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Differential expression analysis by cDNA-AFLP of Saccharum spp. after inoculation with the host pathogen Sporisorium scitamineum

  • Physiology and Biochemistry
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Abstract

A differential expression study on the Saccharum spp.–Sporisorium scitamineum pathogenic interaction was carried out in the susceptible Ja60-5 and the resistant M31/45 genotypes. Using cDNA-AFLP analysis, a total of 64 transcript-derived fragments (TDFs) was found to be differentially expressed, with the majority (67.2%) of the differential TDFs up-regulated in the resistant M31/45 cultivar. The plant response against S. scitamineum infection was complex, representing major genes involved in oxidative burst, defensive response, ethylene and auxins pathways during the first 72 h post-inoculation. Results obtained suggest a key role for genes involved in the oxidative burst and the lignin pathways in the initial sugarcane defense against the S. scitamineum infection.

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Acknowledgments

To Barbara Randolph-Anderson PhD (Minneapolis, USA) and Maribel Alvarez PhD (University of Arizona, USA) for improve the language and the manuscript revision. Thank to the anonymous referees for their useful critical.

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Authors and Affiliations

  1. National Institute for Sugarcane Research, CUJAE Km 2 1/2 Road. Boyeros, 19390, Havana City, Cuba

    María LaO, Ariel D. Arencibia, Elva R. Carmona, Ricardo Acevedo, Eida Rodríguez & Ignacio Santana

  2. National Center for Animal and Plant Health, P.O.Box 10, San Jose de Las Lajas, Havana, Cuba

    María LaO & Ondina León

Authors
  1. María LaO
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  2. Ariel D. Arencibia
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  3. Elva R. Carmona
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  4. Ricardo Acevedo
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  5. Eida Rodríguez
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  6. Ondina León
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  7. Ignacio Santana
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Corresponding author

Correspondence to Ariel D. Arencibia.

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Communicated by P. Lakshmanan.

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LaO, M., Arencibia, A.D., Carmona, E.R. et al. Differential expression analysis by cDNA-AFLP of Saccharum spp. after inoculation with the host pathogen Sporisorium scitamineum . Plant Cell Rep 27, 1103–1111 (2008). https://doi.org/10.1007/s00299-008-0524-y

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  • DOI: https://doi.org/10.1007/s00299-008-0524-y

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