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OPEN GLUME1: a key enzyme reducing the precursor of JA, participates in carbohydrate transport of lodicules during anthesis in rice

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Abstract

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OG1 is involved in JA-regulated anthesis by modulating carbohydrate transport of lodicules in rice.

Abstract

Flowering plants have evolved a sophisticated regulatory network to coordinate anthesis and maximize reproductive success. In addition to various environmental conditions, the plant hormone jasmonic acid and its derivatives (JAs) are involved in anthesis. However, the underlying mechanism remains largely unexplored. Here, we report a JA-defective mutant in rice (Oryza sativa), namely open glume 1, which has dysfunctional lodicules that lead to open glumes following anthesis. Map-based cloning and subsequent complementation tests confirmed that OG1 encodes a peroxisome-localized 12-oxo-phytodienoic acid reductase—a key enzyme that reduces the precursor of JA. Loss-of-function of OG1 resulted in almost no JA accumulation. Exogenous JA treatment completely rescued the defects caused by the og1 mutation. Further studies revealed that intracellular metabolism was disrupted in the lodicules of og1 mutant. At the mature plant stage, most seeds of the mutant were malformed with significantly reduced starch content. We speculate that JA or JA signaling mediates the carbohydrate transport of lodicules during anthesis, and signal the onset of cell degradation in lodicules after anthesis. We conclude that the OPEN GLUME 1 gene that produces a key enzyme involved in reducing the precursor of JA in JA biosynthesis and is involved in carbohydrate transport underlying normal lodicule function during anthesis in rice.

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Abbreviations

JA:

Jasmonic acid

OPDA:

12-oxo-phytodienoic acid

OPC 8:0:

3-oxo-2-(2′-pentenyl)-cyclopentane-1-octanoic acid

OPR:

12-oxo-phytodienoic acid reductase

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Acknowledgements

We thank Prof. Xiangning Jiang (Beijing Forestry University) for technical guidance on HPLC-MS, and the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences for measurement of endogenous JA content. We also acknowledge support from the Key Laboratory of Biology, Genetics and Breeding of Japonica Rice in Mid-lower Yangtze River, Ministry of Agriculture, P.R. China, and Jiangsu Collaborative Innovation Center for Modern Crop Production, This research was supported by grants from The National Key Research and Development Program of China (Grant: 2016YFD0101801), 863 National High-tech R&D Program of China (2014AA10A603-15), Jiangsu Science and Technology Development Program (BE2014394), and Jiangsu Agricultural Science and Technology Innovation Fund Project (CX(16)1029).

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    1. State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

      Xiaohui Li, Yihua Wang, Erchao Duan, Kunneng Zhou, Qiuyun Lin, Di Wang, Yunlong Wang, Wuhua Long, Zhigang Zhao, Ling Jiang, Chunming Wang & Jianmin Wan

    2. National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, People’s Republic of China

      Zhijun Cheng, Cailin Lei, Xin Zhang, Xiuping Guo, Jiulin Wang, Chuanyin Wu & Jianmin Wan

    3. College of Life Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, People’s Republic of China

      Qi Qi

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    Communicated by Kang Chong.

    Xiaohui Li and Yihua Wang contributed equally to this work.

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    Li, X., Wang, Y., Duan, E. et al. OPEN GLUME1: a key enzyme reducing the precursor of JA, participates in carbohydrate transport of lodicules during anthesis in rice. Plant Cell Rep 37, 329–346 (2018). https://doi.org/10.1007/s00299-017-2232-y

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