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Effect of zearalenone treatment on the production of wheat haploids via the maize pollination system

  • Cell Morphology and Morphogenesis
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Abstract

The ability of zearalenone (ZEN) to stimulate the growth of haploid wheat embryos formed following the pollination of wheat spikes with maize pollen was tested. The maize pollination system was used as a model to compare the activity of ZEN with that of auxin analogues. Three solutions, each with a different concentration of ZEN (6.0, 3.0 or 1.5 μM), and a solution of 2,4-dichlorophenoxyacetic acid (control) were tested for their effect on ovary swelling, frequency of embryo formation and the ability to regenerate plants. In total, 3,105 florets of 282 spikes from five different cultivars of hexaploid winter wheat (Triticum aestivum L.) were pollinated with maize (Zea mays L. cv. Gama) pollen and treated with the ZEN solutions. The highest concentration of ZEN (6.0 μM) was the most effective in inducing ovary swelling (84 swollen ovaries/100 pollinated florets) and increasing the frequency of embryo induction (18.9 embryos/100 pollinated florets), but these embryos were severely deformed. They had low capability to germinate in vitro, while callus was easily formed and indirect regeneration of plants was possible. The lowest ZEN concentration (1.5 μM) induced ovary swelling in 42.8/100 pollinated florets and embryo growth in 3.3 out of 100 pollinated florets. The embryos were regular in shape, and almost half of them germinated in vitro while callus induction from them failed. The concentration of 3 μM ZEN had an intermediate effect. The type of response of the various wheat genotypes was similar, while frequencies were different, with cv. Izolda being the most responsive. The results show that ZEN has some of the properties of an auxin analogue, while other effects of its action are unique. It can be used in the maize pollination system of doubled haploid production to replace auxin analogues when indirect regeneration of plants via callus tissue is planned.

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Authors and Affiliations

  1. Department of Plant Physiology, Polish Academy of Sciences, Cracow, Poland

    J. Biesaga-Kościelniak, I. Marcińska & M. Wędzony

  2. Chair of Plant Physiology, Faculty of Agriculture, University of Agriculture, Cracow, Poland

    J. Kościelniak

Authors
  1. J. Biesaga-Kościelniak
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  2. I. Marcińska
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  3. M. Wędzony
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  4. J. Kościelniak
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Correspondence to J. Kościelniak.

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Communicated by K. Glimelius

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Biesaga-Kościelniak, J., Marcińska, I., Wędzony, M. et al. Effect of zearalenone treatment on the production of wheat haploids via the maize pollination system. Plant Cell Rep 21, 1035–1039 (2003). https://doi.org/10.1007/s00299-003-0622-9

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  • DOI: https://doi.org/10.1007/s00299-003-0622-9

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