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Improved curdlan fermentation process based on optimization of dissolved oxygen combined with pH control and metabolic characterization of Agrobacterium sp. ATCC 31749

  • Applied Microbial and Cell Physiology
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Abstract

A significant problem in scale-down cultures, rarely studied for metabolic characterization and curdlan-producing Agrobacterium sp. ATCC 31749, is the presence of dissolved oxygen (DO) gradients combined with pH control. Constant DO, between 5% and 75%, was maintained during batch fermentations by manipulating the agitation with PID system. Fermentation, metabolic and kinetic characterization studies were conducted in a scale-down system. The curdlan yield, intracellular nucleotide levels and glucose conversion efficiency into curdlan were significantly affected by DO concentrations. The optimum DO concentrations for curdlan production were 45–60%. The average curdlan yield, curdlan productivity and glucose conversion efficiency into curdlan were enhanced by 80%, 66% and 32%, respectively, compared to that at 15% DO. No apparent difference in the gel strength of the resulting curdlan was detected. The comparison of curdlan biosynthesis and cellular nucleotide levels showed that curdlan production had positive relationship with intracellular levels of UTP, ADP, AMP, NAD+, NADH and UDP-glucose. The curdlan productivity under 45% DO and 60% DO was different during 20–50 h. However, after 60 h curdlan productivity of both conditions was similar. On that basis, a simple and reproducible two-stage DO control process for curdlan production was developed. Curdlan production yield reached 42.8 g/l, an increase of 30% compared to that of the single agitation speed control process.

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References

  • Badulescu MM, Apetrei NS, Lupu AR, Cremer L, Szegli G, Moscovici M, Mocanu G, Mihai D, Calugaru A (2009) Curdlan derivatives able to enhance cytostatic drugs activity on tumor cells. Roum Arch Microbiol Immunol 68:201–206

    Google Scholar 

  • Calik P, Yilgor P, Ayhan P, Demir AS (2004) Oxygen transfer effects on recombinant benzaldehyde lyase production. Chem Eng Sci 59:5075–5083. doi:10.1016/j.ces.2004.07.070

    Article  CAS  Google Scholar 

  • Calugaru A, Cremer L, Lupu AR, Badulescu MM, Apetrei NS, Moscovici M, Mocanu G, Mihai D, Kerek F, Szegli G (2009) Recognition and modulation of Dectin-1 and TLR-2 receptors by curdlan derivatives and purified natural extracts. Roum Arch Microbiol Immunol 68:119–124

    CAS  Google Scholar 

  • Campbell MK, Shawn O (2009) Biochemistry, 6th edn. Thomson-Brooks/Cole, Belmont

    Google Scholar 

  • Dennehy KM, Brown GD (2007) The role of the β-glucan receptor Dectin-1 in control of fungal infection. J Leukocyte Biol 82:253–258

    Article  CAS  Google Scholar 

  • Fazenda ML, Harvey LM, McNeil B (2010) Effects of dissolved oxygen on fungal morphology and process rheology during fed-batch processing of Ganoderma lucidum. J Microbiol Biotechnol 20:844–851

    Article  CAS  Google Scholar 

  • Ferwerda G, Meyer-Wentrup F, Kullberg BJ, Netea MG, Adema GJ (2008) Dectin-1 synergizes with TLR2 and TLR4 for cytokine production in human primary monocytes and macrophages. Cell Microbiol 10:2058–2066

    Article  CAS  Google Scholar 

  • Fredlund E, Blank LM, Schnurer J, Sauer U, Passoth V (2004) Oxygen- and glucose-dependent regulation of central carbon metabolism in Pichia anomala. Appl Environ Microb 70:5905–5911. doi:10.1128/AEM.70.10.5905-5911.2004

    Article  CAS  Google Scholar 

  • Fu YQ, Li S, Chen Y, Xu Q, Huang H, Sheng XY (2010) Enhancement of fumaric acid production by Rhizopus oryzae using a two-stage dissolved oxygen control strategy. Appl Biochem Biotechnol 162:1031–1038. doi:10.1007/s12010-009-8831-5

    Article  CAS  Google Scholar 

  • Garcia-Ochoa F, Gomez Castro E, Santos VE (2000) Oxygen transfer and up take rates during xanthan gum production. Enzyme Microb Technol 27:680–690. doi:10.1016/S0141-0229(00)00272-6

    Article  CAS  Google Scholar 

  • Gordon M, Guralnik M, Kaneko Y, Mimura T, Goodgame J, Lang W (1995) Further clinical-studies of curdlan sulfate (Crds): an anti-HIV agent. J Med 26:97–131

    CAS  Google Scholar 

  • Harada T, Kanzawa Y, Kanenaga K, Koreeda A, Harada A (1991) Electron-microscopic studies on the ultrastructure of curdlan and other polysaccharides in gels used in foods. Food Struct 10:1–18

    CAS  Google Scholar 

  • Harwood JE, Huyser DJ (1970) Automated analysis of ammonia in water. Water Res 4:695–704

