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Mutation breeding of chitosanase-producing strain Bacillus sp. S65 by low-energy ion implantation

  • Original Paper
  • Published:
Journal of Industrial Microbiology and Biotechnology

Abstract

In order to obtain an industrial strain with higher chitosanase yield, the wild strain Bacillus sp. S65 cells were mutated by a novel mutagen, nitrogen ion beam, with energy of 15 keV and dose ranging from 2.6 × 1014 to 5.2 × 1015 ions/cm2. One mutant, s65F5 with high yield of chitosanase was isolated. Results showed that the production of chitosanase of s65F5 was dramatically increased from 4.1 U/ml in s65 to 25 U/ml by ion beam implantation, while the fermentation time was shortened from 72 to 56 h, both of which greatly increased efficiency and reduced the cost of industrial production. Besides, the mutagenic effects of low-energy ion beam on survival rate showed characteristic down–up–down pattern, which was different from the traditional mutagens such as UV and γ-ray and the possible mutation mechanism was discussed.

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Acknowledgements

The authors thank Mr Zhang Shuqing and Yuan Hang in Key Laboratory of Ion Beam Bioengineering of Chinese Academy of Science for their help in performing the ion implantation. Financial support for this study from the Chinese Academy of Sciences is highly appreciated (Project KSCZ2-SW-324).

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Authors and Affiliations

  1. Ion Beam Bioengineering Key Lab, Institute of Plasma Physics, Chinese Academy of Sciences, 230031, Hefei 1126#, People’s Republic of China

    Caixin Su, Wei Zhou, Yonghong Fan, Li Wang, Shiguang Zhao & Zengliang Yu

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  1. Caixin Su
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  2. Wei Zhou
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  3. Yonghong Fan
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  4. Li Wang
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  5. Shiguang Zhao
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Correspondence to Zengliang Yu.

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Su, C., Zhou, W., Fan, Y. et al. Mutation breeding of chitosanase-producing strain Bacillus sp. S65 by low-energy ion implantation. J Ind Microbiol Biotechnol 33, 1037–1042 (2006). https://doi.org/10.1007/s10295-006-0155-7

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  • DOI: https://doi.org/10.1007/s10295-006-0155-7

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