Abstract
Radish is a major root crop grown in the Far East and is especially important to some low-income countries where it is consumed on a daily basis. Developments in gene technology systems have helped to accelerate the production of useful germplasms, but progress has been slow, though achieved, via in planta methods and useful traits have been introduced. In the wake of the new Millennium, future goals in terms of improving transformation efficiency and selection of new traits for generating late-flowering radish are described. Furthermore, the techniques available for incorporating pharmaceutical proteins into radish to deliver edible proteins on-site are discussed. Finally, the concerns of releasing transgenic radish to the field in terms of pollen-mediated gene transfer are also reviewed. Such a report identifies key areas of research that is required to allow the crop satisfy the need of poor impoverished countries in the Far East.
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Babu RC, Zhang J, Blum A, Ho DT-H, Wu R, Nguyen HT (2004) HVA1, a LEA gene from barley confers dehydration tolerance in transgenic rice (Oryza sativa L.) via cell membrane protection. Plant Sci 166:855–862
Ballester A, Cervera M, Pena L (2008) Evaluation of selection strategies alternative to nptII in genetic transformation of citrus. Plant Cell Rep 27:1005–1015
Bechtold N, Ellis J, Pelletier G (1993) In plant Agrobacterium-mediated transformation gene transfer by infiltration of adult Arabidopsis thaliana plants. C R Acad Sci Paris Life Sci 316:1194–1199
Becker C (1962) Rettish und radies (Raphanus sativus). Handbuch der Pflanzenzuchtung 6:23–78
Bhatnagar M, Prasad K, Bhatnagar-Mathur P, Narasu ML, Waliyar F, Sharma KK (2010) An efficient method for the production of marker-free transgenic plants of peanut (Arachis hypogaea L.). Plant Cell Rep 29:495–502
Burnett L, Arnoldo M, Yarrow S, Huang B (1994) Enhancement of shoot regeneration from cotyledon explants of Brassica rapa ssp. oleifera through pretreatment with auxin and cytokinin and use of ethylene inhibitors. Plant Cell Tissue Organ Cult 37:253–256
Chang SS, Park SK, Kim BC, Kang BJ, Kim DU, Nam HG (1994) Stable genetic transformation of Arabidopsis thaliana by Agrobacterium inoculation in planta. Plant J 5:551–558
Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16:735–743
Crisp P (1995) Radish. In: Smartt J, Simmonds NW (eds) Evolution of crop plants. Longman Scientific & Technical, London, pp 86–89
Curtis IS (2004) Genetic transformation of radish (Raphanus sativus L.) by floral-dipping. In: Curtis IS (ed) Transgenic crops of the world-essential protocols. Kluwer Academic Publishers, Dordrecht, pp 271–280
Curtis IS (2008) Radish. In: Kole C and Hall TC (eds) Compendium of transgenic crop plants: transgenic vegetable crops, vol 6, chap 5. Wiley, Chichester, pp 117–134
Curtis IS, Nam HG (2001) Transgenic radish (Raphanus sativus L. var. longipinnatus Bailey) by floral-dip method–plant development and surfactant are important in optimizing transformation efficiency. Trans Res 10:363–371
Curtis IS, Nam HG, Yun JY, Seo K-H (2002) Expression of an antisense GIGANTEA (GI) gene fragment in transgenic radish causes delayed bolting and flowering. Trans Res 11:249–256
Curtis IS, Nam HG, Sakamoto K (2004) Optimized shoot regeneration system for the commercial Korean radish ‘Jin Ju Dae Pyong’. Plant Cell Tissue Organ Cult 77:81–87
Curtis IS, Hanada A, Yamaguchi S, Kamiya Y (2005) Modification of plant architecture through the expression of GA 2-oxidase under the control of an estrogen inducible promoter in Arabidopsis thaliana L. Planta 222:957–967
