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Engineering of nitrogen metabolism and its regulation in Corynebacterium glutamicum: influence on amino acid pools and production

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Abstract

Nitrogen is one of the macronutrients necessary for living cells, and consequently, assimilation of nitrogen is a crucial step for metabolism. To satisfy their nitrogen demand and to ensure a sufficient nitrogen supply even in situations of nitrogen limitation, microorganisms have evolved sophisticated uptake and assimilation mechanisms for different nitrogen sources. This mini-review focuses on nitrogen metabolism and its control in the biotechnology workhorse Corynebacterium glutamicum, which is used for the industrial production of more than 2 million tons of l-amino acids annually. Ammonium assimilation and connected control mechanisms on activity and transcription level are summarized, and the influence of mutations on amino acid pools and production is described with emphasis on l-glutamate, l-glutamine, and l-lysine.

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Acknowledgments

Work of the authors’ related to nitrogen metabolism and control was supported by the Deutsche Forschungsgemeinschaft (SFB473, SFB635) and the Bundesministerium für Bildung und Forschung (in frame of the programs GenoMik, GenoMik+, GenoMik-Transfer and SysMAP). The help of A. Reth (Evonik) with patent searches is gratefully aknowledged.

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  1. Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, 91058, Erlangen, Germany

    Nadine Rehm & Andreas Burkovski

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Rehm, N., Burkovski, A. Engineering of nitrogen metabolism and its regulation in Corynebacterium glutamicum: influence on amino acid pools and production. Appl Microbiol Biotechnol 89, 239–248 (2011). https://doi.org/10.1007/s00253-010-2922-7

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