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Improvement of laccase production and its properties by low-energy ion implantation

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Abstract

Low-energy ion implantation was employed to breed laccase producing strain Paecilomyces sp. WSH-L07 and a mutant S152 that exhibited an activity of more than three times over the wild strain was obtained. The optimum substrate of both the wild and mutant laccases was 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate), and followed by guaiacol with optimal pH at 3.4 and 5.0, respectively, while the mutant laccase exhibited a broader active pH range. The mutant laccase had a higher optimal catalytic temperature (60–65 °C) than the wild one (55 °C), and the wild laccase deactivated rapidly when temperature increased above 55 °C. Furthermore, the mutant laccase was more stable under neutral and alkaline conditions. A thermostability experiment revealed that the mutant laccase was superior to the wild laccase. Both laccases were stable in the presence of metal ions, mildly inhibited by SDS (0.5 mM), EDTA (1 mM) and 1,4-dithiothreitol (0.5 mM), and almost completely inhibited by 0.1 mM NaN3.

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Acknowledgments

We thank Mr. Yuan Hang in the key laboratory of Ion Beam Bioengineering of Chinese Academy of Science and Dr. Huang He in Nanjing University of Technology for their kindly helps in performing the ion implantation. This work was supported by the National Outstanding Youth Foundation of China (No. 20625619), the Major State Basic Research Development Program of China (973 Program) (No. 2007CB714306), the Program for Changjiang Scholars and Innovative Research Team in University (No. IRT0532), New Century Excellent Talents in University (NCET-07-0378).

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Authors and Affiliations

  1. Key Laboratory of Industrial Biotechnology, School of Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, Jiangsu, China

    Zhiyu Liu, Dongxu Zhang, Zhaozhe Hua, Jianghua Li, Guocheng Du & Jian Chen

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  1. Zhiyu Liu
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  2. Dongxu Zhang
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  3. Zhaozhe Hua
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  4. Jianghua Li
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  5. Guocheng Du
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  6. Jian Chen
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Correspondence to Guocheng Du or Jian Chen.

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Liu, Z., Zhang, D., Hua, Z. et al. Improvement of laccase production and its properties by low-energy ion implantation. Bioprocess Biosyst Eng 33, 639–646 (2010). https://doi.org/10.1007/s00449-009-0389-7

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  • DOI: https://doi.org/10.1007/s00449-009-0389-7

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