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Transient silencing of the grapevine gene VvPGIP1 by agroinfiltration with a construct for RNA interference

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Abstract

Grapevine is an economically important crop, and the recent completion of its genome makes it possible to study the function of specific genes through reverse genetics. However, the analysis of gene function by RNA interference (RNAi) in grapevine is difficult, because the generation of stable transgenic plants has low efficiency and is time consuming. Recently, transient expression of genes in grapevine leaves has been obtained by Agrobacterium tumefaciens infiltration (agroinfiltration). We therefore tested the possibility to silence grapevine genes by agroinfiltration of RNAi constructs. A construct to express a double strand RNA (dsRNA) corresponding to the defense-related gene VvPGIP1, encoding a polygalacturonase-inhibiting protein (PGIP), was obtained and transiently expressed by agroinfiltration in leaves of grapevine plants grown in vitro. Expression of VvPGIP1 and accumulation of PGIP activity were strongly induced by infiltration with control bacteria, but not with bacteria carrying the dsRNA construct, indicating that the gene was efficiently silenced. In contrast, expression of another defense-related gene, VST1, encoding a stilbene synthase, was unaffected by the dsRNA construct. We have therefore demonstrated the possibility of transient down-regulation of grapevine genes by agroinfiltration of constructs for the expression of dsRNA. This system can be employed to evaluate the effectiveness of constructs that can be subsequently used to generate stable RNAi transgenic plants.

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References

  • Bezier A, Lambert B, Baillieul F (2002) Study of defense-related gene expression in grapevine leaves and berries infected with Botrytis cinerea. Eur J Pl Pathol 108:111–120

    Article  CAS  Google Scholar 

  • Bhaskar PB, Venkateshwaran M, Wu L, Ane JM, Jiang J (2009) Agrobacterium-mediated transient gene expression and silencing: a rapid tool for functional gene assay in potato. PLoS ONE 4:e5812. doi:10.1371/journal.pone.0005812

    Article  PubMed  Google Scholar 

  • Cervone F, De Lorenzo G, Degrà L, Salvi G, Bergami M (1987) Purification and characterization of a polygalacturonase-inhibiting protein from Phaseolus vulgaris L. Plant Physiol 85:631–637

    Article  PubMed  CAS  Google Scholar 

  • Chapman EJ, Carrington JC (2007) Specialization and evolution of endogenous small RNA pathways. Nat Rev Genet 8:884–896

    Article  PubMed  CAS  Google Scholar 

  • Chuang CF, Meyerowitz EM (2000) Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana. Proc Natl Acad Sci USA 97:4985–4990

    Article  PubMed  CAS  Google Scholar 

  • Coutos-Thevenot P, Poinssot B, Bonomelli A, Yean H, Breda C, Buffard D, Esnault R, Hain R, Boulay M (2001) In vitro tolerance to Botrytis cinerea of grapevine 41B rootstock in transgenic plants expressing the stilbene synthase Vst1 gene under the control of a pathogen-inducible PR 10 promoter. J Exp Bot 52:901–910

    Article  PubMed  CAS  Google Scholar 

  • Davies C, Boss PK (2000) The use of molecular biology techniques to study and manipulate the grapevine: why and how? Aust J Grape Wine Res 6:159–167

    Article  CAS  Google Scholar 

  • De Lorenzo G, Ferrari S (2002) Polygalacturonase-inhibiting proteins in defense against phytopathogenic fungi. Curr Opin Plant Biol 5:295–299

    Article  PubMed  Google Scholar 

  • Dutt M, Li Z, Dhekney S, Gray D (2007) Transgenic plants from shoot apical meristems of Vitis vinifera L. ‘Thompson Seedless’ via Agrobacterium-mediated transformation. Plant Cell Rep 26:2101–2110

    Article  PubMed  CAS  Google Scholar 

  • Elad Y, Williamson B, Tudzynski P, Delen N (2007) Botrytis spp. and diseases they cause in agricultural systems—an introduction. In: Elad Y, Williamson B, Tudzynski P, Delen N (eds) Botrytis: biology, pathology and control. Springer, Netherlands, pp 1–8

