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Current trends and future prospects of biotechnological interventions through tissue culture in apple

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Abstract

Apple (Malus domestica Borkh.), which is a widely cultivated, important economic fruit crop with nutritive and medicinal importance, has emerged as a model horticultural crop in this post-genomic era. Apple cultivation is heavily dependent on climatic condition and is susceptible to several diseases caused by fungi, bacteria, viruses, insects, etc. Extensive research work has been carried out to standardize tissue culture protocols and utilize them in apple improvement. We review the in vitro shoot multiplication, rooting, transformation and regeneration methodologies in apple and tabulate various such protocols for easy reference. The utility and limitation of transgenesis in apple improvement have also been summarized. The concepts of marker-free plants, use of non-antibiotic resistance selectable markers, and cisgenic and intragenic approaches are highlighted. Furthermore, the limitations, current trends and future prospects of tissue culture-mediated biotechnological interventions in apple improvement are discussed.

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Abbreviations

BAP:

6-Benzylaminopurine

IBA:

Indole-3-butyric acid

IAA:

Indole-3-acetic acid

GA:

Gibbrellic acid

KN:

Kinetin

TDZ:

Thidiazuron

NAA:

1-Naphthyl acetic acid

2,4-D:

2,4-Dichlorophenoxy acetic acid

ABA:

Abscisic acid

TIBA:

2,3,5-Triiodobenzoic acid

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Acknowledgments

SB was supported by a research fellowship from the University Grants Commission, Govt of India. The inputs and suggestions given by PS Ahuja during manuscript preparation are acknowledged. The work has been supported by research funding from CSIR. Due to space constraints, we have not been able to cite the valuable work of several researchers in this review. IHBT publication number: 2046.

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  1. Biotechnology Division, Institute of Himalayan Bioresource Technology, Council of Scientific and Industrial Research, Palampur, 176061, Himachal Pradesh, India

    Shammi Bhatti & Gopaljee Jha

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  1. Shammi Bhatti
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  2. Gopaljee Jha
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Correspondence to Gopaljee Jha.

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Communicated by R. Reski.

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Bhatti, S., Jha, G. Current trends and future prospects of biotechnological interventions through tissue culture in apple. Plant Cell Rep 29, 1215–1225 (2010). https://doi.org/10.1007/s00299-010-0907-8

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