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Copper(II)-coated Fe3O4 nanoparticles as an efficient enzyme mimic for colorimetric detection of hydrogen peroxide

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Abstract

The authors describe the preparation of Cu(II)-coated Fe3O4) nanoparticles (NPs) that possess excellent peroxidase-like activity. The NPs were formed by chelation between Cu(II) ions and the oxygen functional groups of sodium ligninsulfonate. The morphology and structure of the NPs were characterized by scanning electron microscopy, transmission electron microscopy, X-ray powder diffraction, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy. The NPs have an average diameter of 220 nm. They are shown to be viable peroxidase mimics that can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine by hydrogen peroxide to produce a blue coloration. The findings were used to design a colorimetric assay that has a linear response in the 2.5 to 100 μM H2O2 concentration range and a 0.2 μM detection limit. The assay excels by its selectivity, high sensitivity, good selectivity, portability and cost efficiency.

Fe3O4-Cu2+ nanoparticles with excellent peroxidase-like activity were successfully prepared via a facile strategy, and then used to design a facile as well as sensitive colorimetric H2O2 sensor. The linear range and the detection limit were 2.5~100 μM and 0.212 μM.

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References

  1. Rhee SG (2006) Cell signaling. H2O2, a necessary evil for cell signaling. Science 312:1882–1883

    Article  Google Scholar 

  2. Li C, Wang S, Huang Y, Wen Q, Kan Y (2014) Photoluminescence properties of a novel cyclometalated iridium (III) complex with coumarin-boronate and its recognition of hydrogen peroxide. Dalton T 43(14):5595–5602

    Article  CAS  Google Scholar 

  3. Lee M, Patil V, Na Y, Lee D, Lim S, Yi G (2018) Highly stable, rapid colorimetric detection of carbaryl pesticides by azo coupling reaction with chemical pre-treatment. Sensors Actuat B-Chem 261:489–496

    Article  CAS  Google Scholar 

  4. Nasir M, Nawaz MH, Yaqub M, Hayat A, Rahim A (2017) An overview on enzyme-mimicking nanomaterials for use in electrochemical and optical assays. Microchim Acta 184(2):323–342

    Article  CAS  Google Scholar 

  5. Gao L, Zhuang J, Nie L, Zhang J, Zhang Y, Gu N, Wang T, Feng J, Yang D, Perrett S, Yan X (2007) Intrinsic peroxidase-like activity of ferromagnetic nanoparticles. Nat Nanotechnol 2(9):577–583

    Article  CAS  Google Scholar 

  6. Zhang Z, Wang Z, Wang X, Yang X (2010) Magnetic nanoparticle-linked colorimetric aptasensor for the detection of thrombin. Sensors Actuat B-Chem 147(2):428–433

    Article  CAS  Google Scholar 

  7. Safarik I, Safarikova M (2004) Magnetic techniques for the isolation and purification of proteins and peptides. Biomagn Res Technol 2:1–17

    Article  Google Scholar 

  8. Ito A, Hibino E, Kobayashi C, Terasaki C, Kagami H, Ueda M, Kobayashi T, Honda H (2005) Construction and delivery of tissue-engineered human retinal pigment epithelial cell sheets, using magnetite nanoparticles and magnetic force. Tissue Eng A 11:489–496

    Article  CAS  Google Scholar 

  9. Ding C, Yan Y, Xiang D, Zhang C, Xian Y (2016) Magnetic Fe3S4, nanoparticles with peroxidase-like activity, and their use in a photometric enzymatic glucose assay. Microchim Acta 183:625–631

    Article  CAS  Google Scholar 

  10. Lin T, Lin F, Lin J (2012) In vitro feasibility study of the use of a magnetic electrospun chitosan nanofiber composite for hyperthermia treatment of tumor cells. Acta Biomater 8:2704–2711

    Article  CAS  Google Scholar 

  11. Jiang Y, Jiang J, Gao Q, Ruan M, Yu H, Qi L (2008) A novel nanoscale catalyst system composed of nanosized Pd catalysts immobilized on Fe3O4@SiO2-PAMAM. Nanotechnol. 19:075714

