Abstract
Staphylococcus aureus (S. aureus) is an important foodborne pathogen that causes food poisoning and severe infections. Herein, we design a novel and sensitive colorimetric immunoassay for the detection of S. aureus based on the formation of a magnetic bead-based sandwich complex and signal amplification via an enzyme mimic. Magnetic nanoparticles decorated with carbon dots offer rapid enrichment and separation of target bacteria in complex matrices. Sliver nanoclusters are used as an artificial enzyme that can oxidize o-phenylenediamine to form a yellow product with a maximum absorption peak at 421 nm. This method can visually detect S. aureus ranging from 10 to 106 cfu mL−1. Its limit of detection reaches down to 4.9 cfu mL−1. By analyzing food samples, the recovery is from 85.6 to 103.7%.
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References
Bhaisare ML, Gedda G, Khan MS, Wu HF (2016) Fluorimetric detection of pathogenic bacteria using magnetic carbon dots. Anal Chim Acta 920:63–71. https://doi.org/10.1016/j.aca.2016.02.025
Cho IH, Mauer L, Irudayaraj J (2014) In-situ fluorescent immunomagnetic multiplex detection of foodborne pathogens in very low numbers. Biosens Bioelectron 57:143–148. https://doi.org/10.1016/j.bios.2014.02.012
Ding YN, Yang BC, Liu H, Liu ZX, Zhang X, Zheng XW, Liu QY (2018) FePt-Au ternary metallic nanoparticles with the enhanced peroxidase-like activity for ultrafast colorimetric detection of H2O2. Sensors and Actuators B-Chemical 259:775–783. https://doi.org/10.1016/j.snb.2017.12.115
Gao L, Giglio KM, Nelson JL, Sondermann H, Travis AJ (2014) Ferromagnetic nanoparticles with peroxidase-like activity enhance the cleavage of biological macromolecules for biofilm elimination. Nanoscale 6(5):2588–2593. https://doi.org/10.1039/c3nr05422e
Gao ZQ, Xu MD, Hou L, Chen GN, Tang DP (2013) Irregular-shaped platinum nanoparticles as peroxidase mimics for highly efficient colorimetric immunoassay. Anal Chim Acta 776:79–86. https://doi.org/10.1016/j.aca.2013.03.034
Gonzalez-Salamo J, Socas-Rodriguez B, Hernandez-Borges J, Rodriguez-Delgado MA (2016) Nanomaterials as sorbents for food sample analysis. Trac-Trends in Analytical Chemistry 85:203–220. https://doi.org/10.1016/j.trac.2016.09.009
Le Guevel X, Hotzer B, Jung G, Hollemeyer K, Trouillet V, Schneider M (2011) Formation of fluorescent metal (Au, Ag) nanoclusters capped in bovine serum albumin followed by fluorescence and spectroscopy. J Phys Chem C 115(22):10955–10963. https://doi.org/10.1021/jp111820b
Leonard, P., Hearty, S., Brennan, J., Dunne, L., Quinn, J., Chakraborty, T., & O'Kennedy, R. (2003). Advances in biosensors for detection of pathogens in food and water. Enzyme and Microbial Technology, 32(1), 3-13. doi:Pii S0141-0229(02)00232-6
Liu QY, Yang YT, Li H, Zhu RR, Shao Q, Yang SG, Xu JJ (2015) NiO nanoparticles modified with 5,10,15,20-tetrakis(4-carboxyl pheyl)-porphyrin: promising peroxidase mimetics for H2O2 and glucose detection. Biosens Bioelectron 64:147–153. https://doi.org/10.1016/j.bios.2014.08.062
Liu YS, Wang J, Song XL, Xu K, Chen HS, Zhao C, Li J (2018) Colorimetric immunoassay for Listeria monocytogenes by using core gold nanoparticles, silver nanoclusters as oxidase mimetics, and aptamer-conjugated magnetic nanoparticles. Microchim Acta 185(8):1–7. https://doi.org/10.1007/s00604-018-2896-1
Liu YS, Zhao C, Song XL, Xu K, Wang J, Li J (2017) Colorimetric immunoassay for rapid detection of Vibrio parahaemolyticus. Microchim Acta 184(12):4785–4792. https://doi.org/10.1007/s00604-017-2523-6
Niemz A, Ferguson TM, Boyle DS (2011) Point-of-care nucleic acid testing for infectious diseases. Trends Biotechnol 29(5):240–250. https://doi.org/10.1016/j.tibtech.2011.01.007
Pang B, Ding X, Wang GP, Zhao C, Xu YA, Fu KY et al (2017) Rapid and quantitative detection of Vibrio parahemolyticus by the mixed-dye-based loop-mediated isothermal amplification assay on a self-priming compartmentalization microfluidic chip. J Agric Food Chem 65(51):11312–11319. https://doi.org/10.1021/acs.jafc.7b03655
