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Ectomycorrhizal fungal communities of pedunculate and sessile oak seedlings from bare-root forest nurseries

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Abstract

In this study, we present the detailed molecular investigation of the ectomycorrhizal (ECM) community of Quercus petraea and Quercus robur seedlings grown in bare-root forest nurseries. In all tested oak samples, mycorrhizal colonization was nearly 100%. Morphological observation and molecular investigations (sequencing of fungal ITS rDNA) revealed a total of 23 mycorrhizal taxa. The most frequent and abundant fungal taxa were Hebeloma sacchariolens, Tuber sp., and Peziza sp.; from the detected fungal taxa, 20 were noted for Q. petraea and 23 for Q. robur. Depending on the nursery, the species richness of identified ECM fungal taxa for both oak species ranged from six to 11 taxa. The mean species richness for all nurseries was 5.36 and 5.82 taxa per Q. petraea and Q. robur sample, respectively. According to the analysis of similarity, ECM fungal communities were similar for Q. petraea and Q. robur (R = 0.019; p = 0.151). On the other hand, detected fungal communities were significantly different between nurseries (R = 0.927; p < 0.0001). Using the Spearman rank correlation, it was determined that the ectomycorrhizal diversity (in terms of richness, the Shannon diversity, evenness, and Simpson dominance indices) is significantly related to the soil parameters of each nursery. We conclude that individual nursery may be considered as separate ecological niches that strongly discriminate diversity of ECM fungi.

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Acknowledgments

This study was supported by Institute of Dendrology Polish Academy of Sciences. We thank the Board of State Forest Districts in Gdańsk, Olsztyn, and Poznań and forest nursery staffs for the supply of experimental material and their help with fieldwork and in assembling background information. Prof. Jacek Oleksyn and two anonymous reviewers contributed valuable comments on final version of this manuscript.

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  1. Institute of Dendrology Polish Academy of Sciences, Kornik, Poland

    Tomasz Leski, Marcin Pietras & Maria Rudawska

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  1. Tomasz Leski
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  2. Marcin Pietras
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  3. Maria Rudawska
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Correspondence to Maria Rudawska.

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Leski, T., Pietras, M. & Rudawska, M. Ectomycorrhizal fungal communities of pedunculate and sessile oak seedlings from bare-root forest nurseries. Mycorrhiza 20, 179–190 (2010). https://doi.org/10.1007/s00572-009-0278-6

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