    Chapter  Google Scholar 

  • Ferrari S, Galletti R, Vairo D, Cervone F, De Lorenzo G (2006) Antisense expression of the Arabidopsis thaliana AtPGIP1 gene reduces polygalacturonase-inhibiting protein accumulation and enhances susceptibility to Botrytis cinerea. Mol Plant Microbe Interact 19:931–936

    Article  PubMed  CAS  Google Scholar 

  • Ferrari S, Sella L, Janni M, De Lorenzo G, Favaron F, D’Ovidio R (2011) Transgenic expression of polygalacturonase-inhibiting proteins in Arabidopsis and wheat increases resistance to the flower pathogen Fusarium graminearum. Plant Biol. doi:10.1111/j.1438-8677.2011.00449.x

  • Ferrari S, Vairo D, Ausubel FM, Cervone F, De Lorenzo G (2003) Tandemly duplicated Arabidopsis genes that encode polygalacturonase-inhibiting proteins are regulated coordinately by different signal transduction pathways in response to fungal infection. Plant Cell 15:93–106

    Article  PubMed  CAS  Google Scholar 

  • Figueiredo A, Fortes AM, Ferreira S, Sebastiana M, Choi YH, Sousa L, Acioli-Santos B, Pessoa F, Verpoorte R, Pais MS (2008) Transcriptional and metabolic profiling of grape (Vitis vinifera L.) leaves unravel possible innate resistance against pathogenic fungi. J Exp Bot 59:3371–3381

    Article  PubMed  CAS  Google Scholar 

  • Frizzi A, Huang S (2010) Tapping RNA silencing pathways for plant biotechnology. Plant Biotechnol J 8:655–677

    Article  PubMed  CAS  Google Scholar 

  • Ghildiyal M, Zamore PD (2009) Small silencing RNAs: an expanding universe. Nat Rev Genet 10:94–108

    Article  PubMed  CAS  Google Scholar 

  • Gleave AP (1992) A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genome. Plant Mol Biol 20:1203–1207

    Article  PubMed  CAS  Google Scholar 

  • Hain R, Bieseler B, Kindl H, Schröder G, Stöcker R (1990) Expression of a stilbene synthase gene in Nicotiana tabacum results in synthesis of the phytoalexin resveratrol. Plant Mol Biol 15:325–335

    Article  PubMed  CAS  Google Scholar 

  • Hellens RP, Edwards EA, Leyland NR, Bean S, Mullineaux PM (2000) pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Mol Biol 42:819–832

    Article  PubMed  CAS  Google Scholar 

  • Helm JM, Dadami E, Kalantidis K (2011) Local RNA silencing mediated by agroinfiltration. Methods Mol Biol 744:97–108

    Article  PubMed  CAS  Google Scholar 

  • Iocco P, Franks T, Thomas MR (2001) Genetic Transformation of Major Wine Grape Cultivars of Vitis vinifera L. Transgenic Res 10:105–112

    Article  PubMed  CAS  Google Scholar 

  • Jaillon O, Aury JM, Noel B, Policriti A, Clepet C, Casagrande A, Choisne N, Aubourg S, Vitulo N, Jubin C, Vezzi A, Legeai F, Hugueney P, Dasilva C, Horner D, Mica E, Jublot D, Poulain J, Bruyere C, Billault A, Segurens B, Gouyvenoux M, Ugarte E, Cattonaro F, Anthouard V, Vico V, Del Fabbro C, Alaux M, Di Gaspero G, Dumas V, Felice N, Paillard S, Juman I, Moroldo M, Scalabrin S, Canaguier A, Le CI, Malacrida G, Durand E, Pesole G, Laucou V, Chatelet P, Merdinoglu D, Delledonne M, Pezzotti M, Lecharny A, Scarpelli C, Artiguenave F, Pe ME, Valle G, Morgante M, Caboche M, Adam-Blondon AF, Weissenbach J, Quetier F, Wincker P (2007) The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla. Nature 449:463–467

