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Exogenous and endogenous growth regulators on apogamy in Dryopteris affinis (Lowe) Fraser-Jenkins sp. affinis

  • Cell Biology and Morphogenesis
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Abstract

This work showed for the first time the relationship between the effect of exogenous auxins and gibberellins on apogamy in Dryopteris affinis (Lowe) Fraser-Jenkins sp. affinis and its endogenous contents during early apogamic events. The addition of NAA (0.53 and 5.37 μM) or GA3 2.8 μM to an MS solid medium significantly increased apogamous sporophyte formation. BA induced brown callus that regenerated sporophytes in a hormone-free medium. The endogenous contents of GA1, GA3, GA4, GA7, GA9 and IAA were determined by GC–MS in gametophytes cultured on MS solid medium, before and during early stages of apogamous embryo development. The accumulation of both GA9 and IAA before embryo development was evident as high levels of GA4 in the earliest analysed stage of embryo development and high levels of GA3 in elongating shoots were found. The role of gibberellins on apogamy was also supported by data showing a decrease in the percentage of gametophytes developing embryos because of the addition of flurprimidol to the culture medium.

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Abbreviations

BA:

6-Benzyladenine

GA3 :

Gibberellic acid

DW:

Dry weight

GC–MS:

Gas chromatography–mass spectrometry

IAA:

Indole-3-acetic acid

m/z :

Mass to charge ratio

MS medium:

Murashige and Skoog mineral medium

NAA:

Naphtaleneacetic acid

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Authors and Affiliations

  1. Area de Fisiología Vegetal, Dpt. B.O.S., c/ Catedrático R Uría, s/n, 33071, Oviedo, Spain

    V. Menéndez, N. F. Villacorta, M. A. Revilla & H. Fernández

  2. Dpt. Química Orgánica e Inorgánica, c/ Julián Clavería, s/n, 33071, Oviedo, Spain

    V. Gotor & P. Bernard

Authors
  1. V. Menéndez
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  2. N. F. Villacorta
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  3. M. A. Revilla
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  4. V. Gotor
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  5. P. Bernard
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  6. H. Fernández
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Correspondence to H. Fernández.

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Communicated by H. Lörz

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Menéndez, V., Villacorta, N.F., Revilla, M.A. et al. Exogenous and endogenous growth regulators on apogamy in Dryopteris affinis (Lowe) Fraser-Jenkins sp. affinis . Plant Cell Rep 25, 85–91 (2006). https://doi.org/10.1007/s00299-005-0041-1

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  • DOI: https://doi.org/10.1007/s00299-005-0041-1

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