Abstract
A new reliable protocol for the induction of adventitious shoot formation and plant regeneration from apple callus has been developed. High regeneration frequency was obtained with this method in four different genotypes (Jork9, M26, Gala and McIntosh) and callus maintained regeneration ability for several months. The procedure consists of inducing vegetative shoot apices, excised from in vitro shoots, for 20 days in darkness on an MS medium without glycine, supplied with 17.8 μM BA, 2.7 μM NAA and 250 mg l−1 cefotaxime. The explants are then transferred to a fresh auxin-free medium and given light. Histological studies revealed that all the regenerated shoots originated from callus. Regenerated shoots were multiplied, rooted and successfully established in soil.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 2 April 1999 / Revision received: 10 November 1999 / Accepted: 15 November 1999
Rights and permissions
About this article
Cite this article
Caboni, E., Lauri, P. & D'Angeli, S. In vitro plant regeneration from callus of shoot apices in apple shoot culture. Plant Cell Reports 19, 755–760 (2000). https://doi.org/10.1007/s002999900189
Issue Date:
DOI: https://doi.org/10.1007/s002999900189