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Identification of the OsOPR7 gene encoding 12-oxophytodienoate reductase involved in the biosynthesis of jasmonic acid in rice

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Abstract

Enzyme 12-oxophytodienoate (OPDA) reductase (EC1.3.1.42), which is involved in the biosynthesis of jasmonic acid (JA), catalyses the reduction of 10, 11-double bonds of OPDA to yield 3-oxo-2-(2′-pentenyl)-cyclopentane-1-octanoic acid (OPC-8:0). The rice OsOPR1 gene encodes OPDA reductase (OPR) converting (−)-cis-OPDA preferentially, rather than (+)-cis-OPDA, a natural precursor of JA. Here, we provide evidence that an OPR family gene in rice chromosome 8, designated OsOPR7, encodes the enzyme involved in the JA biosynthesis. Recombinant OsOPR7-His protein efficiently catalysed the reduction of both enantiomers of cis-OPDA, similar to the OPR3 protein in Arabidopsis thaliana (L.) Heynh. The expression of OsOPR7 mRNA was induced and reached maximum levels within 0.5 h of mechanical wounding and drought stress, and the endogenous JA level started to increase in accordance with the increase in OsOPR7 expression. The GFP-OsOPR7 fusion protein was detected exclusively in peroxisomes in onion epidermal cells. Furthermore, complementation analysis using an Arabidopsis opr3 mutant indicated that the OsOPR7 gene, but not OsOPR1, was able to complement the phenotypes of male sterility in the mutant caused by JA deficiency, and that JA production in the opr3 mutant was also restored by the expression of the OsOPR7 gene. We conclude that the OsOPR7 gene encodes the enzyme catalysing the reduction of natural (+)-cis-OPDA for the JA biosynthesis in rice.

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Abbreviations

GC–MS:

Gas chromatography–mass spectrometry

GFP:

Green fluorescent protein

JA:

Jasmonic acid

LC–MS/MS:

Liquid chromatography–mass spectrometry/mass spectrometry

OPC-8:0:

3-Oxo-2-(2′-pentenyl)-cyclopentane-1-octanoic acid

OPDA:

12-Oxophytodienoic acid

OPR:

12-Oxophytodienoic acid reductase

RFP:

Red fluorescent protein

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Acknowledgments

We are most grateful to Dr. Tsuyoshi Nakagawa (Shimane University) for providing the pGWB series vectors, and Dr. Yusuke Jikumaru (Plant Science Center, RIKEN) for technical advice for JA measurement using LC-MS/MS. We also thank the ABRC for providing seeds of the opr3 mutant. This work was supported in part by a Grant-in-Aid for Scientific Research (No. 15380080) to H.Y. from the Japanese Society for the Promotion of Science, and by the Program for the Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN).

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Authors and Affiliations

  1. Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Tomoyuki Tani, Hiroyuki Sobajima, Kazunori Okada, Tetsuya Chujo, Hideaki Nojiri & Hisakazu Yamane

  2. Laboratory of Plant Molecular Genetics, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Shin-ichi Arimura & Nobuhiro Tsutsumi

  3. Department of Cell Biology, National Institute for Basic Biology, Okazaki, 444-8585, Japan

    Mikio Nishimura

  4. The Institute of Physical and Chemical Research (RIKEN), 2-1 Hirosawa, Wako-shi, Saitama, 351-0198, Japan

    Hideharu Seto

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  1. Tomoyuki Tani
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  2. Hiroyuki Sobajima
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  3. Kazunori Okada
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  7. Mikio Nishimura
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  8. Hideharu Seto
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  9. Hideaki Nojiri
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  10. Hisakazu Yamane
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Correspondence to Kazunori Okada.

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Tomoyuki Tani and Hiroyuki Sobajima have equally contributed to this work.

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Tani, T., Sobajima, H., Okada, K. et al. Identification of the OsOPR7 gene encoding 12-oxophytodienoate reductase involved in the biosynthesis of jasmonic acid in rice. Planta 227, 517–526 (2008). https://doi.org/10.1007/s00425-007-0635-7

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