    Google Scholar 

  • Janni M, Sella L, Favaron F, Blechl AE, De Lorenzo G, D’Ovidio R (2008) The expression of a bean polygalacturonase-inhibiting proteins in transgenic wheat confers increased resistance to the fungal pathogen Bipolaris sorokiniana. Mol Plant Microbe Interact 21:171–177

    Article  PubMed  CAS  Google Scholar 

  • Jarvis WR (1977) Botryotinia and Botrytis species: taxonomy, physiology, and pathogenicity: a guide to the literature. Canada Department of Agriculture, Ottawa

    Google Scholar 

  • Jeandet P, Douillet-Breuil AC, Bessis R, Debord S, Sbaghi M, Adrian M (2002) Phytoalexins from the Vitaceae: biosynthesis, phytoalexin gene expression in transgenic plants, antifungal activity, and metabolism. J Agric Food Chem 50:2731–2741

    Article  PubMed  CAS  Google Scholar 

  • Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901–3907

    PubMed  CAS  Google Scholar 

  • Joubert DA, Slaughter AR, Kemp G, Becker JV, Krooshof GH, Bergmann C, Benen J, Pretorius IS, Vivier MA (2006) The grapevine polygalacturonase-inhibiting protein (VvPGIP1) reduces Botrytis cinerea susceptibility in transgenic tobacco and differentially inhibits fungal polygalacturonases. Transgenic Res 15:687–702

    Article  PubMed  CAS  Google Scholar 

  • Katagiri F, Thilmony R, He SY (2002) The Arabidopsis thaliana–Pseudomonas syringae interaction. The Arabidopsis Book, pp 1–35

  • Kikkert JR, Thomas MR, Reisch BI (2001) Grapevine genetic engineering. In: Roubelakis-Angelakis KA (ed) Molecular biology and biotechnology of the Grapevine. Kluwer, Dordrecht, pp 393–410

    Google Scholar 

  • Langcake P, Pryce RJ (1977) A new class of phytoalexins from grapevines. Experientia 33:151–152

    Article  PubMed  CAS  Google Scholar 

  • Lee MW, Yang Y (2006) Transient expression assay by agroinfiltration of leaves. Methods Mol Biol 323:225–229

    PubMed  Google Scholar 

  • Li Z, Dhekney S, Dutt M, Gray D (2008) An improved protocol for Agrobacterium-mediated transformation of grapevine (Vitis vinifera L.). Plant Cell Tissue Org Culture 93:311–321

    Article  Google Scholar 

  • Lionetti V, Raiola A, Camardella L, Giovane A, Obel N, Pauly M, Favaron F, Cervone F, Bellincampi D (2007) Overexpression of pectin methylesterase inhibitors in Arabidopsis restricts fungal infection by Botrytis cinerea. Plant Physiol 143:1871–1880

    Article  PubMed  CAS  Google Scholar 

  • Liu J, Li H, Miao M, Tang X, Giovannoni J, Xiao F, Liu Y (2011) The tomato UV-damaged DNA-binding protein-1 (DDB1) is implicated in pathogenesis-related (PR) gene expression and resistance to Agrobacterium tumefaciens. Mol Plant Pathol (in press). doi:10.1111/j.1364-3703.2011.00735.x

  • Luria SE, Burrous JW (1957) Hybridization between Escherichia coli and Shigella. J Bacteriol 74:461–476

    PubMed  CAS  Google Scholar 

  • MacKenzie DJ, Mclean MA, Mukerji S, Green M (1997) Improved RNA extraction from woody plants for the detection of viral pathogens by reverse transcription-polymerase chain reaction. Plant Dis 81:222–226

    Article  CAS  Google Scholar 

  • Mezzetti B, Pandolfini T, Navacchi O, Landi L (2002) Genetic transformation of Vitis vinifera via organogenesis. BMC Biotechnol 2:18

    Article  PubMed  Google Scholar 

  • Milner Y, Avigad G (1967) A copper reagent for the determination of hexuronic acids and certain ketohexoses. Carbohydr Res 4:359–361

