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Colorimetric sensor based on peroxidase-like activity of chitosan coated on magnetic nanoparticles for rapid detection of the total bacterial count in raw milk

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Abstract

Recently, frequent outbreaks of foodborne diseases have attracted increasing attention, and how to rapidly detect foodborne pathogens has also become an urgent issue. Herein, we reported the colorimetric sensor based on chitosan-coated magnetic nanoparticles (CS@MNPs) for rapidly broad-spectrum detection of the total bacterial count, whose color change can be visible to the naked eye without any other sophisticated instruments. CS@MNPs exhibited good capture ability (greater than 80%) for six model strains, including Escherichia coli O157:H7, Salmonella enterica serovar typhimurium, Staphylococcus aureus, Listeria monocytogenes, Bacillus cereus and Cronobacter sakazakii, due to their interaction with chitosan. Under optimized conditions, the colorimetric sensor showed a linear relationship of mixed bacteria within the concentration ranging from 2.30 × 101 to 2.30 × 107 CFU·mL−1 in pure culture, and 8.50 × 101 to 8.50 × 106 CFU·mL−1 in spiked raw milk within 15 min. Besides, compared with the traditional one, colorimetric method is a more rapid and efficient method that can be applied to the dairy industry to detect the total bacterial count. In our perception, the colorimetric sensor developed in this work provides a possibility for on-set detection of the total bacterial count in other research fields.

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Funding

This study was funded by the National Natural Science Foundation of China [No. 32172320] and the Natural Science Foundation of Guangdong Province [No. 2021A1515011068].

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Authors and Affiliations

  1. School of Food Science and Engineering, South China University of Technology, Guangzhou City, 510640, Guangdong Province, China

    Luying Wang, Hong Bai, Ximin Liu, Xinglong Xiao, Yigang Yu & Xiaofeng Li

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  1. Luying Wang
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Correspondence to Xinglong Xiao or Xiaofeng Li.

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Luying Wang declares no conflict of interest. Hong Bai declares no conflict of interest. Ximin Liu declares no conflict of interest. Xinglong Xiao declares no conflict of interest. Yigang Yu declares no conflict of interest. Xiaofeng Li declares no conflict of interest.

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Wang, L., Bai, H., Liu, X. et al. Colorimetric sensor based on peroxidase-like activity of chitosan coated on magnetic nanoparticles for rapid detection of the total bacterial count in raw milk. Eur Food Res Technol 248, 1321–1333 (2022). https://doi.org/10.1007/s00217-022-03970-8

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  • DOI: https://doi.org/10.1007/s00217-022-03970-8

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