurong Yang.

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Zhang, Z., Zhu, H., Wang, X. et al. Sensitive electrochemical sensor for hydrogen peroxide using Fe3O4 magnetic nanoparticles as a mimic for peroxidase. Microchim Acta 174, 183–189 (2011). https://doi.org/10.1007/s00604-011-0600-9

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  • DOI: https://doi.org/10.1007/s00604-011-0600-9

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