Abstract
Production of gamma-aminobutyric acid (GABA) was carried out in Erlenmeyer flasks by Lactobacillus brevis NCL912. Traditional methods were first adopted to select the key factors that impact the GABA production to preliminarily determine the suitable concentration ranges of the key factors. It was found that glucose, soya peptone, Tween-80 and MnSO4·4H2O were the key factors affecting GABA production. Then, response surface methodology was applied to analyze the optimum contents of the four key factors in the medium, and the production of GABA was predicted as 349.69 mM under the optimized conditions with this model. Afterward, the experiment was performed under the optimized conditions, and the yield of GABA reached 345.83 mM, which was 130% higher than the initial medium. The results showed that experimental yield and predicted values of GABA yield were in good agreement.
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References
Adeghate E, Ponery AS (2002) GABA in the endocrine pancreas: cellular localization and function in normal and diabetic rats. Tissue Cell 34:1–6. doi:10.1054/tice.2001.0217
Cho YR, Chang JY, Chang HC (2007) Production of gamma-aminobutyric acid (GABA) by Lactobacillus buchneri isolated from kimchi and its neuroprotective effect on neuronal cells. J Microbiol Biotechnol 17:104–109
Hagiwara H, Seki T, Ariga T (2004) The effect of pre-germinated brown rice intake on blood glucose and PAI-1 levels in streptozotocin-induced diabetic rats. Biosci Biotechnol Biochem 68:444–447
Hao RY, Schmit JC (1993) Cloning of the gene for glutamate decarboxylase and its expression during conidiation in Neurospora crassa. Biochem J 293:735–738
Huang J et al (2007a) Purification and characterization of glutamate decarboxylase of Lactobacillus brevis CGMCC 1306 isolated from fresh milk. Chin J Chem Eng 15:157–161
Huang J, Mei LH, Xia J (2007b) Application of artificial neural network coupling particle swarm optimization algorithm to biocatalytic production of GABA. Biotechnol Bioeng 96:924–931. doi:10.1002/bit.21162
Jakobs C, Jaeken J, Gibson KM (1993) Inherited disorders of GABA metabolism. J Inherit Metab Dis 16:704–715
Komatsuzaki N et al (2005) Production of gamma-aminobutyric acid (GABA) by Lactobacillus paracasei isolated from traditional fermented foods. Food Microbiol 22:497–504. doi:10.1016/j.fm.2005.01.002
Komatsuzaki N et al (2008) Characterization of glutamate decarboxylase from a high gamma-aminobutyric acid (GABA)-producer, Lactobacillus paracasei. Biosci Biotechnol Biochem 72:278–285. doi:10.1271/bbb.70163
Kono I, Himeno K (2000) Changes in gamma-aminobutyric acid content during beni-koji making. Biosci Biotechnol Biochem 64:617–619
Lee SL, Chen WC (1997) Optimization of medium composition for the production of glucosyltransferase by Aspergillus niger with response surface methodology. Enzyme Microb Tech 21:436–440
Li H et al (2008) A high γ-aminobutyric acid-producing ability: Lactobacillus brevis isolated from Chinese traditional paocai. Ann Microbiol 58:649–653
Li H et al (2009) Pre-staining paper chromatography method for quantification of gamma-aminobutyric acid. J Chromatogr A 1216:5057–5060. doi:10.1016/j.chroma.2009.04.044
Manyam BV et al (1981) Isoniazid-induced elevation of CSF GABA levels and effects on chorea in Huntington’s disease. Ann Neurol 10:35–37
Maras B et al (1992) The amino acid sequence of glutamate decarboxylase from Escherichia coli—evolutionary relationship between mammalian and bacterial enzymes. Eur J Biochem 204:93–98
Mody I et al (1994) Bridging the cleft at gaba synapses in the brain. Trends Neurosci 17:517–525
Nomura M et al (1998) Production of gamma-aminobutyric acid by cheese starters during cheese ripening. J Dairy Sci 81:1486–1491
Park KB, Oh SH (2006) Isolation and characterization of Lactobacillus buchneri strains with high gamma-aminobutyric acid-producing capacity from naturally aged cheese. Food Sci Biotechnol 15:86–90
Park KB, Oh SH (2007) Production of yogurt with enhanced levels of gamma-aminobutyric acid and valuable nutrients using lactic acid bacteria and germinated soybean extract. Bioresour Technol 98:1675–1679. doi:10.1016/j.biortech.2006.06.006
Rastogi NK, Rashmi KR (1999) Optimisation of enzymatic liquefaction of mango pulp by response surface methodology. Eur Food Res Technol 209:57–62
Siragusa S et al (2007) Synthesis of γ-aminobutyric acid by lactic acid bacteria isolated from a variety of Italian cheeses. Appl Environ Microbiol 73:7283–7290. doi:10.1128/AEM.01064-07
Skeie S, Ardo Y (2000) Influence from raw milk flora on cheese ripening studied by different treatments of milk to model cheese. Lebensm-Wiss Technol 33:499–505
Smith DK et al (1992) Escherichia coli has two homologous glutamate decarboxylase genes that map to distinct loci. J Bacteriol 174:5820–5826
Ueno Y et al (1997) Purification and characterization of glutamate decarboxylase from Lactobacillus brevis IFO 12005. Biosci Biotechnol Biochem 61:1168–1171
Wong CG, Bottiglieri T, Snead OC 3rd (2003) GABA, gamma-hydroxybutyric acid, and neurological disease. Ann Neurol 54:S3–S12. doi:10.1002/ana.10696
Yokoyama S, Hiramatsu J, Hayakawa K (2002) Production of gamma-aminobutyric acid from alcohol distillery lees by Lactobacillus brevis IFO-12005. J Biosci Bioeng 93:95–97
Acknowledgments
We thank Dr. Yu Bo for his assistance in the response surface methodology analysis. This work was financially supported by the Education Department of the Jiangxi Province.
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Li, H., Qiu, T., Gao, D. et al. Medium optimization for production of gamma-aminobutyric acid by Lactobacillus brevis NCL912. Amino Acids 38, 1439–1445 (2010). https://doi.org/10.1007/s00726-009-0355-3
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DOI: https://doi.org/10.1007/s00726-009-0355-3