Abstract
Aspergillus sp. A31 and Curvularia geniculata P1 are endophytes that colonize the roots of Aeschynomene fluminensis Vell. and Polygonum acuminatum Kunth. in humid environments contaminated with mercury. The two strains mitigated mercury toxicity and promoted Oryza sativa L growth. C. geniculata P1 stood out for increasing the host biomass by fourfold and reducing the negative effects of the metal on photosynthesis. Assembling and annotation of Aspergillus sp. A31 and C. geniculata P1 genomes resulted in 28.60 Mb (CG% 53.1; 10,312 coding DNA sequences) and 32.92 Mb (CG% 50.72; 8,692 coding DNA sequences), respectively. Twelve and 27 genomes of Curvularia/Bipolaris and Aspergillus were selected for phylogenomic analyzes, respectively. Phylogenetic analysis inferred the separation of species from the genus Curvularia and Bipolaris into different clades, and the separation of species from the genus Aspergillus into three clades; the species were distinguished by occupied niche. The genomes had essential gene clusters for the adaptation of microorganisms to high metal concentrations, such as proteins of the phytoquelatin–metal complex (GO: 0090423), metal ion binders (GO: 0046872), ABC transporters (GO: 0042626), ATPase transporters (GO: 0016887), and genes related to response to reactive oxygen species (GO: 0000302) and oxidative stress (GO: 0006979). The results reported here help to understand the unique regulatory mechanisms of mercury tolerance and plant development.
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Acknowledgements
The research was supported by resources provided by The Mato Grosso State Research Foundation (FAPEMAT), National Council for Scientific and Technological Development (CNPq), Coordination for the Improvement of Higher Education Personnel (CAPES), and the National Institute of Science and Technology in Wet Areas (INAU). This study was carried out with the assistance of the UFV Cluster from Federal University of Viçosa (Viçosa, MG, Brazil).
Funding
Fundação de Amparo à Pesquisa do Estado de Mato Grosso (FAPEMAT, grant 568258/2014), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, grant 409062/2018–9).
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Growth promotion assay [William Pietro-Souza]; assembly of genomes [Tiago Antônio de Oliveira Mendes]; annotation, analysis of genomes, writing and correction of the manuscript [Kátia Aparecida de Siqueira]; data analysis and writing of the manuscript [Jaqueline Alves Senabio]; analysis of CAZymes, writing, correction and revision of the manuscript [Marcos Antônio Soares].
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de Siqueira, K.A., Senabio, J.A., Pietro-Souza, W. et al. Aspergillus sp. A31 and Curvularia geniculata P1 mitigate mercury toxicity to Oryza sativa L. Arch Microbiol 203, 5345–5361 (2021). https://doi.org/10.1007/s00203-021-02481-6
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DOI: https://doi.org/10.1007/s00203-021-02481-6