    Article  Google Scholar 

  12. Wang J, Yang X, Li A, Cai X (2018) Preparation and characterization of multifunctional Fe3O4-coated Ag nanocomposites for catalytic reduction of 4-nitrophenol. Mater Lett 220:24–27

    Article  CAS  Google Scholar 

  13. Pham V, Kim D, Ko S (2017) Cu@Fe3O4, core-shell nanoparticle-catalyzed oxidative degradation of the antibiotic oxytetracycline in pre-treated landfill leachate. Chemosphere 191:639–650

    Article  Google Scholar 

  14. Yu X, Wan J, Shan Y, Chen K, Han X (2009) A facile approach to fabrication of bifunctional magnetic-optical Fe3O4@ZnS microspheres. Chem Mater 21:4892–4898

    Article  CAS  Google Scholar 

  15. Vazquez-Gonzalez M, Liao W, Cazelles R, Wang S, Yu X, Gutkin V, Willner I (2017) Mimicaking horseradish peroxidase functions using Cu2+ modified carbon nitride nanoparticles or Cu2+-modified carbon dots as heterogeneous catalysts. ACS Nano 11:3247–3253

    Article  CAS  Google Scholar 

  16. Wang S, Cazelles R, Liao W, Vazquez-Gonzalez M, Zoabi A, Reziq R, Willner I (2017) Mimicaking horseradish peroxidase and NADH peroxidase by heterogenous Cu-modified graphene oxide nanoparticles. Nano Lett 17:2043–2048

    Article  CAS  Google Scholar 

  17. Chen W, Vazquez-Gonzalez M, Kozell A, Cecconello A, Willner I (2018) Cu2+-modified metal-organic framework nanoparticles: a peroxidase-mimicking nanoenzyme. Small 14:1703149–1703156

    Article  Google Scholar 

  18. Wang Z, Chen Y, Dong W, Zhou J, Han B, Jiao J, Lan L, Miao P, Chen Q (2018) Copper(II)-poly-L-histidine functionalized multi walled carbon nanotubes as efficient mimetic enzyme for sensitive electrochemical detection of salvianic acid A. Biosens Bioelectron 121:257–264

    Article  CAS  Google Scholar 

  19. Xiong Y, Qin Y, Su L, Ye F (2017) Bioinspired synthesis of Cu2+-modified covalent triazine framework: a new highly efficient and promising peroxidase mimic. Chem Eur J 23:1–10

    Article  Google Scholar 

  20. Liang H, Lin F, Zhang Z, Liu B, Jiang S, Yuan Q, Liu J (2016) Multi-copper laccase mimicking nanozymes. ACS Appl Mater Interfaces 9:1352–1360

    Article  Google Scholar 

  21. Qin Y, Li Y, Liu X, Lu Z, Zheng L, Liu S, Cao Q, Ding Z (2017) Copper metal-organic polyhedral nanorods with high intrinsic peroxidase activity at physiological pH for bio-sensing. J Mater Chem B 5:9365–9370

    Article  CAS  Google Scholar 

  22. Menon S, Chandran S, Koyappayil A, Berchmans S (2018) Copper-based metal-organic frameworks as peroxidase mimics leading to sensitive H2O2 and glucose detection. Chem Select 3:8319–8324

    CAS  Google Scholar 

  23. Luo C, Wang X, Wang J, Pan K (2016) One-pot preparation of polyimide/Fe3O4, magnetic nanofibers with solvent resistant properties. Compos Sci Technol 133:97–103

    Article  CAS  Google Scholar 

  24. Wang Y, Wang X, Ding Y, Zhou Z, Hao C, Zhou Z (2018) Novel sodium lignosulphonate assisted synthesis of well dispersed Fe3O4, microspheres for efficient adsorption of copper(II). Powder Technol 325:597–605

    Article  CAS  Google Scholar 

  25. Guo J, Wang Y, Zhao M (2018) 3D flower-like ferrous(II) phosphate nanostructures as peroxidase mimetics for sensitive colorimetric detection of hydrogen peroxide and glucose at nanomolar level. Talanta 182:230–240

