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β-Ionone arrests cell cycle of gastric carcinoma cancer cells by a MAPK pathway

  • Molecular Toxicology
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Abstract

β-Ionone is an end ring analog of β-carotenoid which has been shown to possess potent anti-proliferative activity both in vitro and in vivo. To investigate the possible inhibitory effects of β-ionone, we studied cell growth characteristics, DNA synthesis, cell cycle progression, as well as mitogen-activated protein kinases (MAPKs) pathways in the human gastric adenocarcinoma cancer cell line (SGC-7901). Our results show that cell growth and DNA synthesis were inhibited, and the cell cycle was arrested at the G0/G1 phase in a dose-dependent manner in cells treated with β-ionone (25, 50, 100 and 200 μmol/L) for 24 h. We found that the β-ionone significantly decreased the extracellular signal-regulated kinase protein expression and significantly increased the levels of p38 and Jun-amino-terminal kinase protein expression (P < 0.01). β-Ionone also inhibited cell cycle-related proteins of Cdk4, Cyclin B1, D1 and increased p27 protein expression in SGC-7901 cells. These results suggested that the cell cycle arrest observed may be regulated through a MAPK pathway by transcriptional down-regulation of cell cycle proteins. These results demonstrate potent ability of β-ionone to arrest cell cycle of SGC-7901 cells and decrease proliferation.

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Acknowledgments

This research was supported by the National Natural Science Fund (No. 30200229 and 30772511), HeiLongJiang Postdoctoral Fund (No. LRB03-000120), planning program of key problems from Sanitation Bureau of Heilongjiang province (No. 2007-470), and Department of Education, HeiLongJiang Province (No. 12521274), People’s Republic of China. Hong-Wei Dong, Shuang Zhang and Wen-Guang Sun are co-first authors. Jia-Ren Liu and Ming-Song Li are co-corresponding authors. There are no conflicts of interest in this study.

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Authors and Affiliations

  1. Harbin Medical University, 157 BaoJian Road, NanGang District, Harbin, 150081, People’s Republic of China

    Hong-Wei Dong, Wen-Guang Sun, Lian-Xin Liu & Jia-Ren Liu

  2. Internal Medicine, The Second Affiliated Hospital, Harbin Medical University, 246 XueFu Road, NanGang District, Harbin, 150081, People’s Republic of China

    Shuang Zhang

  3. Department of Pediatric Surgery, GanNan Medical University, 1 YiXueYuan Road, GanZhou, 341000, Jiangxi, People’s Republic of China

    Qian Liu

  4. Boston Children’s Hospital, Harvard Medical School, 300 Longwood AVE, Boston, MA, 02115-5737, USA

    Juan C. Ibla, Sulpicio G. Soriano, Xiao-Hui Han & Jia-Ren Liu

  5. Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, People’s Republic of China

    Ming-Song Li

  6. Guangdong Provincial Key Laboratory of Gastroenterology, Guangzhou, 510515, People’s Republic of China

    Ming-Song Li

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  1. Hong-Wei Dong
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  8. Lian-Xin Liu
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Correspondence to Ming-Song Li or Jia-Ren Liu.

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Hong-Wei Dong, Shuang Zhang, Wen-Guang Sun and Qian Liu are co-first authors.

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Dong, HW., Zhang, S., Sun, WG. et al. β-Ionone arrests cell cycle of gastric carcinoma cancer cells by a MAPK pathway. Arch Toxicol 87, 1797–1808 (2013). https://doi.org/10.1007/s00204-013-1041-5

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  • DOI: https://doi.org/10.1007/s00204-013-1041-5

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