Dai XG, Shi XP, Ye YM, Fu Q, Bao MZ (2009) High frequency plant regeneration from cotyledon and hypocotyl explants of ornamental kale. Biol Plant 53:769–773
Dale EC, Ow DW (1991) Gene transfer with subsequent removal of the selection gene from the host genome. Proc Natl Acad Sci USA 88:10558–10562
Daniell H (2006) Production of biopharmaceuticals and vaccines in plants via the chloroplast genome. Biotechnol J 1:1071–1079
Darmency H (1994) The impact of hybrids between genetically modified crop plants and their related species: introgression and weediness. Mol Ecol 3:37–40
De Cosa B, Moar W, Lee S-B, Miller M, Daniell H (2001) Overexpression of Bt Cry2Aa2 operon in chloroplasts leads to formation of insecticidal crystals. Nat Biotechnol 19:71–74
de Vetten N, Wolters A-M, Raemakers K, van der Meer I, ter Stege R, Heeres E, Heeres P, Visser R (2003) A transformation method for obtaining marker-free plants of a cross-pollinating and vegetatively propagated crop. Nat Biotechnol 21:439–442
Desfeux C, Clough SJ, Bent AF (2000) Female reproductive tissues are the primary target of Agrobacterium-mediated transformation by the Arabidopsis floral-dip method. Plant Physiol 123:895–904
Doran PM (2006) Foreign protein degradation and instability in plants and plant tissue cultures. Trends Biotechnol 24:426–432
Engelen-Eigles G, Erwin JE (1997) A model plant for vernalization studies. Sci Hortic 70:197–202
Feldmann KA, Marks MD (1987) Agrobacterium-mediated transformation of germinating seeds of Arabidopsis thaliana: a non-tissue culture approach. Mol Gen Genet 208:1–9
Fowler S, Lee K, Onouchi H, Samach A, Richardson K, Coupland G, Putterill J (1999) GIGANTEA: a circadian clock-controlled gene that regulates photoperiodic flowering in Arabidopsis and encodes a protein with several possible membrane-spanning domains. EMBO J 18:4679–4688
Guéritaine G, Darmency H (2001) Polymorphism for interspecific hybridization between oilseed rape (Brassica napus) and a population of wild radish (Raphanus raphanistrum). Sex Plant Reprod 14:169–172
Guéritaine G, Bonavent JF, Darmency F (2003) Variation of prezygotic barriers in the interspecific hybridization between oilseed rape and wild radish. Euphytica 130:349–353
Hou B-K, Zhou Y-H, Wan L-H, Zhang Z-L, Shen G-F, Chen Z-H, Hu Z-M (2003) Chloroplast transformation in oilseed rape. Transgenic Res 12:111–114
Huh MK, Ohnishi O (2002) Genetic diversity and genetic relationships of East Asian natural populations of wild radish revealed by AFLP. Breed Sci 52:79–88
Huq E, Tepperman JM, Quail PH (2000) GIGANTEA is a nuclear protein involved in phytochrome signaling in Arabidopsis. Proc Natl Acad Sci USA 97:9789–9794
Jeong WJ, Min SR, Liu JR (1995) Somatic embryogenesis and plant regeneration in tissue cultures of radish (Raphanus sativus L.). Plant Cell Rep 14:648–651
Johanson U, West J, Lister C, Michaels S, Amasino R, Dean C (2000) Molecular analysis of FRIGIDA, a major determinant of natural variation in Arabidopsis flowering time. Science 290:344–347
Katavic V, Haughn GW, Reed D, Martin M, Kunst L (1994) In planta transformation of Arabidopsis thaliana. Mol Gen Genet 245:363–370
Kaymak HÇ, Güvenç I (2010) The influence of vernalization time and day length on flower induction of radish (Raphanus sativus L.) under controlled and field conditions. Turk J Agric For 34:401–413
Kerlan M, Chevre A, Eber F, Baranger A, Renard M (1992) Risk assessment of outcrossing of transgenic rapeseed to related species. I. Interspecific hybrid production under optimal conditions with emphasis on pollination and fertilization. Euphytica 62:145–153
