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SPL5, a cell death and defense-related gene, encodes a putative splicing factor 3b subunit 3 (SF3b3) in rice

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Abstract

A lesion-mimic phenotype in rice (Oryza sativa L.) spotted leaf 5 (spl5) indicates that wild-type SPL5 negatively regulates cell death and resistance responses. Previously, the spl5 gene was already mapped to the 80-kb region between two markers SSR7 and RM7121 through a map-based cloning approach. Here, we further showed that the spl5 gene was delimitated into a 15.1-kb genomic region by the high-resolution sequence target site (STS) markers. Subsequent sequencing in this region of spl5 mutant revealed that one candidate gene harbored a single-base deletion, resulting in a frame-shift mutation and a premature stop codon. Bioinformatic analysis showed that SPL5 gene encodes a putative splicing factor 3b subunit 3 (SF3b3) and might be involved in splicing reactions of pre-mature RNAs participating in the regulation of cell death and resistance responses. Further analysis showed that wild-type SPL5 did functionally complement the spl5 phenotype. The data presented here clearly indicate that the SPL5 negatively regulates cell death and resistance responses via modulating RNA splicing in plants.

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Acknowledgments

This work was supported by the National Key Programs for Transgenic Crops (2011ZX08009-003, 001; 2009ZX08009-042B), the National Natural Science Foundation of China (31171519, 31101130); the Natural Science Foundation of Zhejiang Province (Y3110234, Y3100531), Zhejiang Province Key Innovative Research Team Program (2010R50024), the Welfare Technology Application Research Project of Zhejiang Province (2011C22005), and the Open Project of State Key Laboratory of Rice Biology of China National Rice Research Institute.

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  1. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, 321004, China

    Xifeng Chen, Liang Hao, Jianwei Pan, Xixi Zheng, Yang Jin, Zhimin Gu & Bojun Ma

  2. Institute of Genetic and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

    Xifeng Chen, Guanghuai Jiang & Wenxue Zhai

  3. China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 310006, China

    Qian Qian

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  1. Xifeng Chen
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Correspondence to Wenxue Zhai or Bojun Ma.

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Xifeng Chen, Liang Hao and Jianwei Pan contributed equally to this work.

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Chen, X., Hao, L., Pan, J. et al. SPL5, a cell death and defense-related gene, encodes a putative splicing factor 3b subunit 3 (SF3b3) in rice. Mol Breeding 30, 939–949 (2012). https://doi.org/10.1007/s11032-011-9677-4

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