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Wine yeast typing by MALDI-TOF MS

  • Genomics, transcriptomics, proteomics
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Abstract

For the production of wine, the most important industrially used yeast species is Saccharomyces cerevisiae. Years of experience have shown that wine quality and property are significantly affected by the employed strain conducting the fermentation. Consequently, the ability of a strain level differentiation became an important requirement of modern winemaking. In our study, we showed that the differentiation by time-consuming and laborious biochemical and DNA-based methods to enable a constant beverage quality and characteristics can be replaced by matrix-assisted-laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), accompanied by the additional benefit of an application prediction. Mass fingerprints of 33 Saccharomyces strains, which are commonly used for varying wine fermentations, were generated by MALDI-TOF MS upon optimized sample preparation and instrument settings and analyzed by a cluster analysis for strain or ecotype-level differentiation. As a reference method, delta-PCR was chosen to study the genetic diversity of the employed strains. Finally, the cluster analyses of both methods were compared. It could be shown that MALDI-TOF MS, acting at proteome level, provides valuable information about the relationship between yeast strains and their application potential according to their MALDI mass fingerprint.

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Acknowledgements

Part of this work was funded by the German Ministry of Economics and Technology and the Wifö (Wissenschaftsförderung der Deutschen Brauwirtschaft e.V., Berlin, Germany) in project AiF 16576 N. We would like to thank Forbes R. Wardrop and Anne Ortiz-Julien for their assistance in strain selection and critical reading of the manuscript.

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  1. Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Gregor-Mendel Str. 4, 85354, Freising, Germany

    Julia C. Usbeck, Jürgen Behr & Rudi F. Vogel

  2. Biotechnology Research Institute, Lallemand Inc., 6100 Royalmount, Montreal, Québec, H4P 2R2, Canada

    Caroline Wilde & Dave Bertrand

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  1. Julia C. Usbeck
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Correspondence to Jürgen Behr.

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Usbeck, J.C., Wilde, C., Bertrand, D. et al. Wine yeast typing by MALDI-TOF MS. Appl Microbiol Biotechnol 98, 3737–3752 (2014). https://doi.org/10.1007/s00253-014-5586-x

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