Kilby NJ, Davies GJ, Michael RS, Murray JAH (1995) FLP recombinase in transgenic plants: constitutive activity in stably transformed tobacco and generation of marked cell clones in Arabidopsis. Plant J 8:637–652
Kong Q, Li X, Xiang C, Wang H, Song J, Zhi H (2010) Genetic diversity of radish (Raphanus sativus L.) germplasm resources revealed by AFLP and RAPD markers. Plant Mol Biol Rep. doi:10.1007/s11105-010-0228-7
Koornneef M, van Eden J, Hanhart CJ, Stam P, Braaksma FJ, Feenstra WJ (1983) Linkage map of Arabidopsis thaliana. J Hered 74:265–272
Koornneef M, Hanhart CJ, Van Der Veen JH (1991) A genetic and physiological analysis of late flowering mutants in Arabidopsis thaliana. Mol Gen Genet 229:57–66
Kraus (2010) Concepts of marker genes for plants. In: Kempken F, Jungs C (eds) Genetic modification of plants, Biotechnology in agriculture and forestry, vol 64. Springer, Heidelberg, pp 39–60
Kumar S, Allen GC, Thompson WF (2006) Gene targeting in plants: fingers on the move. Trends Plant Sci 11:159–161
Lazo GR, Stein PA, Ludwig RA (1991) A DNA transformation-competent Arabidopsis genomic library in Agrobacterium. Biotechnology 9:963–967
Lee S-S (1987) Bolting in radish. In: Asian and Pacific Council (eds) Improved vegetable production in Asia. Food & Fertilizer Technology Center, Taipei, Series No. 36, pp 60–70
Lee M, Phillips RL (1988) The chromosomal basis of somaclonal variation. Annu Rev Plant Physiol Plant Mol Biol 39:413–437
Leroux B, Carmoy N, Giraudet D, Potin P, Larher F, Bodin M (2009) Inhibition of ethylene biosynthesis enhances embryogenesis of cultured microspores of Brassica napus. Plant Biotechnol Rep 3:347–353
Levy YY, Mesnage S, Mylne JS, Gendall AR, Dean C (2002) Multiple roles of Arabidopsis VRN1 in vernalization and flowering time control. Science 297:243–246
Li B, Xie C, Qiu H (2009) Production of selectable marker-free transgenic tobacco plants using a non-selection approach: chimerism or escape, transgene inheritance, and efficiency. Plant Cell Rep 28:373–386
Lichter R (1989) Efficient yield of embryoids by culture of isolated microspores of different Brassiaceae species. Plant Breed 103:119–123
Lico C, Chen Q, Santi L (2008) Viral vectors for production of recombinant proteins in plants. J Cell Physiol 216:366–377
Liu LW, Zhao LP, Gong YQ, Wang MX, Chen LM, Yang JL, Wang Y, Yu FM, Wang LZ (2008) DNA fingerprinting and genetic diversity analysis of late-bolting radish cultivars with RAPD, ISSR and SRAP markers. Sci Hortic 116:240–247
Lu N, Yamane K, Ohnishi O (2008) Genetic diversity of cultivated and wild radish and phylogenetic relationships among Raphanus and Brassica species revealed by the analysis of trnK/matK sequence. Breed Sci 58:15–22
Lucca P, Ye X, Potrykus I (2001) Effective selection and regeneration of transgenic rice plants with mannose as selective agent. Mol Breed 7:43–49
Madou P, Wells A, Pang ECK, Stevenson TW (2005) Genetic variation in populations of Western Australian wild radish. Aust J Agric Res 56:1079–1087
Malnoy M, Borejsza-Wysocka EE, Abbott P, Lewis S, Norelli JL, Flaishman M, Gidoni D, Aldwinckle HS (2007) Genetic transformation of apple without use of a selectable marker. Acta Hortic 738:319–322
Marillonnet S, Giritch A, Gils M, Kandzia R, Klimyuk V, Gleba Y (2004) In planta engineering of viral RNA replicons: efficient assembly by recombination of DNA modules delivered by Agrobacterium. Proc Natl Acad Sci USA 101:6852–6857
Marillonnet S, Thoeringer C, Kandzia R, Klimyuk V, Gleba Y (2005) Systemic Agrobacterium tumefaciens-mediated transfection of viral replicons for efficient transient expression in plants. Nat Biotechnol 23:718–723