    Article  CAS  Google Scholar 

  • Moroldo M, Paillard S, Marconi R, Fabrice L, Canaguier A, Cruaud C, De B, V, Guichard C, Brunaud V, Le CI, Scalabrin S, Testolin R, Di Gaspero G, Morgante M, Adam-Blondon AF (2008) A physical map of the heterozygous grapevine ‘Cabernet Sauvignon’ allows mapping candidate genes for disease resistance. BMC Plant Biol 8:66

  • Murashige T, Skoog F (1962) Revised medium for rapid growth and bioassays with tobacco cultures. Physiol Plant 15:437–479

    Article  Google Scholar 

  • Muruganantham M, Moskovitz Y, Haviv S, Horesh T, Fenigstein A, Preez J, Stephan D, Burger JT, Mawassi M (2009) Grapevine virusA-mediated gene silencing in Nicotiana benthamiana and Vitis vinifera. J Virol Methods 155:167–174

    Article  PubMed  CAS  Google Scholar 

  • Peng FY, Reid KE, Liao N, Schlosser J, Lijavetzky D, Holt R, Martinez Zapater JM, Jones S, Marra M, Bohlmann J, Lund ST (2007) Generation of ESTs in Vitis vinifera wine grape (Cabernet Sauvignon) and table grape (Muscat Hamburg) and discovery of new candidate genes with potential roles in berry development. Gene 402:40–50

    Article  PubMed  CAS  Google Scholar 

  • Peretz Y, Mozes-Koch R, Akad F, Tanne E, Czosnek H, Sela I (2007) A universal expression/silencing vector in plants. Plant Physiol 145:1251–1263

    Article  PubMed  CAS  Google Scholar 

  • Perl A, Eshdat Y (1998) DNA transfer and gene expression in transgenic grapes. Genet Eng Rev 15:365–386

    CAS  Google Scholar 

  • Perl A, Lotan O, Abu-Abied M, Holland D (1996) Establishment of an Agrobacterium-mediated transformation system for grape (Vitis vinifera L.): the role of antioxidants during grape–Agrobacterium interactions. Nat Biotechnol 14:624–628

    Article  PubMed  CAS  Google Scholar 

  • Polesani M, Bortesi L, Ferrarini A, Zamboni A, Fasoli M, Zadra C, Lovato A, Pezzotti M, Delledonne M, Polverari A (2010) General and species-specific transcriptional responses to downy mildew infection in a susceptible (Vitis vinifera) and a resistant (V. riparia) grapevine species. BMC Genomics 11:117

    Article  PubMed  Google Scholar 

  • Powell AL, van Kan J, ten Have A, Visser J, Greve LC, Bennett AB, Labavitch JM (2000) Transgenic expression of pear PGIP in tomato limits fungal colonization. Mol Plant Microbe Interact 13:942–950

    Article  PubMed  CAS  Google Scholar 

  • Pruss GJ, Nester EW, Vance V (2008) Infiltration with Agrobacterium tumefaciens induces host defense and development-dependent responses in the infiltrated zone. Mol Plant Microbe Interact 21:1528–1538

    Article  PubMed  CAS  Google Scholar 

  • Rico A, Bennett MH, Forcat S, Huang WE, Preston GM (2010) Agroinfiltration reduces ABA levels and suppresses Pseudomonas syringae-elicited salicylic acid production in Nicotiana tabacum. PLoS ONE 5:e8977

    Article  PubMed  Google Scholar 

  • Salmaso M, Valle RD, Lucchin M (2008) Gene pool variation and phylogenetic relationships of an indigenous northeast Italian grapevine collection revealed by nuclear and chloroplast SSRs. Genome 51:838–855

    Article  PubMed  CAS  Google Scholar 

  • Santos-Rosa M, Poutaraud A, Merdinoglu D, Mestre P (2008) Development of a transient expression system in grapevine via agro-infiltration. Plant Cell Rep 27:1053–1063

