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In-vitro regeneration of olive tree by somatic embryogenesis

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Abstract

We induced somatic embryogenesis from the cotyledon segments ofOlea europaea (L) cvs. ‘Chetoui’, ‘Chemleli’, and ‘Arbequina’. Calli were established from all three cultvars on OMc media supplemented with IBA and 2i-R The greatest success was obtained with media that contained zero or low concentrations of growth regulators. High levels of hormones (i.e.,>0.5 mgL-1 IBA and 2i-P) inhibited embryogenesis. Embryos at different maturation stages were observed with continuously proliferating secondary embryogenesis. Abnormally shaped embryos and teratoma were also noted. Four weeks was the optimal incubation period for inducing embryogenesis on the auxin-containing medium. In addition, 30 to 40 gL-1 sucrose was more effective than glucose in stimulating the growth and maturation of somatic embryos. Embryogeic efficiency was also higher when multivariate combinations of nitrogen sources (inorganic and organic nitrogen forms) were used. The plantlets that were derived from our germinating somatic embryos were similar to those obtained from axillary buds.

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Abbreviations

Arb:

cultivar ‘Arbequina’

Ct:

cultivar ‘Chetoui’

Cm:

cultivar ‘Chemleli’

IBA:

lndole-3-butyric acid

2i-P:

isopentenyl adenine

OM:

Olive medium (Rugini, 1984)

OMc:

Olive modified medium (Canas and Benbadis, 1988)

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Authors and Affiliations

  1. Laboratory of Plant Biology and Biotechnology, Faculty of Sciences of Tunis Campus Universitaire, 1060, Tunis, Tunisia

    El Bahri Trabelsi & Sadok Bouzid

  2. lnstitut de lolivier de Tunisie, Rue Hedi Karray, 2049, Ariana, Tunisia

    El Bahri Trabelsi & Monji Bouzid

  3. National Institute of Agronomic Research of Tunisia, Rue Hedi Karray, 2049, Ariana, Tunisia

    Nedra Elloumi, Zina Belfeleh, Abdallah Benabdallah & Rachida Ghezel

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  1. El Bahri Trabelsi
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  2. Sadok Bouzid
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  3. Monji Bouzid
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  4. Nedra Elloumi
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  7. Rachida Ghezel
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Correspondence to El Bahri Trabelsi.

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Trabelsi, E.B., Bouzid, S., Bouzid, M. et al. In-vitro regeneration of olive tree by somatic embryogenesis. J. Plant Biol. 46, 173–180 (2003). https://doi.org/10.1007/BF03030446

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  • DOI: https://doi.org/10.1007/BF03030446

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