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Biomass, lipid content, and fatty acid composition of freshwater Chlamydomonas mexicana and Scenedesmus obliquus grown under salt stress

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Abstract

Two freshwater microalgae including Chlamydomonas mexicana and Scenedesmus obliquus were grown on Bold Basal Medium (BBM) with different levels of salinity up to 100 mM NaCl. The dry biomass and lipid content of microalgae were improved as the concentration of NaCl increased from 0 to 25 mM. Highest dry weight (0.8 and 0.65 g/L) and lipid content (37 and 34 %) of C. mexicana and S. obliquus, respectively, were obtained in BBM amended with 25 mM NaCl. The fatty acid composition of the investigated species was also improved by the increased NaCl concentration. At 50 mM, NaCl palmitic acid (35 %) and linoleic acid (41 %) were the dominant fatty acids in C. mexicana, while oleic acid (41 %) and α-linolenic acid (20 %) were the major fractions found in S. obliquus.

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Acknowledgments

This work was supported by the Korea Institute of Energy Research, the Senior Researchers program (The National Research Foundation of Korea, 2010-0026904), the Eco-Innovation project (The Global-Top project) funded by Korea Ministry of Environment, and the Brain Korea-21 (BK-21) and Brain Pool (KFSTS, Grant number: 11-150-152-1600-1658) programs administrated by the Ministry of Education, Science and Technology (MEST).

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Authors and Affiliations

  1. Department of Environmental Engineering, Yonsei University, Wonju, Gangwon-do, 220-710, South Korea

    El-Sayed Salama, Hyun-Chul Kim, Reda A. I. Abou-Shanab, Min-Kyu Ji, Seong-Heon Kim & Byong-Hun Jeon

  2. Department of Environmental Biotechnology, City of Scientific Research and Technology Applications, New Borg El Arab City, Alexandria, 21934, Egypt

    Reda A. I. Abou-Shanab

  3. Bioenergy Center, Korea Institute of Energy Research, Daejeon, 305-343, South Korea

    You-Kwan Oh

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  1. El-Sayed Salama
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  2. Hyun-Chul Kim
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Correspondence to Byong-Hun Jeon.

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Salama, ES., Kim, HC., Abou-Shanab, R.A.I. et al. Biomass, lipid content, and fatty acid composition of freshwater Chlamydomonas mexicana and Scenedesmus obliquus grown under salt stress. Bioprocess Biosyst Eng 36, 827–833 (2013). https://doi.org/10.1007/s00449-013-0919-1

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