    Article  PubMed  CAS  Google Scholar 

  • Scalabrin S, Troggio M, Moroldo M, Pindo M, Felice N, Coppola G, Prete G, Malacarne G, Marconi R, Faes G, Jurman I, Grando S, Jesse T, Segala C, Valle G, Policriti A, Fontana P, Morgante M, Velasco R (2010) Physical mapping in highly heterozygous genomes: a physical contig map of the Pinot Noir grapevine cultivar. BMC Genomics 11:204

    Article  PubMed  Google Scholar 

  • Sella L, Castiglioni C, Roberti S, D’Ovidio R, Favaron F (2004) An endo-polygalacturonase (PG) of Fusarium moniliforme escaping inhibition by plant polygalacturonase-inhibiting proteins (PGIPs) provides new insights into the PG–PGIP interaction. FEMS Microbiol Lett 240:117–124

    Article  PubMed  CAS  Google Scholar 

  • Small I (2007) RNAi for revealing and engineering plant gene functions. Curr Opin Biotechnol 18:148–153

    Article  PubMed  CAS  Google Scholar 

  • Sparkes IA, Runions J, Kearns A, Hawes C (2006) Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants. Nat Protoc 1:2019–2025

    Article  PubMed  CAS  Google Scholar 

  • Sparvoli F, Martin C, Scienza A, Gavazzi G, Tonelli C (1994) Cloning and molecular analysis of structural genes involved in flavonoid and stilbene biosynthesis in grape (Vitis vinifera L.). Plant Mol Biol 24:743–755

    Article  PubMed  CAS  Google Scholar 

  • Szécsi A (1990) Analysis of pectic enzyme zymograms of Fusarium species. I. Fusarium lateritium and related species. J Phytopathol 128:75–83

    Article  Google Scholar 

  • Tattersall EA, Grimplet J, DeLuc L, Wheatley MD, Vincent D, Osborne C, Ergul A, Lomen E, Blank RR, Schlauch KA, Cushman JC, Cramer GR (2007) Transcript abundance profiles reveal larger and more complex responses of grapevine to chilling compared to osmotic and salinity stress. Funct Integr Genomics 7:317–333

    Article  PubMed  CAS  Google Scholar 

  • Taylor RJ, Secor GA (1988) An improved diffusion assay for quantifying the polygalacturonase content of Erwinia culture filtrates. Phytopathology 78:1101–1103

    Article  CAS  Google Scholar 

  • ten Have A, Mulder W, Visser J, van Kan JA (1998) The endopolygalacturonase gene Bcpg1 is required for full virulence of Botrytis cinerea. Mol Plant Microbe Interact 11:1009–1016

    Article  PubMed  CAS  Google Scholar 

  • Velasco R, Zharkikh A, Troggio M, Cartwright DA, Cestaro A, Pruss D, Pindo M, Fitzgerald LM, Vezzulli S, Reid J, Malacarne G, Iliev D, Coppola G, Wardell B, Micheletti D, Macalma T, Facci M, Mitchell JT, Perazzolli M, Eldredge G, Gatto P, Oyzerski R, Moretto M, Gutin N, Stefanini M, Chen Y, Segala C, Davenport C, Dematte L, Mraz A, Battilana J, Stormo K, Costa F, Tao Q, Si-Ammour A, Harkins T, Lackey A, Perbost C, Taillon B, Stella A, Solovyev V, Fawcett JA, Sterck L, Vandepoele K, Grando SM, Toppo S, Moser C, Lanchbury J, Bogden R, Skolnick M, Sgaramella V, Bhatnagar SK, Fontana P, Gutin A, Van de PY, Salamini F, Viola R (2007) A high quality draft consensus sequence of the genome of a heterozygous grapevine variety. PLoS ONE 2:e1326

    Article  PubMed  Google Scholar 

  • Vidal JR, Kikkert JR, Wallace PG, Reisch BI (2003) High-efficiency biolistic co-transformation and regeneration of ‘Chardonnay’ (Vitis vinifera L.) containing npt-II and antimicrobial peptide genes. Plant Cell Rep 22:252–260