    Article  CAS  Google Scholar 

  26. Lin Y, Chang Y, Chen G, Chang Y (2009) Effects of Ag-doped NiTiO3 on photoreduction of methylene blue under UV and visible light irradiation. J Alloys Compd 479:785–790

    Article  CAS  Google Scholar 

  27. Li Y, Li J, Fu Y, Xie Q, Li Y (2019) Magnetic-core@dual-functional-shell nanocomposites with peroxidase mimicking properties for use in colorimetric and electrochemical sensing of hydrogen peroxide. Microchim Acta 186(1):20–28

    Article  Google Scholar 

  28. Sun Y, Wang R, Liu X, Shan G, Chen Y, Tong T, Liu Y (2018) Laser-induced formation of Au/Pt nanorods with peroxidase mimicking and SERS enhancement properties for application to the colorimetric determination of H2O2. Microchim Acta 185(9):445–454

    Article  Google Scholar 

  29. Wang N, Duan J, Shi W, Zhai X, Guan F, Yang L, Hou B (2018) A 3-dimensional C/CeO2 hollow nanostructure framework as a peroxidase mimetic, and its application to the colorimetric determination of hydrogen peroxide. Microchim Acta 185(9):417–425

    Article  Google Scholar 

  30. Dong Y, Zhang H, Rahman Z, Su L, Chen X, Hu J, Chen X (2012) Graphene oxide-Fe3O4 magnetic nanocomposites with peroxidase-like activity for colorimetric detection of glucose. Nanoscale 4:3969–3976

    Article  CAS  Google Scholar 

  31. Choleva TG, Gatselou VA, Tsogas GZ, Giokas DL (2018) Intrinsic peroxidase-like activity of rhodium nanoparticles, and their application to the colorimetric determination of hydrogen peroxide and glucose. Microchim Acta 185(1):22

    Article  Google Scholar 

  32. Gao L, Zhuang J, Nie L, Zhang J, Zhang Y, Gu N, Wang T, Feng J, Yang D, Perrett S (2007) Intrinsic peroxidase-like activity of ferromagnetic nanoparticles. Nat Nanotechnol 2:577–583

    Article  CAS  Google Scholar 

  33. Hu L, Yuan Y, Zhang J, Zhao J, Majeed S, Xu G (2013) Copper nanoclusters as peroxidase mimetics and their applications to H2O2 and glucose detection. Anal Chim Acta 762:83–86

    Article  CAS  Google Scholar 

  34. Ding Y, Yang B, Liu H, Liu Z, Zhang X, Zheng X, Liu Q (2018) FePt-Au ternary metallic nanoparticles with the enhanced peroxidase-like activity for ultrafast colorimetric detection of H2O2. Sensors Actuators B Chem 259:775–783

    Article  CAS  Google Scholar 

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Acknowledgements

This study was financed by the Science and Technology Program of Zhejiang Province of China (LGF18H200005), NSFC (21405029, and 61731008), the Medical and Health Technology Development Program of Zhejiang province (2017KY533), and the Young and Middle-aged Academic Leaders of Zhejiang Province.

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Authors and Affiliations

  1. Hangzhou Dianzi University, Hangzhou, 310018, China

    Hongying Liu, Langlang Zhu, Huan Ma, Jiajun Wen, Hanxiao Xu, Yubing Qiu, Linan Zhang & Lihua Li

  2. Deparment of Clinical Laboratory, Hangzhou Cancer Hospital, Hangzhou, 310002, China

    Chunchuan Gu

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  1. Hongying Liu
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  2. Langlang Zhu
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  3. Huan Ma
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  4. Jiajun Wen
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  6. Yubing Qiu
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  7. Linan Zhang
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  8. Lihua Li
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  9. Chunchuan Gu
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Correspondence to Linan Zhang, Lihua Li or Chunchuan Gu.

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Liu, H., Zhu, L., Ma, H. et al. Copper(II)-coated Fe3O4 nanoparticles as an efficient enzyme mimic for colorimetric detection of hydrogen peroxide. Microchim Acta 186, 518 (2019). https://doi.org/10.1007/s00604-019-3599-y

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