Abstract
Post-fire Pezizales fruit commonly in many forest types after fire. The objectives of this study were to determine which Pezizales appeared as sporocarps after a prescribed fire in the Blue Mountains of eastern Oregon, and whether species of Pezizales formed mycorrhizas on ponderosa pine, whether or not they were detected from sporocarps. Forty-two sporocarp collections in five genera (Anthracobia, Morchella, Peziza, Scutellinia, Tricharina) of post-fire Pezizales produced ten restriction fragment length polymorphism (RFLP) types. We found no root tips colonized by species of post-fire Pezizales fruiting at our site. However, 15% (6/39) of the RFLP types obtained from mycorrhizal roots within 32 soil cores were ascomycetes. Phylogenetic analyses of the 18S nuclear ribosomal DNA gene indicated that four of the six RFLP types clustered with two genera of the Pezizales, Wilcoxina and Geopora. Subsequent analyses indicated that two of these mycobionts were probably Wilcoxina rehmii, one Geopora cooperi, and one Geopora sp. The identities of two types were not successfully determined with PCR-based methods. Results contribute knowledge about the above- and below-ground ascomycete community in a ponderosa pine forest after a low intensity fire.
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Acknowledgements
We thank Keith Egger for his advice on E-strain fungi; Yana Valachovic and Bob Peck for field assistance; Art Fredeen, Annette Kretzer, and Hugues Massicotte for helpful comments on the paper. We are grateful to Chris Niwa and Walt Thies for coordinating research treatments, and to the Emigrant Creek Ranger District (previously the Burns Ranger District), especially Buddy Crisafi, Fire Manager, for help and timely burning of the stands. This study was funded by the USDA Forest Service, Pacific Northwest Research Station. Collections made by N.S. Weber were funded by National Science Foundation grants DEB 9007186 and BSR 9400545.
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Fujimura, K.E., Smith, J.E., Horton, T.R. et al. Pezizalean mycorrhizas and sporocarps in ponderosa pine (Pinus ponderosa) after prescribed fires in eastern Oregon, USA. Mycorrhiza 15, 79–86 (2005). https://doi.org/10.1007/s00572-004-0303-8
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DOI: https://doi.org/10.1007/s00572-004-0303-8