    Article  Google Scholar 

  • Jagodzinski PP, Wiaderkiewicz R, Kurzawski G, Kloczewiak M, Nakashima H, Hyjek E, Yamamoto N, Uryu T, Kaneko Y, Posner MR, Kozbor D (1994) Mechanism of the inhibitory effect of curdlan sulfate on HIV-1 infection in vitro. Virology 202:735–745. doi:10.1006/viro.1994.1395

    Article  CAS  Google Scholar 

  • Jin LH, Um HJ, Yin CJ, Kim YH, Lee JH (2008) Proteomic analysis of curdlan-producing Agrobacterium sp in response to pH downshift. J Biotechnol 138:80–87. doi:10.1016/j.jbiotec.2008.08.010

    Article  CAS  Google Scholar 

  • Kataoka K, Muta T, Yamazaki S, Takeshige K (2002) Activation of macrophages by linear (1 → 3)-β-d-glucans implications for the recognition of fungi by innate immunity. J Biol Chem 277:36825–36831. doi:10.1074/jbc.M206756200

    Article  CAS  Google Scholar 

  • Kaya-Celiker H, Angardi V, Calik P (2009) Regulatory effects of oxygen transfer on overexpression of recombinant benzaldehyde lyase production by Escherichia coli BL21 (DE3). Biotechnol J 4:1066–1076. doi:10.1002/biot.200800255

    Article  CAS  Google Scholar 

  • Kim MK, Lee IY, Ko JH, Rhee YH, Park YH (1999) Higher intracellular levels of uridinemonophosphate under nitrogen-limited conditions enhance metabolic flux of curdlan synthesis in Agrobacterium species. Biotechnol Bioeng 62:317–323. doi:10.1002/(SICI)1097-0290(19990205

    Article  CAS  Google Scholar 

  • Kim BS, Jung ID, Kim JS, Lee J, Lee IY, Lee KB (2000a) Curdlan gels as protein drug delivery vehicles. Biotechnol Lett 22:1127–1130. doi:10.1023/A:1005636205036

    Article  CAS  Google Scholar 

  • Kim MK, Lee IY, Lee JH, Kim KT, Rhee YH, Park YH (2000b) Residual phosphate concentration under nitrogen-limiting conditions regulates curdlan production in Agrobacterium species. J Ind Microbiol Biotechnol 25:180–183. doi:10.1038/sj.jim.7000053

    Article  CAS  Google Scholar 

  • Kim MK, Ryu KE, Choi WA, Rhee YH, Lee IY (2003) Enhanced production of (1 → 3)-β-d-glucan by a mutant strain of Agrobacterium species. Biochem Eng J 16:163–168

    Article  CAS  Google Scholar 

  • Kim HH, Na JG, Chang YK, Lee SJ (2005) Effects of dissolved oxygen control on cell growth and exopolysaccharides production in batch culture of Agaricus blazei. Korean J Chem Eng 22(1):80–84

    Article  CAS  Google Scholar 

  • Kumar H, Kumagai Y, Tsuchida T, Koenig PA, Satoh T, Guo ZJ, Jang MH, Saitoh T, Akira S, Kawai T (2009) Involvement of the NLRP3 inflammasome in innate and humoral adaptive immune responses to fungal β-glucan. J Immunol 183:8061–8067. doi:10.4049/jimmunol.0902477

    Article  CAS  Google Scholar 

  • Lawford HG, Rousseau JD (1992) Production of β-1,3-glucan exopolysaccharide in low shear systems the requirement for high oxygen-tension. Appl Biochem Biotechnol 34:597–612. doi:10.1007/BF02920581

    Article  Google Scholar 

  • Lawford H, Keenan J, Phillips K, Orts W (1986) Influence of bioreactor design on the rate and amount of curdlan-type exopolysaccharide production by Alcaligenes faecalis. Biotechnol Lett 8:145–150. doi:10.1007/BF01029368

    Article  CAS  Google Scholar 

  • Lee JH, Lee IY (2001) Optimization of uracil addition for curdlan (β-1 → 3-glucan) production by Agrobacterium sp. Biotechnol Lett 23:1131–1134. doi:10.1023/A:1010516001444

    Article  CAS  Google Scholar 

  • Lee JH, Park YH (2001) Optimal production of curdlan by Agrobacterium sp with feedback inferential control of optimal pH profile. Biotechnol Lett 23:525–530. doi:10.1023/A:1010374519891

    Article  CAS  Google Scholar 

  • Lee IY, Kim MK, Lee JH, Seo WT, Jung JK, Lee HW, Park YH (1999a) Influence of agitation speed on production of curdlan by Agrobacterium species. Bioproc Eng 20:283–287. doi:10.1007/PL00009049

    CAS  Google Scholar 

  • Lee JH, Lee IY, Kim MK, Park YH (1999b) Optimal pH control of batch processes for production of curdlan by Agrobacterium species. J Ind Microbiol Biotechnol 23:143–148. doi:10.1038/sj.jim.2900714