Matsubura S, Hegazi HH (1990) Plant regeneration from hypocotyl callus of radish. Hortic Sci 25:1286–1288
Michaels SD, Amasino RM (1999) FLOWERING LOCUS C encodes a novel MADS domain protein that acts as a repressor of flowering. Plant Cell 11:445–458
Muminovic J, Merz A, Melchinger AE, Lubberstedt T (2005) Genetic structure and diversity among radish varieties as inferred from AFLP and ISSR analyses. J Am Soc Hortic Sci 130:79–87
Nadkarni KM (1927) Dr. K. M. Nadkarni’s Indian Material Medica. Popular Prakashan, Bombay
Nakamura Y, Iwahashi T, Tanaka A, Koutani J, Matsuo T, Okamoto S, Sato K, Ohtsuki K (2001) 4-(Methylthio)-3-butenyl isothiocyanate, a principal antimutagen in daikon (Raphanus sativus; Japanese white radish). J Agric Food Chem 49:5755–5760
Nguyen TT, Nugent G, Cardi T, Dix PJ (2005) Generation of homoplasmic plastid transformants of a commercial cultivar of potato (Solanum tuberosum L.). Plant Sci 168:1495–1500
Ogura H (1968) Studies on the new male sterility in Japanese radish with special reference to utilization of this sterility towards the practical raising of hybrid seeds. Mem Fac Agric Kagoshima Univ 6:39–78
Park DH, Somers DE, Kim YH, Choy YH, Lim HK, Soh MS, Kim HJ, Kay SA, Nam HG (1999) Control of circadian rhythms and photoperiodic flowering by the Arabidopsis GIGANTEA gene. Science 285:1579–1582
Park B-J, Liu Z, Kanno A, Kameya T (2005) Transformation of radish (Raphanus sativus L.) via sonication and vacuum infiltration of germinated seeds with Agrobacterium harbouring a group 3 LEA gene from B. napus. Plant Cell Rep 24:494–500
Pua E-C, Lee JEE (1995) Enhanced de novo shoot morphogenesis in vitro by expression of antisense 1-aminocyclopropane-1-carboxylate oxidase gene in transgenic mustard plants. Planta 196:69–76
Pua E-C, Sim G-E, Chi G-L, Kong L-F (1996) Synergistic effect of ethylene inhibitors and putrescine on shoot regeneration from hypocotyls explants of Chinese radish (Raphanus sativus L. var. longipinnatus Bailey) in vitro. Plant Cell Rep 15:685–690
Qing CM, Fan L, Lei Y, Bouchez D, Tourneur C, Yan L, Robaglia C (2000) Transformation of Pakchoi (Brassica rapa L. ssp. chinensis) by Agrobacterium infiltration. Mol Breed 6:67–72
Quisumbing E (1951) Medicinal plants of the Philippines. In: Technical bulletin no. 16. Republic of the Philippines Department of Agriculture and Natural Resources, Manilla Bureau of Printing
Redei GP (1962) Supervital mutants of Arabidopsis. Genet 47:443–460
Richardson K, Fowler S, Pullen C, Skelton C, Morris B, Putterill J (1998) T-DNA tagging of a flowering-time gene and improved gene transfer by in planta transformation of Arabidopsis. Aust J Plant Physiol 25:125–130
Ridley CE, Ellstrand NC (2009) Evolution of enhanced reproduction in the hybrid-derived invasive, California wild radish (Raphanus sativus). Biol Invasions 11:2251–2264
Rieger MA, Potter TD, Preston C, Powles SB (2001) Hybridisation between Brassica napus L. and Raphanus raphanistrum L. under agronomic field conditions. Theor Appl Genet 103:555–560
Roggen HPJR, Van Dijk AJ (1973) Electric aided and bud pollination. Euphytica 22:260–263
Ruf S, Hermann M, Berger IJ, Carrer H, Bock R (2001) Stable genetic transformation of tomato plastids and expression of a foreign protein in fruit. Nat Biotechnol 19:870–875
Sikdar S, Seriono G, Chaudhuri S, Maliga P (1998) Plastid transformation in Arabidopsis thaliana. Plant Cell Rep 18:20–25
Simpson GG, Dean C (2002) Arabidopsis, the Rosetta Stone of flowering time? Science 296:285–289
