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Media formulation influences in vitro ectomycorrhizal synthesis on the European aspen Populus tremula L.

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Abstract

The effect of various media formulations on in vitro ectomycorrhizal synthesis of identified fungal strains with European aspen (Populus tremula L.) was tested in Petri dishes. Pre-grown seedlings were transferred to various nutrient media and inoculated with Paxillus involutus isolates using modified sandwich techniques. Mycorrhiza formation was evaluated macroscopically and further confirmed by microscopic examination of semi-thin sections for anatomical features of the mantle and the Hartig net. Standard media formulations did not support successful ectomycorrhiza formation because of either very poor plant survival (below 20%) or impaired fungal growth. The inclusion of micronutrients and vitamins in a Melin Norkrans (MMN)-based medium increased plant survival rate to above 60% and supported successful mycorrhizal synthesis. P. involutus isolates formed mycorrhizas with a characteristic Hartig net restricted to the epidermis. Mantle density and thickness varied depending on the isolate. In a follow-up experiment, the adapted medium supported successful ectomycorrhiza formation by various Laccaria and Hebeloma isolates. Our results show that an exogenous supply of vitamins and micronutrients in the medium was a prerequisite for successful mycorrhization of P. tremula in vitro in Petri dishes.

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Acknowledgments

We thank Ivano Brunner (WSL, Birmensdorf, Switzerland) for kindly providing the Swiss Paxillus involutus isolates WSL 37.7 and WSL 37.10 and Waltraud Klepal (Service Unit Cell Imaging and Ultrastructure Research; University of Vienna) for her most helpful support with sample preparation. Moreover, we thank Johann Glauninger (Institute of Plant Protection; BOKU) for kindly providing access to microscopic infrastructure and Siegrid Steinkellner (Institute of Plant Protection; BOKU) for her useful comments on the manuscript. The present study is part of the Project P170120-B04, which is financed by the Austrian Science Fund FWF.

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Authors and Affiliations

  1. Institute of Soil Science, University of Natural Resources and Applied Life Sciences, Peter Jordan-Straße 82, 1190, Vienna, Austria

    Ingrid Langer, Walter W. Wenzel & Peter Schweiger

  2. Institute of Microbiology, Innsbruck University, Technikerstraße 25, 6020, Innsbruck, Austria

    Doris Krpata & Ursula Peintner

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  1. Ingrid Langer
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  2. Doris Krpata
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  3. Ursula Peintner
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  4. Walter W. Wenzel
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  5. Peter Schweiger
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Correspondence to Ingrid Langer.

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Langer, I., Krpata, D., Peintner, U. et al. Media formulation influences in vitro ectomycorrhizal synthesis on the European aspen Populus tremula L.. Mycorrhiza 18, 297–307 (2008). https://doi.org/10.1007/s00572-008-0182-5

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