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IAA-induced apogamy in Platycerium coronarium (Koenig) Desv. gametophytes cultured in vitro

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Abstract

Apogamous sporophytes were produced on Platycerium coronarium gametophytes cultured in the presence of indole-3-acetic acid (IAA). The percentage of apogamy as well as the total number of apogamous sporophytes produced per gametophyte clump were highest in the presence of 40 μM IAA. When ethylene was allowed to accumulate in the culture vessel in the presence of an optimum level of IAA, the percentage and total number of apogamous sporophyte production decreased significantly. Using light microscope and confocal laser scanning microscope we have shown that nuclear size can be used as a quick parameter to estimate the ploidy level of P. coronarium.

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Abbreviations

CLSM:

confocal laser scanning microscope

IAA:

Indole-3-acetic acid

MS:

Murashige and Skoog

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Authors and Affiliations

  1. Department of Botany, National University of Singapore, Lower Kent Ridge Road, 0511, Singapore

    Siew-Hwa Kwa, Yeow-Chin Wee & Prakash P. Kumar

  2. Department of Zoology, National University of Singapore, Lower Kent Ridge Road, 0511, Singapore

    Tit-Meng Lim

Authors
  1. Siew-Hwa Kwa
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  2. Yeow-Chin Wee
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  3. Tit-Meng Lim
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  4. Prakash P. Kumar
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Communicated by A. Komamine

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Kwa, SH., Wee, YC., Lim, TM. et al. IAA-induced apogamy in Platycerium coronarium (Koenig) Desv. gametophytes cultured in vitro . Plant Cell Reports 14, 598–602 (1995). https://doi.org/10.1007/BF00231946

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  • DOI: https://doi.org/10.1007/BF00231946

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