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Effect of metabolic structures and energy requirements on curdlan production byAlcaligenes faecalis

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Abstract

A comprehensive metabolic network was proposed forAlcaligenes faecalis and employed in a stoichiometrically based flux balance model for curdlan production optimization. The maximal yield of curdlan was evaluated for curdlan batch production. Various metabolic structures and metabolic pathway distributions related with the curdlan maximal yield was evaluated. The results showed that the energy efficiency rather than the substrate supply was the major constraint for the enhancement of curdlan production. The increase in specific rate of glucose uptake could enhance curdlan production yield due to the decrease of the ratio of metabolic maintenance to substrate consumption. However, some of the energy loss and nutrient limitation associated with the increase of metabolic maintenance would adversely affect the conversion efficiency of the substrate.

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Abbreviations

6-P-G:

6-Phosphogluconate

AcCoA:

Acetyl coenzyme A

DHAP:

Dihydroxyacetone-phosphate

DCW:

Dry cell weight

F-6-P:

Fructose-6-phosphate

FDP:

Fructose-1,6-bisphosphate

G-1-P:

Glucose-1-phosphate

G-6-P:

Glucose-6-phosphate

GAP:

Glyceraldehyde-3-phosphate

KDPG:

2-Keto-3-deoxy-6-phosphogluconate

PEP:

Phosphoenolpyruvate

PQQ:

Pyrroloquinoline quinine

PQQH2 :

Dihydroquinone PQQ

PYR:

Pyruvate

Ri-5-P:

Ribose-5-phosphate

TCA:

Tricarboxylic acid cycle

UDPG:

UDP-glucose

Lipid-P:

Isoprenoid lipid-phosphate

Lipid-P-G:

Isoprenoid lipid-phosphoglucose

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Authors and Affiliations

  1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, 214122, Wuxi, P. R. China

    Zhi-Yong Zheng, Jin Woo Lee, Xiao Bei Zhan, Zhongping Shi, Lei Wang, Li Zhu, Jian-Rong Wu & Chi Chung Lin

  2. Faculty of Natural Resources and Life Science, Dong-A University, 604-714, Pusan, Korea

    Jin Woo Lee

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  1. Zhi-Yong Zheng
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  2. Jin Woo Lee
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Correspondence to Xiao Bei Zhan.

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Zheng, ZY., Lee, J.W., Zhan, X.B. et al. Effect of metabolic structures and energy requirements on curdlan production byAlcaligenes faecalis . Biotechnol. Bioprocess Eng. 12, 359–365 (2007). https://doi.org/10.1007/BF02931057

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  • DOI: https://doi.org/10.1007/BF02931057

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