Skip to main content
SpringerLink
Log in

Dissolved oxygen and the scleroglucan fermentation process

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

The effect of culture dissolved oxygen (D.O.) upon biomass, scleroglucan and oxalate formation bySclerotium glucanicum was examined in a stirred tank fermenter by oxygen enrichment. Controlling culture D.O. at 5 or 10% saturation led to increased biomass formation and decreased scleroglucan formation. The mechanism by which this occurred probably involved a non-specific diversion of C source (sucrose) away from product and towards biomass synthesis. This is at variance with the reported “stimulatory” effect of limiting D.O. levels upon scleroglucan synthesis. Controlling the culture at higher levels (20 and 30%) involved increases in impeller speed. The results of such changes were distinct from those of D.O., thus demonstrating that attempts to examine D.O. effects by means of impeller speed changes are inappropriate.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Brigand, B. (1993). In:Industrial Gums, Academic Press, New York. pp. 461–474.

    Google Scholar 

  2. McNeil, B. and Harvey, L.M. (1993).Crit. Rev. Biotechnol. 13(4), 275–304.

    Google Scholar 

  3. Pretus, H.A., Ensley, H.E., McNamee, R.B., Jones, E.L., Browder, I.W. and Williams, P.L. (1990).J. Pharmacol. Exp, Therap. 257, 500–510.

    Google Scholar 

  4. Holzworth, G. (1985).Dev. Ind. Microbiol.,26, 271–280.

    Google Scholar 

  5. Wang, Y. and McNeil, B. (1994).Biotechnol. Letts. 16, 605–610.

    Google Scholar 

  6. Rau, V., Gura, E., Olzewski, E. and Wagner, F. (1992).J. Ind. Microbiol. 9, 19–26.

    Google Scholar 

  7. Rho, D., Mulchandani, A., Luong, J.H.T. and LeDuy, A. (1988).Appl. Microbiol. Technol.,28, 361–366.

    Google Scholar 

  8. Rau, V., Miller, R-J., Cordes, K. and Klein, J. (1990).Bioprocess Eng. 5, 89–93.

    Google Scholar 

  9. Wang, Y. and McNeil, B. (in press).Enz. Microbiol. Technol.

  10. Wernau, W.C. (1985).Dev. Ind. Microbiol. 26(4), 263–269.

    Google Scholar 

  11. Dubois, M., Gilles, K.A., Hamilton, J.K., Revers, P.A. and Smith, F. (1956).Analyt. Chem. 28, 350–357.

    Google Scholar 

  12. Allan, D.G. and Robinson, C.W. (1989).Biotechnol. Bioeng. 34, 731–740.

    Google Scholar 

  13. Onken, V. and Liefke, E. (1989).Adv. Biochem. Eng. 40, 137–169.

    Google Scholar 

  14. Pilz, F., Auling, E., Stephan, D., Rau, V. and Wagner, F. (1991).Exp. Mycol. 15, 181–192.

    Google Scholar 

  15. Maxwell, D.P. and Bateman, D.F. (1968).Phytopathol. 58, 1635–1642.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Bioscience and Biotechnology, University of Strathclyde, 204 George Street, G1 1XW, Glasgow, UK

    Y. Wang & B McNeil

Authors
  1. Y. Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B McNeil
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, Y., McNeil, B. Dissolved oxygen and the scleroglucan fermentation process. Biotechnol Lett 17, 257–262 (1995). https://doi.org/10.1007/BF01190633

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01190633

Keywords

Search

Navigation