Abstract
Lactobacillus buchneri strain NRRL B-30929 was isolated from a fuel ethanol production facility. This heterofermentative, facultative anaerobe can utilize xylose as a sole carbon source and tolerates up to 12% ethanol. Carbohydrate utilization (API, Biomerieux) and Phenotype Microarrays™ (PM, Biolog) analyses indicated that the strain is able to metabolize a broad spectrum of carbon sources including various monosaccharides (C5 and C6), disaccharides and oligosaccharides, with better rates under anaerobic conditions. In pH-controlled bioreactors, the bacterium consumed xylose and glucose simultaneously at high concentrations (125 g L−1, pH 6.0). The major fermentation products were lactate (52 g L−1), acetate (26 g L−1) and ethanol (12 g L−1). The strain ferments glucose alone (pH 4.0) into lactate and ethanol with a molar ratio of 1.03:1. This strain will be further explored via genetic engineering for potential applications in biomass conversion.
Similar content being viewed by others
References
Adesogan AT, Salawu MB, Ross AB, Davies DR, Brooks AE (2003) Effect of Lactobacillus buchneri, Lactobacillus fermentum, Leuconostoc mesenteroides inoculants, or a chemical additive on the fermentation, aerobic stability, and nutritive value of crimped wheat grains. J Dairy Sci 86:1789–1796
Contreras-Govea F, Albrecht K, Muck RE (2006) Spring yield and silage characteristics of Kura clover, winter wheat, and in mixtures. Agron J 98:781–787
Dien BS, Nichols NN, Bothast RJ (2001) Recombinant Escherichia coli engineered for production of l-lactic acid from hexose and pentose sugars. J Ind Microbiol Biotechnol 27:259–264
Gold RS, Meagher MM, Hutkins R, Conway T (1992) Ethanol tolerance and carbohydrate metabolism in lactobacilli. J Ind Microbiol 10:45–54
Hugenholtz J (1993) Citrate metabolism in lactic acid bacteria. FEMS Microbiol Rev 12:165–178
Iyer PV, Thomas S, Lee YY (2000) High-yield fermentation of pentoses into lactic acid. Appl Biochem Biotechnol 84–86:665–777
Keiichi K (1976) Purification of Lactobacillus buchneri glucose 6-phosphate dehydrogenase by affinity chromatography on blue dextran-sepharose. Fac Agric 52:128–130
Kleinschmit DH, Schmidt RJ, Kung L Jr (2005) The effects of various antifungal additives on the fermentation and aerobic stability of corn silage. J Dairy Sci 88:2130–2139
Krooneman J, Faber F, Alderkamp AC, Elferink SJ, Driehuis F (2002) Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage. Int J Syst Evol Microbiol 52:639–646
Kung L Jr, Ranjit NK (2001) The effect of Lactobacillus buchneri and other additives on the fermentation and aerobic stability of barley silage. J Dairy Sci 84:1149–1155
Kung L Jr, Taylor CC, Lynch MP, Neylon JM (2003) The effect of treating alfalfa with Lactobacillus buchneri 40788 on silage fermentation, aerobic stability, and nutritive value for lactating dairy cows. J Dairy Sci 86:336–343
Liu S, Saha B, Cotta M (2005) Cloning, expression, purification, and analysis of mannitol dehydrogenase gene mtlK from Lactobacillus brevis. Appl Biochem Biotechnol 121–124:391–401
Maas RH, Bakker RR, Eggink G, Weusthuis RA (2006) Lactic acid production from xylose by the fungus Rhizopus oryzae. Appl Microbiol Biotechnol 72:861–868
Makarova K, Slesarev A, Wolf Y, Sorokin A, Mirkin B (2006) Comparative genomics of the lactic acid bacteria. Proc Natl Acad Sci USA 103:15611–15616
Matte A, Forsberg CW, Verrinder Gibbins AM (1992) Enzymes associated with metabolism of xylose and other pentoses by Prevotella ruminicola strains, Selenomonas ruminantium D, and Fibrobacter succinogenes S85. Can J Microbiol 38:370–376
Mills DA, Rawsthorne H, Parker C, Tamir D, Makarova K (2005) Genomic analysis of Oenococcus oeni PSU-1 and its relevance to winemaking. FEMS Microbiol Rev 29:465–475
Mital BK, Shallenberger RS, Steinkraus KH (1973) α-galactosidase activity of lactobacilli. Appl Microbiol 26:783–788
Muck RE (2004) Effects of corn silage inoculants on aerobic stability. Trans ASAE 47:1011–1016
