Summary
Potato tuber life-cycle is composed of many individual developmental stages including tuber formation, tuber development, dormancy and sprouting. We have used cDNA-AFLP fingerprinting to analyse gene expression in 24 individual stages of development, over the period from stolon formation through sprouting. In addition to these developmental stages, different tissues were analysed to assess tissue specificity and various controls were incorporated to determine process specificity. In total around 18000 transcript derived cDNA fragments (TDFs) were visualised from which circa 2600 were included in a statistical analysis allowing general conclusions about gene expression during development. More than 200 process specific TDFs were isolated and sequenced throughout the potato tuber life-cycle. The sequence similarities of these TDFs to known genes give an insight into the kinds of processes occurring during tuberisation, dormancy and sprouting.
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References
Altschul, S.F., T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller & D.J. Lipman, 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.Nucleic Acids Research 25 (17): 3389–3402.
Appeldoorn, N.J.G., S.M. de Bruijn, E.A.M. Koot-Gronsveld, R.G.F. Visser, D. Vreugdenhil & L.H.W. van der Plas, 1999. Developmental changes in enzymes involved in the conversion of hexose phosphate and its subsequent metabolites during early tuberization of potato.Plant, Cell and Environment 22: 1085–1096.
Bachem, C.W.B., R.S. van der Hoeven, S.M. de Bruijn, D. Vreugdenhil, M. Zabeau & R. Visser, 1996. Visualisation of differential gene expression using a novel method of RNA finger-printing based on AFLP: Analysis of gene expression during potato tuber development.Plant Journal 9: 745–753.
Bachem, C.W.B., R.J.F.J. Oomen & R.G.F. Visser, 1998. Transcript imaging with cDNA-AFLP: A step-by-step protocol.Plant Molecular Biology Reporter 16: 157–173.
Bergey, D.R., G.A. Howe & C.A. Ryan, 1996. Polypeptide signaling for plant defensive genes exhibits analogies to defense signaling in animals.Proceedings of the National Academy of Sciences of the USA 93: 12053–12058.
Biemelt, Sophia, Mohammad Hajirezaei, Eike Hentschel and Uwe Sonnewald, 2000. Comparative analysis of abscisic acid content and starch degradation during storage of tubers harvested from different potato varieties.Potato Research 43: 371–382.
Burton, W.G., 1989. The Potato. 3rd edition. Longman Scientific & Technical, New York.
Carrera, E., S.D. Jackson & S. Prat, 1999. Feedback control and diurnal regulation of gibberellin 20-oxidase transcript levels in potato.Plant Physiology 119: 765–774.
Chory, J. & J. Li, 1997. Gibberellins, brassinosteroids and light-regulated development.Plant, Cell and Environment 20: 801–806.
Ittersum, M.K. van, 1992. Dormancy and growth vigour of seed potatoes. PhD thesis, Wageningen Agricultural University, Wageningen, pp 187.
Jackson, S.D., P. James, S. Prat & B. Thomas, 1998. Phytochrome B affects the levels of a graft-transmissible signal involved in tuberization.Plant Physiology 117: 29–32.
Kamiya, Y. & J.L. Garcia-Martinez, 1999. Regulation of gibberellin biosynthesis by light.Current Opinion in Plant Biology 2: 398–403.
Kitajima, S. & F. Sato, 1999. Plant pathogenesis-related proteins: molecular mechanisms of gene expression and protein function.The Journal of Biochemistry (Tokyo) 125: 1–8.
Koda, Y., E.A. Omer, T. Yoshihara, H. Shibata, S. Sakamura & Y. Okazawa, 1988. Isolation of a specific potato tuber-inducing substance from potato leaves.Plant and Cell Physiology 29: 1047–1051.
Lin, X., S. Kaul, S. Rounsley, T.P. Shea, M.I. Benito, C.D. Town, C.Y. Fujii, T. Mason, C.L. Bowman, M. Barnstead, T.V. Feldblyum, C.R. Buell, K.A. Ketchum, J. Lee, C.M. Ronning, H.L. Koo, K.S. Moffat, L.A. Cronin, M. Shen, G. Pai, S. van Aken, L. Umayam, L.J. Tallon, J.E. Gill, J.C. Venter et al., 1999. Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.Nature 402 (6763): 761–768.
Park, W.D., D.J. Hannapel, G. Mignery & C.S. Pikaard, 1985. Molecular approaches to the study of the major tuber proteins. In: P.H. Li (Ed.), Potato Physiology. Academic Press Inc., Orlando, Florida.
Rholf, F.J., 1993. NTsys PC version 2.02. Applied Biostatistics, New York.
Sanz, M.J., A. Mingo-Castel, A.A.M. van Lammeren & D. Vreugdenhil, 1996. Changes in the microtubular cytoskeleton precede in vitro tuber formation in potato.Protoplasma 191: 46–54.
Xu, X., A.A.M. van Lammeren, E. Vermeer & D. Vreugdenhil, 1998. The role of gibberellin, abscisic acid, and sucrose in the regulation of potato tuber formation in vitro.Plant Physiology 117: 575–584.
Xu, Y.L., L. Li, D.A. Gage & J.A. Zeevaart, 1999. Feedback regulation of GA5 expression and metabolic engineering of gibberellin levels in Arabidopsis.Plant Cell 11: 927–936.
Yamaguchi, S., M.W. Smith, R.G. Brown, Y. Kamiya & T. Sun, 1998. Phytochrome regulation and differential expression of gibberellin 3beta-hydroxylase genes in germinating Arabidopsis seeds.Plant Cell 10: 2115–2126.
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Bachem, C., van der Hoeven, R., Lucker, J. et al. Functional genomic analysis of potato tuber life-cycle. Potato Res 43, 297–312 (2000). https://doi.org/10.1007/BF02360536
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DOI: https://doi.org/10.1007/BF02360536