Pang B, Zheng Y, Wang J, Liu Y, Song X, Li J, Yao S, Fu K, Xu K, Zhao C, Li J (2019) Colorimetric detection of Staphylococcus aureus using gold nanorods labeled with yolk immunoglobulin and urease, magnetic beads, and a phenolphthalein impregnated test paper. Mikrochim Acta 186(9):611. https://doi.org/10.1007/s00604-019-3722-0
Park B, Choi SJ (2017) Sensitive immunoassay-based detection of Vibrio parahaemolyticus using capture and labeling particles in a stationary liquid phase lab-on-a-chip. Biosens Bioelectron 90:269–275. https://doi.org/10.1016/j.bios.2016.11.071
Rubab M, Shahbaz HM, Olaimat AN, Oh DH (2018) Biosensors for rapid and sensitive detection of Staphylococcus aureus in food. Biosens Bioelectron 105:49–57. https://doi.org/10.1016/j.bios.2018.01.023
Shukla S, Lee G, Song X, Park S, Kim M (2016) Immunoliposome-based immunomagnetic concentration and separation assay for rapid detection of Cronobacter sakazakii. Biosens Bioelectron 77:986–994. https://doi.org/10.1016/j.bios.2015.10.077
Trncikova T, Hruskova V, Oravcova K, Pangallo D, Kaclikova E (2009) Rapid and sensitive detection of Staphylococcus aureus in food using selective enrichment and real-time PCR targeting a new gene marker. Food Anal Methods 2(4):241–250. https://doi.org/10.1007/s12161-008-9056-x
Wu J, Wei X, Gan JR, Huang L, Shen T, Lou JT et al (2016) Multifunctional magnetic particles for combined circulating tumor cells isolation and cellular metabolism detection. Adv Funct Mater 26(22):4016–4025. https://doi.org/10.1002/adfm.201504184
Yang X, Wang EK (2011) A nanoparticle autocatalytic sensor for Ag+ and Cu2+ ions in aqueous solution with high sensitivity and selectivity and its application in test paper. Anal Chem 83(12):5005–5011. https://doi.org/10.1021/ac2008465
Yuan X, Tay YQ, Dou XY, Luo ZT, Leong DT, Xie JP (2013) Glutathione-protected silver nanoclusters as cysteine-selective fluorometric and colorimetric probe. Anal Chem 85(3):1913–1919. https://doi.org/10.1021/ac3033678
Zhang ZH, Xiao LL, Lou Y, Jin MT, Liao C, Malakar PK et al (2015) Development of a multiplex real-time PCR method for simultaneous detection of Vibrio parahaemolyticus, listeria monocytogenes and Salmonella spp. in raw shrimp. Food Control 51:31–36. https://doi.org/10.1016/j.foodcont.2014.11.007
Zhu XX, Chen W, Wu KL, Li HY, Fu M, Liu QY, Zhang X (2018) A colorimetric sensor of H2O2 based on Co3O4-montmorillonite nanocomposites with peroxidase activity. New J Chem 42(2):1501–1509. https://doi.org/10.1039/c7nj03880a
Funding
This work is funded by National Natural Science Foundation of China (Grant No. 81602895 and 81,602,894), China Postdoctoral Science Foundation (Grant No. 2017T100214 and 2016M591492), Department of Science and Technology of Jilin Province (Grant No. 20160520167JH, 20170204003SF, and 20180520132JH), and Education Department of Jilin Province (Grant No. JJKH20170873KJ and JJKH20180240KJ).
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Shuo YAO declares that she has no conflict of interest. Chao ZHAO declares that he has no conflict of interest. Yushen LIU declares that she has no conflict of interest. Heran NIE declares that he has no conflict of interest. Gaolei XI declares that he has no conflict of interest. Xiaolian CAO declares that she has no conflict of interest. Zhuolin LI declares that she has no conflict of interest. Bo PANG declares that he has no conflict of interest. Juan LI declares that she has no conflict of interest. Juan WANG declares that she has no conflict of interest.
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Yao, S., Zhao, C., Liu, Y. et al. Colorimetric Immunoassay for the Detection of Staphylococcus aureus by Using Magnetic Carbon Dots and Sliver Nanoclusters as o-Phenylenediamine-Oxidase Mimetics. Food Anal. Methods 13, 833–838 (2020). https://doi.org/10.1007/s12161-019-01683-5
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DOI: https://doi.org/10.1007/s12161-019-01683-5