    Article  CAS  Google Scholar 

  • Levander F, Svensson M, Radstrom P (2002) Enhanced exopolysaccharide production by metabolic engineering of Streptococcus thermophilus. Appl Environ Microb 68:784–790. doi:10.1128/AEM.68.2.784-790.2002

    Article  CAS  Google Scholar 

  • Liu YS, Wu HY, Ho KP (2006) Characterization of oxygen transfer conditions and their effects on Phaffia rhodozyma growth and carotenoid production in shake-flask cultures. Biochem Engin 27:331–335. doi:10.1016/j.bej.2005.08.031

    Article  Google Scholar 

  • Martin AB, Alcon A, Santos VE, Garcia-Ochoa F (2005) Production of a biocatalyst of Pseudomonas putida CECT5279 for DBT biodesulfurization: influence of the operational conditions. Energy Fuel 19:775–782. doi:10.1021/ef0400417

    Article  CAS  Google Scholar 

  • Moscovici M, Ionescu C, Oniscu C, Fotea O, Protopopescu P, Hanganu D (1996) Improved exopolysaccharide production in fed-batch fermentation of Aureobasidium pullulans, with increased impeller speed. Biotechnol Lett 18:787–790. doi:10.1007/BF00127889

    Article  CAS  Google Scholar 

  • Nakao Y (1991) Curdlan: properties and application to foods. J Jpn Soc Food Sci Technol 38:736–742

    Article  Google Scholar 

  • Puliga SL, Handa S, Gummadi SN, Doble M (2010) Enhancement and scale-up of β-(1 → 3) glucan production by Agrobacterium sp. Int J Food Eng. doi:10.2202/1556-3758.1736

  • Sandoval-Basurto EA, Gosset G, Bolivar F, Ramirez OT (2005) Culture of Escherichia coli under dissolved oxygen gradients simulated in a two-compartment scale-down system: metabolic response and production of recombinant protein. Biotechnol Bioeng 89:453–463. doi:10.1002/bit.20383

    Article  CAS  Google Scholar 

  • Wang Y, McNeil B (1995) Dissolved oxygen and the scleroglucan fermentation process. Biotechnol Lett 17:257–17262. doi:10.1007/BF01190633

    Article  CAS  Google Scholar 

  • Wu J, Zhan X, Liu H, Zheng Z (2008) Enhanced production of curdlan by Alcaligenes faecalis by selective feeding with ammonia water during the cell growth phase of fermentation. Chin J Biotechnol 24:1035–1039

    Article  CAS  Google Scholar 

  • Yu LJ, Wu JR, Liu J, Zhan XB, Zheng ZY, Lin CC (2011) Enhanced curdlan production in Agrobacterium sp. ATCC 31749 by addition of low-polyphosphates. Biotechnol. Bioprocess Eng 16:34–41. doi:10.1007/s12257-010-0145-5

    Article  CAS  Google Scholar 

  • Zhang H-T, Zhan X-B, Zheng Z-Y, Wu J-R, Yu X-B, Jiang Y, Lin CC (2011) Sequence and transcriptional analysis of the genes responsible for curdlan biosynthesis in Agrobacterium sp. ATCC 31749 under simulated dissolved oxygen gradients conditions. Appl Microbiol Biotechnol. doi:10.1007/s00253-011-3243-1

  • Zheng ZY, Lee JW, Zhan XB, Shi Z, Wang L, Zhu L, Wu JR, Lin CC (2007) Effect of metabolic structures and energy requirements on curdlan production by Alcaligenes faecalis. Biotechnol Bioprocess Eng 12:359–365. doi:10.1007/BF02931057

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by research grants from the National Natural Science Foundation of China (20676055, 60604028 and 20806034), Ministry of Science and Technology of China (National Basic Research Program of China, 2007CB714303) and Research on the Preparation Technology of Edible Emulsifier and its Industrialization (National Key-technologies R&D Program of China, 2011BAD23B00). The work was also supported by the Program for Introducing Talents of Discipline to the Universities, No. 111-2-06. The authors would like to thank these organizations for their kind financial support.

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Authors and Affiliations

  1. Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Lihu Road 1800, Wuxi, 214122, China

    Hong-Tao Zhang, Xiao-Bei Zhan, Zhi-Yong Zheng, Jian-Rong Wu, Xiao-Bin Yu & Chi-Chung Lin

  2. Key Laboratory of Glycochemistry and Glycobiotechnology of Ministry of Education, Jiangnan University, Lihu Road 1800, Wuxi, 214122, China

    Xiao-Bei Zhan

  3. Faculty of Medicine, Imperial College London, Northwick Park campus, London, HA1 3GX, UK

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  1. Hong-Tao Zhang
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Correspondence to Xiao-Bei Zhan.

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Zhang, HT., Zhan, XB., Zheng, ZY. et al. Improved curdlan fermentation process based on optimization of dissolved oxygen combined with pH control and metabolic characterization of Agrobacterium sp. ATCC 31749. Appl Microbiol Biotechnol 93, 367–379 (2012). https://doi.org/10.1007/s00253-011-3448-3

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  • DOI: https://doi.org/10.1007/s00253-011-3448-3

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