Nishino N, Yoshida M, Shiota H, Sakaguchi E (2003) Accumulation of 1,2-propanediol and enhancement of aerobic stability in whole crop maize silage inoculated with Lactobacillus buchneri. J Appl Microbiol 94:800–807
Nsereko VL, Rutherford WM, Smiley BK, Spielbauer AJ (2006) Ferulate Esterase Producing strains and methods of using same. US patent application 20060046292
Picataggio SK, Zhang M, Franden MA, McMillan JD, Finkelstein M (1998) Recombinant lactobacillus for fermentation of xylose to lactic acid and lactate. US patent 5,798,237
Ranjit NK, Kung L Jr (2000) The effect of Lactobacillus buchneri, Lactobacillus plantarum, or a chemical preservative on the fermentation and aerobic stability of corn silage. J Dairy Sci 83:526–535
Saier MH Jr, Ye JJ, Klinke S, Nino E (1996) Identification of an anaerobically induced phosphoenolpyruvate-dependent fructose-specific phosphotransferase system and evidence for the Embden-Meyerhof glycolytic pathway in the heterofermentative bacterium Lactobacillus brevis. J Bacteriol 178:314–316
Schutz H, Radler F (1984) Anaerobic reduction of glycerol to propanediol-1.3 by Lactobacillus brevis and Lactobacillus buchneri. Syst Appl Microbiol 5:169–78
Skinner KA, Leathers TD (2004) Bacterial contaminants of fuel ethanol production. J Ind Microbiol Biotechnol 31:401–408
Somerville C (2006) The billion-ton biofuels vision. Science 312:1277
Stevenson DM, Muck RE, Shinners KJ, Weimer PJ (2005) Use of real time PCR to determine population profiles of individual species of lactic acid bacteria in alfalfa silage and stored corn stover. Appl Microbiol Biotechnol, pp 1–10
Tanaka K, Komiyama A, Sonomoto K, Ishizaki A, Hall SJ, Stanbury PF (2002) Two different pathways for d-xylose metabolism and the effect of xylose concentration on the yield coefficient of l-lactate in mixed-acid fermentation by the lactic acid bacterium Lactococcus lactis IO-1. Appl Microbiol Biotechnol 60:160–167
Taylor CC, Ranjit NJ, Mills JA, Neylon JM, Kung L Jr (2002) The effect of treating whole-plant barley with Lactobacillus buchneri 40788 on silage fermentation, aerobic stability, and nutritive value for dairy cows. J Dairy Sci 85:1793–1800
Veiga-da-Cunha M, Foster MA (1992) 1,3-Propanediol:NAD+ oxidoreductases of Lactobacillus brevis and Lactobacillus buchneri. Appl Environ Microbiol 58:2005–2010
Veiga da Cunha M, Foster MA (1992) Sugar-glycerol cofermentations in lactobacilli: the fate of lactate. J Bacteriol 174:1013–1019
Weinberg ZG, Muck RE, Weimer PJ (2003) The survival of silage inoculant lactic acid bacteria in rumen fluid. J Appl Microbiol 94:1066–1071
Whitehead TR, Cotta MA (2001) Sequence analyses of a broad host-range plasmid containing ermT from a tylosin-resistant Lactobacillus sp. Isolated from swine feces. Curr Microbiol 43:17–20
Wood BJB, Holzapfel WH (eds) (1995) The genera of lactic acid bacteria, vol 2. Blackie Academic & Professional, London, pp 398
Yildirim M (2001) Characterization of Buchnericin LB Produced by Lactobacillus buchneri LB. Turk J Biol 25:73–82
Yildirim M (2001) Purification of Buchnericin LB Produced by Lactobacillus buchneri LB. Turk J Biol 25:59–65
Yildirim Z, Avsar YK, Yildirim M (2002) Factors affecting the adsorption of buchnericin LB, a bacteriocin produced by Lactobacillus buchneri. Microbiol Res 157:103–107
Acknowledgments
We thank Jacqueline Zane and Melinda S. Nunnally for their excellent technical assistance.
Author information
Authors and Affiliations
Corresponding author
Additional information
Names are necessary to report factually on available data; however, the USDA neither guarantees nor warrants the standard of the product, and the use of the names by USDA implies no approval of the product to the exclusion of others that may also be suitable.
Rights and permissions
About this article
Cite this article
Liu, S., Skinner-Nemec, K.A. & Leathers, T.D. Lactobacillus buchneri strain NRRL B-30929 converts a concentrated mixture of xylose and glucose into ethanol and other products. J Ind Microbiol Biotechnol 35, 75–81 (2008). https://doi.org/10.1007/s10295-007-0267-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10295-007-0267-8