Sivamani E, Bahieldin A, Wraith JM, Al-Niemi T, Dyer WE, Ho THD, Qu R (2000) Improved biomass productivity and water use efficiency under water deficit conditions in transgenic wheat constitutively expressing the barley HVA1 gene. Plant Sci 155:1–9
Skarjinskaia M, Svab Z, Maliga P (2003) Plastid transformation in Lesquerella fendleri, an oilseed Brassicacea. Transgenic Res 12:115–122
Sparrow PA, Irwin JA, Dale PJ, Twyman RM, Ma JK (2007) Pharma-planta: road testing the developing regulatory guidelines for plant-made pharmaceuticals. Transgenic Res 16:147–161
Staub JM, Maliga (1992) Long regions of homologous DNA are incorporated into the tobacco plastid genome by transformation. Plant Cell 4:39–45
Steinitz B, Barr N, Tabib Y, Vaknin Y, Bernstein N (2010) Control of in vitro rooting and plant development in Corymbia maculata by silver nitrate, silver thiosulfate and thiosulfate ion. Plant Cell Rep 29:1315–1323
Sugita K, Kasahara T, Matsunaga E, Ebinuma H (2000) A transformation vector for the production of marker-free transgenic plants containing a single copy transgene at high frequency. Plant J 22:461–469
Svab Z, Maliga P (1993) High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene. Proc Natl Acad Sci USA 90:913–917
Swire-Clark GA, Marcotte WR Jr (1999) The wheat LEA protein Em functions as an osmoprotective molecule in Saccharomyces cerevisiae. Plant Mol Biol 39:117–128
Takahata Y, Komatsu H, Kaizuma N (1996) Microspore culture of radish (Raphanus sativus L.): influence of genotype and culture conditions on embryogenesis. Plant Cell Rep 16:163–166
Trieu AT, Burleigh SH, Kardailsky IV, Maldonado-Mendoza IE, Versaw WK, Blaylock LA, Shin H, Chiou T-J, Katagi H, Dewbre GR, Weigel D, Harrison M (2000) Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium. Plant J 22:531–541
Wang K (2006) Methods in Molecular Biology, vol 343: Agrobacterium protocols, 2nd edn, vol 1. Humana Press Inc, Totowa
Wang L, Wei L, Wang L, Xu C (2002) Effects of peroxidase on hyperlipidemia in mice. J Agric Food Chem 50:868–870
Yamagishi H (2004) Assessment of cytoplasmic polymorphisms by PCR-RFLP of the mitochondrial orfB region in wild and cultivated radishes (Raphanus). Plant Breed 123:141–144
Yamagishi H, Tateishi M, Terachi T, Murayama S (1998) Genetic relationships among Japanese wild radishes (Raphanus sativus f. raphanistroides Makino), cultivated radishes and R. raphanistrum revealed by RAPD analysis. J Jpn Soc Hortic Sci 67:526–531
Yamane K, Lu N, Ohnishi O (2005) Chloroplast DNA variations of cultivated radish and its wild relatives. Plant Sci 168:627–634
Yan Z, Liang D, Liu H, Zheng G (2010) FLC: a key regulator of flowering time in Arabidopsis. Russ J Plant Physiol 57:166–174
Ye GN, Stone G, Pang SZ, Creely W, Gonzalez K, Hinchee M (1999) Arabidopsis ovule is the target for Agrobacterium in planta vacuum infiltration transformation. Plant J 19:249–257
Zuo J, Niu Q-W, Chua N-H (2000) An estrogen receptor-based transactivator XVE mediates highly inducible gene expression in transgenic plants. Plant J 24:265–273
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Communicated by R. Reski.
This work is dedicated to my loving mother Brenda Curtis (1931–2008), my number one fan. Rest in peace.
A contribution to the Special Issue: Plant Biotechnology in Support of the Millennium Development Goals.
An erratum to this article can be found at http://dx.doi.org/10.1007/s00299-011-1090-2
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Curtis, I.S. Genetic engineering of radish: current achievements and future goals. Plant Cell Rep 30, 733–744 (2011). https://doi.org/10.1007/s00299-010-0978-6
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DOI: https://doi.org/10.1007/s00299-010-0978-6