    Article  PubMed  CAS  Google Scholar 

  • Vivier MA, Pretorius IS (2000) Genetic improvement of grapevine: tailoring grape varieties for the third millennium—a review. South Afr J Enol Viticult 21:5–26

    CAS  Google Scholar 

  • Voinnet O (2009) Origin, biogenesis, and activity of plant microRNAs. Cell 136:669–687

    Article  PubMed  CAS  Google Scholar 

  • Weller SA, Elphinstone JG, Smith NC, Boonham N, Stead DE (2000) Detection of Ralstonia solanacearum strains with a quantitative, multiplex, real-time, fluorogenic PCR (TaqMan) assay. Appl Environ Microbiol 66:2853–2858

    Article  PubMed  CAS  Google Scholar 

  • Wesley SV, Helliwell CA, Smith NA, Wang MB, Rouse DT, Liu Q, Gooding PS, Singh SP, Abbott D, Stoutjesdijk PA, Robinson SP, Gleave AP, Green AG, Waterhouse PM (2001) Construct design for efficient, effective and high-throughput gene silencing in plants. Plant J 27:581–590

    Article  PubMed  CAS  Google Scholar 

  • Wiese W, Vornam B, Krause E, Kindl H (1994) Structural organization and differential expression of three stilbene synthase genes located on a 13 kb grapevine DNA fragment. Plant Mol Biol 26:667–677

    Article  PubMed  CAS  Google Scholar 

  • Wroblewski T, Tomczak A, Michelmore R (2005) Optimization of Agrobacterium-mediated transient assays of gene expression in lettuce, tomato and Arabidopsis. Plant Biotechnol J 3:259–273

    Article  PubMed  CAS  Google Scholar 

  • Wubben JP, Mulder W, ten Have A, van Kan JA, Visser J (1999) Cloning and partial characterization of endopolygalacturonase genes from Botrytis cinerea. Appl Environ Microbiol 65:1596–1602

    PubMed  CAS  Google Scholar 

  • Yang Y, Li R, Qi M (2000) In vivo analysis of plant promoters and transcription factors by agroinfiltration of tobacco leaves. Plant J 22:543–551

    Article  PubMed  CAS  Google Scholar 

  • Zottini M, Barizza E, Costa A, Formentin E, Ruberti C, Carimi F, Lo Schiavo F (2008) Agroinfiltration of grapevine leaves for fast transient assays of gene expression and for long-term production of stable transformed cells. Plant Cell Rep 27:845–853

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This study was supported by the Ministero per l’Istruzione, l’Università e la Ricerca Scientifica (PRIN2006 grant awarded to S.F.), the European Research Council (ERC Advanced Grant no. 233083) and the Ministero per le Politiche Agricole, Alimentari e Forestali (BIOMASSVAL grant). The authors are very grateful to L. Miotti, L. Filippin, G. Da Rold and I. Bazzo (CRA-VIT) for their technical assistance.

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  1. CRA-VIT Centro di ricerca per la viticoltura, Viale XXVIII Aprile 26, 31015, Conegliano, TV, Italy

    Nadia Bertazzon, Massimo Gardiman, Elisa Angelini & Michele Borgo

  2. Dipartimento Territorio e Sistemi Agro-forestali, Università degli Studi di Padova, Viale dell’Università 16, 35020, Legnaro, PD, Italy

    Alessandro Raiola & Carla Castiglioni

  3. Dipartimento di Biologia e Biotecnologie “Charles Darwin”, Sapienza Università di Roma, Piazzale Aldo Moro 5, 00185, Rome, Italy

    Simone Ferrari

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  1. Nadia Bertazzon
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  2. Alessandro Raiola
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Correspondence to Simone Ferrari.

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Communicated by L. Peña.

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Bertazzon, N., Raiola, A., Castiglioni, C. et al. Transient silencing of the grapevine gene VvPGIP1 by agroinfiltration with a construct for RNA interference. Plant Cell Rep 31, 133–143 (2012). https://doi.org/10.1007/s00299-011-1147-2

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