Abstract
Lactobacillus buchneri strain WPZ001 that could efficiently produce gamma-aminobutyric acid was isolated from Chinese fermented sausages. Optimal cultivation conditions for gamma-aminobutyric acid production in L. buchneri WPZ001 were determined, and xylose was found to be the best carbon source. Using xylose as the sole carbon source, 70 g/L gamma-aminobutyric acid was produced by flask fermentation of L. buchneri WPZ001 for 48 h, and the harvested cells could continue to convert monosodium glutamate to gamma-aminobutyric acid in buffer and produce 59 g gamma-aminobutyric acid after eight runs of biotransformation; the total yield of gamma-aminobutyric acid reached 129 g/L. This combination strategy also worked well when the low-cost corncob hydrolysate was used as the sole carbon source, and the yield of gamma-aminobutyric acid reached 117 g/L. The results indicate that L. buchneri WPZ001 has great potential for industrial production of gamma-aminobutyric acid.
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Funding was provided by grants from the National Key Basic Research Program of China (973 Program 2012CB725202), National Natural Science Foundation of China (NSFC31370131), Six Talent Peaks Project of Jiangsu Province (2012-SWYY-008), and Key Research Project of Jiangnan University (JUSRP51303A).
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Zhao, A., Hu, X., Pan, L. et al. Isolation and characterization of a gamma-aminobutyric acid producing strain Lactobacillus buchneri WPZ001 that could efficiently utilize xylose and corncob hydrolysate. Appl Microbiol Biotechnol 99, 3191–3200 (2015). https://doi.org/10.1007/s00253-014-6294-2
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DOI: https://doi.org/10.1007/s00253-014-6294-2